| Project/Area Number |
07672110
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
補綴理工系歯学
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
KUBOKI Takuo (1996) Okayama University・Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (00225195)
矢谷 博文 (1995) 岡山大学, 歯学部, 助教授 (80174530)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Atsushi Okayama University・Dental School Professor, 歯学部, 教授 (00066995)
松香 芳三 岡山大学, 歯学部, 助手 (90243477)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
|
|---|
| Keywords | Chondrocytes / Mechanical Stress / Cartilage Degradation Factors / Proteoglycan / Cyclic Stress / Matrix Metalloproteinase / 変形性顎関節症 / サイトカイン / メタロプロテアーゼ / メタロプロテアーゼインヒビター / 線維芽細胞 / 細胞培養 / 負荷 |
|---|
| Research Abstract |
Excessive mechanical stress has been proposed as a cause breakdown of the articular cartilage. To investigate the effect of mechanical stress on chondrocyte metabolism, we examined the extracellular matrix synthesis and the gene expression of cartilage degradation factors and growth factors.
1.15 kPa cyclic mechanical stress (CTF) caused less increase in proteoglycan (PG) synthesis than 5 kPa (230.3 and 179.1% increase at 5 and 15 kPa)
2.5 kPa of CTF caused more decrease in DNA synthesis (50.0 and 30.1% decrease, at 5 and 15 kPa).
3.The mean normalized IL-1beta mRNA level against the corresponding control transiently increased during both level of CTF.
4.The mean normalized bFGF mRNA level against the corresponding control transiently increased during 15 kPa of CTF,while remarkable change was not observed at 5 kPa of CTF.
5.The mean normalized MMP-9 mRNA level against the corresponding control progressively increased during 15 kPa of CTF,while the transient increase was observed during 5 kPa of CTF.
6.Mean normalized MMP-2, TIMP-1, agrecan, collagen type II and type X mRNA level against the control did not show remarkable change at the both level of CTF.
7.When 15 kPa of CTF was applied, the production level of pro-MMP-9 did not change, but the level of active form MMP-9 was slightly increased, while 5 kPa of CTF was applied, the production level of pro-MMP-9 did not show remarkable change and it was not activated to active form MMP-9. Both level of CTF did not change the production of MMP-2.
|
