Interaction between heteromolecules-Structural study and application of PNA-
Grant-in-Aid for Scientific Research (C)
|Research Institution||Osaka University of Pharmaceutical Sciences (OUPS)|
DOI Mitsunobu OUPS,Pharmaceutics, Associate Prof., 薬学部, 助教授 (10183500)
ISHIDA Toshimasa OUPS,Pharmaceutics, Proffessor, 薬学部, 教授 (00111021)
TOMOO Koji OUPS,Pharmaceutics, Assistant, 薬学部, 助手 (70257898)
IN Yasuko OUPS,Pharmaceutics, Assistant, 薬学部, 助手 (50257896)
樽井 麻里子 大阪薬科大学, 薬学部, 助手 (60257897)
ASANO Akiko OUPS,Pharmaceutics, Subassistant, 薬学部, 副手 (40291801)
|Project Fiscal Year
1995 – 1997
Completed(Fiscal Year 1997)
|Budget Amount *help
¥2,300,000 (Direct Cost : ¥2,300,000)
Fiscal Year 1997 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1996 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1995 : ¥900,000 (Direct Cost : ¥900,000)
|Keywords||PNA / PEPTIDE / STRUCTURE / INTERACTION / HETEROMOLECULE / NUCLEIC ACID / HETEROMDLECULE|
Interactions between huge kinds of heteromolecules support the life of organs. The combination of simple interaction mode creates the complicated and functional structures. Therefore, the combination of interactions is very important for understanding function of bioactive compounds. Our investigation is focused on the interaction mode between nucleic bases and amino acids (protein).
One characteristics in this project is using peptidic nucleic acid (PNA) which is combined with amino acids sequences. This peptide is a hybrid dipeptide having peptide backbone and nucleic bases in the peptide. Our structural investigation gave the following results.
1 Basic unit of PNA
(1) N-(2-aminoethyl) glycine was synthesized from 3-amino-1,2-propanediol quickly.
(2) Substitution glycine for another amino acid make the distances between bases amino acid side chain shorter, and novel character was expected.
(3) Combination of alpha-PNA brings freedom in selecting the position of PNA in peptide.
2 Fluorescence titration of hybrid dipeptide
(1) The dipeptide containing cytosine base and tryptophan shows relatively high affinity for all nucleic bases.
3 X-ray crystal structure
(1) Dipeptide containing Trp crystallized as highly hydrate state
(2) Dipeptides containing Trp, Tyr, Ala, Phe and lle are associated in crystal
(3) Association level is very high in the dipeptides containing Phe and lle.
(4) Interaction between nucleic base and backbone (amino or carboxy group) are commonly observed in crystal structure.
Research Output (6results)