| Project/Area Number |
07670809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | TEIKYO UNIVERSITY |
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Principal Investigator |
TAKESHITA Satoshi TEIKYO UNIVERSITY,SCHOOL OF MEDICINE Assistant Professor, 医学部, 助手 (90271288)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | angiogenesis / collaterals / growth factor / grwoth factor / angiogenic grocoth factor / Collaterals / VEGF |
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| Research Abstract |
Proliferation of vascular cells constitutes an integral partof collateral artery development. We shought to determine the extent to which proliferative activity of vascular cells is augmented during therapeutic angiogenesis with vascular endothelial growth factor (VEGF). Endothelial cell (EC) proliferation in the midzone collaterals (especially those<100mum n diameter) of VEGF-treated animals increased 2.8-fold at day 5 (p<0.05, vs.control), and returned to baseline levels by day 7.
To confirm that such EC proliferation of small vessels with diameter <100mum contributes to the development of collaterals, we employed previously validated synchrotron radiation microangiography system with a spatial resolution of 30mum, and evaluated the development of small collaterals following therapeutic angiogenesis with VEGF.The number of angiographically visible collaterals with the diameter of less than 100mum was significantly higher in animals treated with VEGF compared with controls. This study clearly demonstrated the augmented EC proliferation and the development of small collateral artery network post-VEGF treatment.
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