Age Estimation Using the Racemization of Amino Acid on Human Teeth
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Single-year Grants |
|Research Institution||Kanagawa Dental College |
OHTANI Susumu Kanagawa Dental College Lecturer Department of Dentistry, 歯学部, 講師 (60104478)
|Project Period (FY)
1995 – 1997
Completed (Fiscal Year 1997)
|Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
|Keywords||Tooth / Dentin / Age Estimation / Amino acid / Racemization / D-aspartic acid / Gas chromatograph / 加齢変化 / 法医学 / 固定液 / エタノール / ホルマリン|
An investigation into racemization of aspartic acid in dentin (D/L ratio) was carried out for the realization of a more accurate dental age estimate.
1.Influence of pH :
The influence of pH (4 and 9) on teeth was investigated by a heating experiment for precise estimation of age at death. The reaction rate was highest in dentin in a pH 9 environment, followed by that in water, that in a pH 4 environment, and that in a dry state.
2.Influence of fixative :
We investigated and compared the degree of progression of racemization of dentinal aspartic acid in teeth stored in 95% ethanol, 10% formalin, or 10% neutral formalin fixatives. The racemization rate of dentinal aspartic acid in teeth stored in 10% neutral formalin was the highest, followed by that for teeth stored in 10% formalin then that for teeth stored in 95% ethanol. Teeth stored in these fixatives at 15ﾟC showed almost no progression of racemization.
3.Influence of pink teeth :
Age was estimated from pink teeth utilizing the racemization of dentinal aspartic acid. These results suggest that age estimated from pink teeth are probably underestimated but that a more accurate age estimate can be obtained after adequate washing.
4.Age estimation from racemization rate using heated teeth :
The ratio was higher in heated than in unheated teeth for total amino acid, but approximately the same for soluble peptide. These results suggest that age calculated from the D/L ratio of dentinal soluble peptide from burned bodies is sufficiently accurate for this parameter to be used to estimate age in teeth from burned bodies.
Report (4 results)
Research Products (39 results)