| Project/Area Number |
07671926
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | NAGASAKI UNIVERSITY |
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Principal Investigator |
TSUDA Yasuo Nagasaki University, School of Medicine Hospital, Assistant, 医学部・附属病院, 助手 (30253660)
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| Co-Investigator(Kenkyū-buntansha) |
OHIRA Akihiro Nagasaki University, School of Medicine, Assistant Professor, 医学部, 助教授 (00169054)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Endothelins / ET-1 / ET-3 / ET_A receptor / ET_B receptor / immunohistochemistry / ETA受容体 / ETB受容体 / Muller細胞 / neurotransmitter |
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| Research Abstract |
We investigated the role of Endothelins in the neurotransmission of the retina by the immunohistochemistry. The eyeballs of Spraque-Dawley albino rats were enucleated. The tissues were stained with anti-ET-1 and anti-ET-3 antibody for immunohistochemical examination. ET-1 and ET-3 expression was observed in the neural retina and retinal pigment epithelial cells except the inner segment of photoreceptor cells. ET-3 immunoreactivity was observed between the outer and inner nuclear layr nuclei. This labeling was related to Muller cells. We suggest that ET-3 function as a general intercellular messenger, either directly as a neurotransmitter or indirectly as a modulator of neurotransmission by Muller cells. The tissues were also stained with anti-ET_A anti-ET_B antibody for immunohistochemical examination. ET_A receptor was observed in the whole retina, ET_B receptor was observed in the ne rve fiber layr and the inner plexiform layr. This suggest that ET_B receptor play the role of neurotransmission in the ganglion cells.
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