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Expressions and Functions of Heat Shock Proteins in Orthodontically Moving Tooth

Research Project

Project/Area Number 07672208
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTohoku University

Principal Investigator

SHIMIZU Yoshiyuki  Tohoku University, Hospital of Dentistry, Tohoku University Assistant Profesor, 歯学部・附属病院, 講師 (20187470)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Yoshinobu  Tohoku University, School of Dentistry, Tohoku University Associate Profesor, 歯学部, 助教授 (20005078)
MITANI Hideo  Tohoku University, School of Dentistry, Tohoku University Profesor, 歯学部, 教授 (50014220)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsHeat shock protein / Periodontal ligament fibroblasts / Mechanical stress / HSP 27 / HSP 47 / HSP 60 / HSP 70 / HSP 90 / 矯正的歯の移動 / 骨芽細胞
Research Abstract

The purpose of this study was to examine the induction of various heat shock proteins by mechanical stress in human periodontal ligament fibroblasts (PDL). PDL were obtained from freshly extracted human premolars. Small peaces of periodontium were taken from middle one-third of the root and incubated in alpha-MEM medium containing 10% fatal bovine serum. Outgrowing fibroblast-like cells were subcultured and seeded into 6-well culture plates or 6-well flexible silicon plate coated with type I collagen. At the confluent phase, 1,2,4,6g/cm^2 of continuous compressive stress or 1 sec stretching/1 sec relaxation, maximum 15%elongation of cyclic stretching stress was applied for 72 hours. At the end of stimulation period, the cells were washed with PBS and fixed with TCA.The cells were sclaped and homogenized in Urea-Triton-X solution. The cell homogenate was subjected into 10 or 15% SDS polyaclylamide gel electrophoresis and immunoblotted against anti-HSP 27,47,60,70,90 antibody. Expressions of HSP 47,60,70 were slightly stimulated by continuous compressive stress after 20-30 minutes of stimulation and were decreased to equal or lower intense of 0 time control. In contrast, expression of HSP 27 was slightly inhibited but almost constant during 120 minutes stimulation. Expressions of HSP60 and 70 were almost constant after recovery for 72 hours. On the other hand, expression of HSP 47,60 and 70 were stimulated by cyclic stretching stress after 12 hours of stimulation. Expressions of these HSP were increased to the end of incubation for 96 hours. These results indicated expressions of HSP were controlled by mechanical stress in PDL and HSP may play an important role in the reconstruction process of a periodontium of orthodontically moving tooth.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] C.B.Wu and Yoshiyuki Shimizu: "Microsomal Casaein II in Endoplasmic Reticulum-and Golgi Apparatus-Rich Fractions of ROS 17/2.8 Osteoblast-Like Cells." Calcified Tissue Intemrnational. 57. 285-292 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshinobu Shimizu,et al: "Suppressing Activity of Periodontal Ligament Cells on Formation of Osteoclast-like Cells." Joumrnal of Bone and Mineral Metabolism. 14(2). 65-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 清水義之、三谷英夫: "骨芽細胞様細胞MC3T3E1の分化成熟過程とカゼインキナーゼ活性について" 歯科基礎医学会雑誌. 37. 202 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 清水義信、清水義之、三木美麗、他: "In vitro培養したヒト抹消血リンパ球/単球から形成された破骨細胞様細胞と骨吸収窩." 歯科基礎医学会雑誌. 39. 55-63 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 清水義之、清水義信、千葉美麗、三谷英夫: "圧縮力によるヒト歯根膜線維芽細胞のストレスタンパク質発現の解析" 歯科基礎医学会雑誌. 38. 115 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.B.Wu and Yoshiyuki Shimizu: "Microsomal Casaein II in Endoplasmic Reticulum- and Golgi Apparatus- Rich Fractions of ROS 17/2.8 Osteoblast-Like Cells." Calcified Tissue International. 57. 285-292 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshinobu Shimizu, et al.: "Suppressing Activity of Periodontal Ligament Cells on Formation of Osteoclast-like Cells." J Bone and Mine Metabolism. 14 (2). 65-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiyuki Shimizu and Mitani Hideo: "Role of casein kinase activities in the function of MC3T3E1 osteoblast-like cells." J pn J Oral Biol. 37. S202 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshinobu Shimizu, Yoshiyuki Shimizu, et al: "Formation of Osteoclast-like Cells and Pits in in Vitro Culture of Peropheral Blood Lymphocytes/Monocytes" J pn J Oral Biol. 39 (in press). 55-63 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshiyuki Shimizu, Yoshinobu Shimizu, et al.: "Expression of Heat Shock Proteins induced by compressive stress in human periodontal ligament fibrolasts" J pn J Oral Biol. 38. S115 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 清水 義信、清水 義之、三木 美麗、他: "In vitro培養したヒト抹梢血リンパ球/単球から形成された破骨細胞様細胞と骨吸収窩." 歯科基礎医学会雑誌. 39. 55-63 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 清水 義之、清水 義信、千葉 美麗、三谷 英夫: "圧縮力によるヒト歯根膜線維芽細胞のストレスタンパク質発現の解析" 歯科基礎医学会雑誌. 38. 115 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] C. B. Wu and Yoshiyuki Shimizu: "Microsomal Casaein II in Endoplasmic Reticulum- and Golgi Apparatus- Rich Fractions of ROS 17/2.8 Osteoblast-Like Cells." Calcified Tissue International. 57. 285-292 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 清水 義之、三谷 英夫: "骨芽細胞様細胞MC3T3E1の分化成熟過程とカゼインキナーゼ活性について" 歯科基礎医学会雑誌. 37. 202- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshinobu Shimizu: "Suppressing Activity of Periodontal Ligament Cells on Formation of Osteoclast-like Cells." Journal of Bone and Mineral Metabolism. 14(2). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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