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¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1996 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1995 : ¥1,300,000 (Direct Cost : ¥1,300,000)
The response to intra-articularly injected plastic particles and their drainage on reactive granulation in the rat knee joints was examined by light and electron microscopy. These knee joints and associated iliac lymph nodes were excised after 5 minutes and also after intermediate periods until 3 months after injection of latex (1mum) or fluoresbrite particles (0.2mum or 10mum). Particles in the lymphatic or blood vessels were successfully substantiated by an enzymc-histochemical method (5'-nuclcotidase staining). Five minutes after the injection, most of the particles were scattered on the surface of the synovial membrane, and some particles were phagocytosed by synovial lining cells. After 5 hours, neutrophils phagocytosed particles which adhered to fibrin in the joint cavity. Twelve hours later, after the neutrophils had died, those same particles were phagocytosed by macrophages in the joint cavity. After 1 day, fluoresbrite particles (0.2mum) phagocytosed by macrophages were found
in the iliac lymph nodes. Whereas latex particles (1mum) were detected in the iliac lymph nodes after 3 days. Some fluoresbrite particles (10mum) were seen in the 5'-nucleotidase-positive lymphatic vessels in the synovial membrane. After 3 months, many macrophages full of particles formed granulation in the synovial membrane, and macrophages with phagocytosed particles were also seen increasingly in the iliac lymph nodes. Our findings suggested that ncutrophils and macrophages phagocytosed injected particles in the joint cavity, and that the macrophages brought the particles into the deep layr of the synovial membrane. Phagocytic macrophages also carried the particles to the iliac lymph nodes through lymphatic vessels in the synovial membrane. There were no morphological differences in the process of granulation and drainage between two sizes of plastic particles (1mum and 0.2mum) except the behavior of macrophages phagocytosing the particles.
平成7年度に報告した,ヒト(慢性関節リウマチ,変形性膝関節症)およびニホンザルの関節滑膜のリンパ管,血管の分布(International Orthopaedics,Vol.19,396-402)をふまえ,動物モデルとして正常膝関節(ラット)関節内にlatex,fluoresbriteをトレーサーとして注入し,経時的に走査型電子顕微鏡および透過型電子顕微鏡を駆使し観察した.その結果,関節内に注入された粒子は関節内で好中球およびマクロファージに貪食されること,マクロファージによって滑膜内に運ばれ肉芽組織の一部となること,さらに粒子の一部はマクロファージにより滑膜の5^l-nucleotidase陽性のリンパ管を経由し所属リンパ節へ運ばれることなどが明らかとなった.これらの結果はJournal of Orthopaedic Surgery誌(Vol.2,1997,in press)にて報告した.