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Basic research to establish a treatment for asthenozoospermic patients.

Research Project

Project/Area Number 07671725
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKobe Universuty School of Medicine

Principal Investigator

OKADA Hiroshi  Kobe University School of Medicine, Lecturer, 医学部附属病院, 講師 (00177057)

Co-Investigator(Kenkyū-buntansha) FUJISAWA Masato  Kobe University School of Medicine, research advisor, 医学部附属病院, 助手 (30243314)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsasthenozoospermia / male infertility / sperm motility / sperm function / respiratory oxygen species / pentoxifylline / SOD / asthenozoospermia, / male infertility, / sperm motility, / sperm funciton, / respiratory oxygen species, / pentoxifylline, / 活性酸素種 / 男性不妊 / 精子無力症
Research Abstract

Purpose : (1) To determine the incidence of reactive oxygen species (ROS) formation by spermatozoa from asthenozoospermic patients and the relationship between ROS formation and sperm motion parameters. (2) To assess the efficacy of in vitro and in vivo pentoxifylline treatment of asthenozoospermic patients whose spermatozoa generated high ROS levels.
Materials and Methods : (1) ROS formation by spermatozoa from asthenozoo-spermic patients and fertile volunteers was measured by chemiluminescence. ROS formation by the sperm preparations was investigated without stimulation (steady state) or after stimulation with N-formyl-methionyleucyl-phenylalanine (f-MLP) or phorbol-12-myristate-13-acetate (PMA). (2) Spermatozoa from 15 asthenozoospermic patients, whose spermatozoa produced high levels of ROS at steady state, were treated in vitro with pentoxifylline to determine its effect on ROS generation and sperm motion parameters. (3) These same 15 patients and 18 asthenozoospermic patients whos … More e spermatozoa did not produce ROS at steady state were treated with pentoxifylline at two different dosages (300 mg and 1200 mg/day) to determine its effect on ROS generation, sperm motion parameters and sperm fertilizing ability in vivo.
Results : (1) When the steady state ROS formation was detected without stimulation and that was not stimulated by f-MLP,the source of ROS could be attributed to the spermatozoa themselves. (2) Spermatozoa from 15 out of 71 asthenozoospermic patients generated ROS at steady state. These 15 patients were assigned to group A patients. (3) Pentoxifylline decreased ROS generation by spermatozoa from group A patients and preserved the decrease of curvilinear velocity and beat cross frequency for 6 hrs in vitro. (4) For group A patients orally administered pentoxifylline failed to decrease ROS generation by spermatozoa. For group A patients orally administered pentoxifylline had no effect on sperm motility, sperm motion parameters and sperm fertilizing ability at low dosage (300mg/day). It increased motility and beat cross frequency at high dosage (1200mg/day), but had no effect on sperm fertilizing ability in group A patients.
Conclusions : Stimulation of sperm preparations with f-MLP can identify the source of ROS generated at steady state. Among asthenozoospermic patients, there was a group of patients (group A) whose spermatozoa produces detectable steady state levels of ROS.For this group, pentoxifylline appeared to be effective for reducing ROS formation and preserving sperm motion parameters in vitro. Orally administered pentoxifylline had no effect at low dosage, however, it increased sperm motility and some sperm motion parameters without altering sperm fertilizing ability at high dosage.
Future : Currently, there is no effective treatment for patients with deficient sperm functions, such as idiopathic asthenozoospermia. To develop new treatment strategies, it is important to learn more about the nature of these deficiencies. The present results may provide clues to a therapeutic approach for the treatment of idiopathic male-factor infertility. For the confirmation of these results, a prospective and double-blind clinical trial, dealing with fecundity as the crucial outcome measure, is necessary. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Hiroshi Okada, Noboru Tatsumi, Masanori Kanzaki, Masato Fujisawa, Soichi Arakawa, Sadao Kamidono: "Formation of reactive oxygen species by spermatozoa from asthenozoospermic patients : response to treatment with pentoxifylline." Journal of Urology. vol.157(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Okada, Sadao Kamidono: "Reactive Oxygen species and its scavenger of semen from fertile and infertile men" In. Frontiers in Endocrinology. vol.14. 625-630 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Okada, Noboru Tatsumi, Masanori Kanzaki, Masato Fujisawa, Soichi Arakawa, Sadao Kamidono.: "Formation of reactive oxygen species by spermatozoa from asthenozoospermic patients : response to treatment with pentoxifylline" Journal of Urology. vol.157 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Okada, Sadao Kamidono: "Reactive Oxygen species and its scarvenger of semen from fertile and infertile men." In.Frontiers in Endocrinology vol.4-Perspectives on Assisted Reproduction-Ares-Serono Symposia Publiations.625-630 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Okada,Noboru Tosumi,Masanori Kanzaki,Masato Fujisawa,Soichi Arakawa,Sadao Kamidono: "Formation of reactive oxygen species by spermatozoa from asthenozoospermic patients : response to treatment with pentoxifylline" Journal of Vrology. vol.157 (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroshi Okada,Sadao Kamidono: "Reactive Oxygen species and its scarvenger of semen from fertile and infertile men." In Frontiers in Endocrinology. vol.4. 625-630 (1994)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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