The analyzes of the alteration and activity of protein kinase C in the epileptic mouse brain.
Grant-in-Aid for Scientific Research (C)
|Research Institution||OSAKA CITY UNIVERSITY|
古塚 大介 大阪市大, 医学部, 講師 (70199438)
KATSUMOTO Eiichi Osaka City University Medical School, Research Associate, 医学部, 助手 (90271189)
KIOKA Tetsuro Osaka City University Medical School, Research Associate, 医学部, 助手 (40254396)
横谷 昇 大阪市立大学, 医学部, 講師 (10244641)
|Project Fiscal Year
1995 – 1997
Completed(Fiscal Year 1997)
|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1997 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1996 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1995 : ¥900,000 (Direct Cost : ¥900,000)
|Keywords||protein kinase C / convulsion / translocation / epilepsy / Translocation / ep:lespy|
1.The alteration after PTZ-induced convulsion
The cytosolic PKC activity in the cerebral cortex significantly increased 10 min after seizure, compared with that of the control and tonic-convulsive mouse. On the other hand, that in the cortical membrane fraction after convulsion didn't alter significantly. PTZ-induced convulsion didn't considerably change the PKC activity of the cytosolic and membrane fractions in hippocapmus, striatum and thalamus/hypothalamus.
Western blot analyzes of PKC isozymes
The content of 80-K PKC beta in the cytosolic fraction increased, whereas that in the membrane fraction decreased at 10 min after convulsion. The 50-K PkC beta was detected in the cytosolic fraction during the tonic convulsion and 10 min after the convulsive phase. The expression of PKC alpha was similar to that of PKC beta. However the expressions of PKC gamma, delta, and zeta didn't alter after convulsions.
The cortical Calpain activities in the cytosolic and membrane fraction didn't change significantly after convulsions.
2.The alteration after EL mouse's seizure
(1)RT-PCR analysis of PKC isozymes expression
The mRNA expression of PKC alpha was slight at the interictal period and decreased gradually after the seizure. That of PKC beta was slight at the interictal period but increased gradually after the seizure. That of PKC gamma was abundant at the interictal period, reached a peak at 30 min after seizures and return the basal level at 120 min. That of PKC delta was little at the interictal period and peaked at 30 min after the seizure.
PKC alpha and beta translocated from membrane to cytosol in the cerebral cortex after PTZ-induced seizures. The alterations of PKC isozymes after seizures of EL mouse were different from those after PTZ-induced seizures. Further examinations are necessary by northern and western blot analyzes.
また、RT-PCRによるELマウス発作後のPKC isozumeの変化は、PTZ痙攣後の結果とは異なる発現パターンを示し、さらにnorthernおよびwestern blotによって検討したい。
Research Output (15results)