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GENE MAPPING OF MOYAMOYA DISEASE

Research Project

Project/Area Number 07671503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE

Principal Investigator

MATSUSHIMA Yoshiharu  TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助教授 (20134679)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kiyotaka  TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF CELL BIOLOGY,CHIEF RES, 細胞生物部門, 主任研究員 (90073022)
AOYAGI Masaru  TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,LECTURER, 医学部, 講師 (40134704)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsmoyamoya disease / familial occurence / gene analysis / muscle, smooth / arterial wall / clastin gene / human leukocyte antigen
Research Abstract

(1) We investigated the familial occurence of moyamoya disease using magnetic resonance angiography (MRA). We collected three familial moyamoya pedigrees. We collected blood samples from moyamoya patients and normal subjects in these families, and are now performing linkage analysis using the genomic DNA.The final results has not been obtained. However, much larger pedigrees may be necessary for the sucessful gene mapping of this disease. (2) We performed typing for human leukocyte antigen (HLA) in 32 unrelated moyamoya patients and compred with 178 sontrol subjects. We found a significant association of HLA B51 with moyamoya disease. The finding suggests that there may be a genetic predisposition for moyamoya disease and that host factors may play a role in the development of intimal thickening in early childhood. (3) WE histologically examined superficial temporal arteries (STA) of moyamoya patients by electron microscopy and immunohistochemistry. Intimal thickenings appeared significantly at an early age in moyamoya patients compared with control subjects. Intimal thickening in STA of moyamoya patients contained strongly stained elastic fibers. Tropoelastin gene transcripts was significantly greater in intimal smooth muscle cells (SMCs) of moyamoya patients than in control subjects. The cultured SMCs from moyamoya STA showed higher elastin synthesis and tropoelastin gene transcripts than those from control arteries. This may indicate alterlations in elastic gene regulations in moyamoya SMCs.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Aoyagi M,Ogami K,Matsushima Y,Shikata M,Yamamoto M,Yamamoto K.: "Human leukocyte antigen in patients with moyamoya disease." Stroke. 26. 1995 (415-417)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Fukai N,Ogami K,Yamamoto M,Yamamoto K.: "Kinetics of ^<125>I-PDGF binding and down-regulation of PDGF receptorin human arterial smooth muscle cell starins during cellular senescence in vitro." J Cell Physiol. 164. 1995 (376-384)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,Wakimoto H,Azuma H,Hirakawa K,Yamamoto K.: "Immunohistochemical detection of Ki-67 in replicative smooth muscle cells of rabbit carotid arteries after balloon denudation." Stroke. 26. 1995 (2328-2332)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamto M,Nakamura H,Yamamto M,Aoyagi M,Yamamoto K.: "Retardation of phenotypic transition of rabbit arterial smooth muscle cells in three-dimensional primary culture." Exp Cell Res. 225. 1996 (12-21)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Funaki N,Yamamoto M, Nakagawa K,Matsushima Y,Yamamoto K.20GB05:Early development of intimal thickening in superficial temporal arteries in patients with moyamoya disease.: Stroke. 27. 1996 (1750-1754)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,Azuma H,Niimi Y,Tajima S,Hirakawa K,Yamamoto K.: "Smooth muscle cell proliferation, elastin formation, and tropoelastin transcripts during the development of intimal thickening in rabbit carotid arteries after endothelial denudation." Histochemistry. 107. 1997 (11-18)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsushima Y WB Saunders: "Moyamoya dsiease." In : Neurological surgery. Youmans JR ed., 1202-1223 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Ogami K,Matsushima Y,Shikata M,Yamamoto M,Yamamoto K: "Human leukocyto antigen in patients with moyamoya disease." Stroke. 26. 415-417 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Fukai N,Ogami K,Yamamoto M,Yamamoto K.: "Kinetics of ^<125>I-PDGF binding and down-regulation of PDGF receptor in human arterial smooth muscle cell starins during cellular senescence in vitro" J Cell Physiol. 164. 376-384 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,Wakimoto H,Azuma H,Hirakawa K,Yamamoto K: "Immunohistochemical detection of Ki-67 in replicative smooth muscle cells of rabbit carotid arteries after balloon denudation." Stroke. 26. 2328-2332 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto M,Nakamura H,Yamamoto M,Aoyagi M,Yamamoto K: "Retardation of phenotypic transition of rabbit arterial smooth muscle cells in three-dimensional primary culture." Exp Cell Res. 225. 12-21 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Fuaki N,Yamamoto M,Nakagawa K,Matsushima Y,Yamamoto K: "Early development of intimal thickening in superficial temporal arteries in patients with moyamoya disease." Stroke. 27. 1750-1754 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Yamamoto M,Azuma H,Niimi Y,Tajima S,Hirakawa K,Yamamoto K: "Smooth muscle cell proliferation, elastin formation, and tropoelastin transcripts during the development of intimal thickening in rabbit carotid arteries after endothelial denuation." Histochemistry. 107. 11-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsushima Y,WB Saunders: Moyamoya disease. In : Neurological surgery. Youmans JR ed.1202-1223 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aoyagi M,Fuaki N,Yamamoto M,Nakagawa K,Matsushima Y,Yamamoto K.: "Early development of intimal thickening in superficial temporal arteries in patients with moyamoya disease." Stroke. 27. 1750-1754 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamto M,Nakamura H,Yamamto M,Aoyagi M,Yamamoto K.: "Retardation of phenotypic transition of rabbit arterial smooth muscle cells in three-dimensional primary culture." Exp Cell Res. 225. 12-21 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohno K,Matsushima Y,Toriyama H,Nariai T,Suzuki R,Hirakawa K.: "Surgical approach for treatment of ischemic cerebral stroke other than Moyamoya disease in children." Acta Neurochir (Wien). 138. 1218-1223 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aoyagi M,Yamamoto M,Azuma H,Niimi Y,Tajima S,Hirakawa K,Yamamto K.: "Smooth muscle cell proliferation,elastin formation,and tropoelastin transcripts during the development of intimal thickening in rabbit carotid arteries after endothelial denudation." Histochemistry. 107. 11-18 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aoyagi M,Yamamoto M,Azuma H,Nagashima G,Niimi Y,Hirakawa K,Yamamoto K.: "Expression of p53 protein and p53 gene transcripts in rabbit carotid arteries after balloon denudation." Histochemistry. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamoto M,Aoyagi M,Azumi H,Yamamoto K.: "Changes in osteopontin mRNA expression during phenotypic transition of rabbit arterial smooth muscle cells in primary culture." Histochemistry. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松島善治: "11.9章 モヤモヤ病 (坪川孝志、高倉公明、菊池晴彦 編)" 最新脳神経外科学、朝倉書店, 777-786 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aoyagi M.,Yamamoto M.,et al.: "Immunohistochemical detection of Ki-67 in replicative smooth muscle cells of rabbit carotid arteries after balloon denudation." Stroke. 26. 2328-2332 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoyagi M.,Fukai N.,et al.: "Kinetics of^<125> I-PDGF binding and down-regulation of PDGF receptor in human arterial smooth muscle cell starins during cellular senescence in vitro." J Cell Physiol. 164. 376-384 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aoyagi M.,Matsushima Y.,et al.: "Human leukocyte antigen in patients with moyamoya disease." Stroke. 26. 415-417 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto K.,Aoyagi M.,et al.: "Changes in elastin-binding proteins during the phenotypic transition of rabbit arterial smooth muscle cellsin primary culture." Exp Cell Res. 218. 339-345 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto M.,Yamamoto K.,et al.: "Identification of integrins in cell adhesion to native and denatured type I collagens and the phenotipic transition of rabbit arterial smooth muscle cells." Exp Cell Res. 219. 249-256 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamada I.,Matsushima Y.,et al.: "Moyamoya disease:comparison of assessment with MR angiography and MR imaging versus conventional angiography." Radiology. 196. 211-218 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsushima Y.: "Moyamoya dsiease.In:Neurological surgery.Youmans JR ed." WB Saunders., 1202-1223 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松島善治: "モヤモヤ病の手術" にゅ-ろん社, 48 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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