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Urine Protein profile System for Pathophysiologic Diagnosis of Renal and Urogenital Disorders

Research Project

Project/Area Number 07672496
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionJichi Medical School (JMS)

Principal Investigator

KAWAI Tadashi  Jichi Medical School, Dept.of Medicine, Professor, 医学部, 教授 (60048957)

Co-Investigator(Kenkyū-buntansha) ITOH Yoshihisa  Jichi Medical School, Dept.of Medicine, Associate Prof., 医学部, 助教授 (20129026)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsprotein 1 / alpha2-macroglobulin / hemoglobin / urine protein / basic fetoprotein / CR470 / urine protein profile / protein 1 / α2-マクログロブリン / RPPHS / ヘモグロビン / プロティン1 / 尿蛋白 / 塩基性蛋白 / CRM470
Research Abstract

The present project was successfully finished as planned before. During two years, the following research have been accomplished or are underway which are summarized as followed : a) Preparation of recombinant protein 1 and demonstration of its biological activity on inhibitory effects on phospholipase A2 and phospholipase C.Epitope analyzes are now under way. b) Immunohistochemical demonstration of protein 1 in epithelial cells of prostate and female prostate designated as Skene's gland. c) Demonstration of mechanisms of decreased serum protein 1 value in smokers due to decreased number of lung Clara cells and its decreased synthesis. d) Development of a latex agglutination photometric assay for urinary alpha2-macroglobulin. e) Evaluation of an Latex agglutination test for urinary basic fetoprotein (BFP) and discovery of the protein in semen structurally identical with native BFP.f) Establishment of BFP as a urinary marker for nonspecific inflammatory and tumor marker. g) Evaluation of a new international reference material for plasma proteins which gave the basis for future evaluation of a new reference material for urinary proteins. h) A study on urinary PSA-its evaluation of an assay and clinical application.
Based on these research works, urine protein profiles systems are being constructed with completion of reference intervals for each key proteins, and its expert system. Clinical trials by the system will be initiated within a year at latest.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] R Okutami: "Preparation and claracterization of human recombinant protein 1" Eur J clin chem clin Biochem.34. 691-696 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M Zaviaeic: "Immuno chemical localization ofhuman protein 1 in the female and male prostate" Histochem J. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N Shijubo: "Human clara cells are decreseed by smoking" Eur Respi J. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Itoh: "Serum protein standerdization in Japan" J Chin Lab Analjss. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山口哲司: "尿中α_2-マクログロブリンのラテックス免疫比濁測定法の確立" 臨床検査. 40. 855-858 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okutani R., Itoh Y., Yamada T., Yamaguchi T,Singh G., Yagisawa H., Kawai T.: "Preparatin and characterization of human recombinant protein 1/Clara cell Mr 10000 Protein." Eur J Clin Chem Clin Bochem. 34. 691-696 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Itoh Y., Ichihara K., Kanno T., Sugawara T., Ohkubo A., Hirabayashi Y., Igarashi S., Kawano K., Iwata S., Saito K., Kawai T.: "Serum Protein Standardization Project in Japan : Evaluation of an IFCC Reference Material (RPPHS/CRM470) and Establishment of Reference Intervals." J Clin Lab Analysis. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shijubo N., Itoh Y., Yamaguchi T., Shibuya Y., Fujishima T., Kawai T., Abe S.: "Human clara cells are decreased by smoking." Eur Respir J. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zaviacic M., Danihel L., Ruzicoa M., Biazekova J., Itoh Y., Okutani R., KawaiT.: "Immunohistochemical localization of fuman protein 1 in female prostate (Skene's gland) and the male prostate." Histochim J.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshihisa Itoh, Kazunori Sakabe, Tadashi Kawai: "Urinary basic fetoprotein in normal and pathologic urine." (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] R Okutani: "preparation and characyerization of human recombinant protein 1" Eur J Clin Chem Clin Biochem. 34. 691-696 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Zaviacic: "Immuno chemical localization of human protein 1 in the female and male prostate" Histochem J. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] N Shizuko: "Human clara cells are decreased by smoking" Eur Respi J. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y Itoh: "Serum protein standardization in Japan" J Clin Lab Analysis. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山口哲司: "尿中α2-マクログロブリンのラテックス免疫比濁測定法の確立" 臨床検査. 40. 855-858 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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