|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1996 : ¥1,000,000 (Direct Cost : ¥1,000,000)
Fiscal Year 1995 : ¥1,100,000 (Direct Cost : ¥1,100,000)
Chorioamnionitis (CAM) is considered as one of main causes of preterm delivery. It is reported that the fetal lung maturation of the premature infants delivered with CAM is accelerated compared with that of premature infants delivered without CAM.The parts of premature infants delivered with CAM are reported to diagnosed as having chronic lung disease including Wilson-Mikity syndrome. This chronic lung disease of premature infants exerts an influence on those prognoses. It is not clarified yet about the mechanism and substances that accelerate fetal lung maturation in CAM.Purposes of this study is to examine whether or not reactive oxygen species produced from the neutrophils that increase in amniotic fluid, involve as one of promotive substances of fetal lung maturation in CAM.
We infused xanthine 1mM+xanthine oxidase (100mU/ml, 10mU/ml, 1mU/ml, 0.1mU/ml) system (X/XO) and produced reactive oxygen species in amniotic fluid in 16th day of pregnant wister rats and gather fetal lungs in the 19th day of gestation. We measured surfactant associated protein A,B,C (sp-A,B,C) mRNA in fetal lung. Sp-A,sp-B,sp-C mRNA of the group that administered X/XO obviously increased in comparison with that of control group of the 19th day of gestation by RT-PCR method. Also, we observed effects of allopurinol and catalase which attenuated the expression of sp-A,B,C mRNA.In conclusions, reactive oxygen species in amniotic fluid by X/XO administration were considered as one of the important factors which accelerate fetal lung maturation.
(2)実験的検討では、Wister系妊娠ratを用い、妊娠16日目に開腹し、直視下にXanthine(X)1mM+Xanthine Oxidase(XO)100mu/ml,10mU/ml,1mU/ml,0.1mU/mlをそれぞれ羊水中に注入し、活性酸素を発生させた。妊娠19日目に再開腹し、胎児肺を摘出し組織中のsurfactant associated protein A,B,CのmRNAの発現について検討した。妊娠19日目におけるsurfactant associated protein A,B,CのmRNAをRT-PCR法で検討した結果、X/XOを投与した群でコントロール群に比較して明らかな増加が認められた。また、X/XOの拮抗物質であるcatalaseやallopurinolで一部抑制が観察された。