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Analysis on the developmental changes in molecular architecture of active zones

Research Project

Project/Area Number 07808086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIZOGUCHI Akira  KYOTO UNIVERSITY Dept.Anat.Graduate Sch, Assoc.Prof., 医学研究科, 助教授 (90181916)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsNerve regeneration / Growth cone / Synaptogenesis / Active Zone / Synaptic vesicle / Cell adhesion molecule / Membrane fusion / 成長円錐 / シナプス / 低分子量GTP結合蛋白質 / Rab3A / ニューレキシン
Research Abstract

The growth cone is a growing nerve tip that actively elongates toward the target cells at which it transforms into the synapse. The active extension of the growth cone is accompanied by expansion of the surface membrane that is probably mediated by the membrane fusion between the surface membrane and that of vesicles in the growth cone. By using F-actin overlay assay, we have recently found four proteins and named them as Neurabin I,Neurabin II,Afadin, and AF-6, which have one PDZ domain and are localized at synapses. Introduction of Neurabin I antisense RNA in neurons resulted in inhibition of neurite outgrowth. Afadin and AF-6 were localized at both adherens junctions and synapses. These results suggests that Neurabin I,Neurabin II,Afadin, and AF-6 may function as new adhesive systems at both adherens junctions and synapses. We have also found a new protein, Tomosin, which binds to syntaxin and may regulate the membrane fusion machinery in synapses as well as in the growth cones.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

Research Products

(24 results)

All Other

All Publications

  • [Publications] Fujita, Y., et. al: "Tomosin : A syntaxin-1-binding protein which dissociates Munc18/n-sec1 from syntaxin-1 and forms a novel complex in the neurotransmitter release process." Neuron. 20. 905-915 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoh, A., et. al: "Neurabin-II An actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adhesion sites." J. Biol. Chem.273. 3470-3475 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H., et. al: "Neurabin : A novel nerural tissue-specific actin filament-binding protein involved in neurite formation." J. Cell Biol.139. 951-961 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mandai, K., et. al: "Afadin : A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherense junction." J. Cell Biol.139. 517-528 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamatomto, T., et. al: "The Ras target AF-6 interacts with ZO-1 and serves as a pepripheral component of tight junctions in epithlial cells." J. Cell Biol.139. 785-795 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kinoshita, M., et. al: "Nedd-5 : A mammalian septin, is a novel cytoskeletal component interacting with actin-based structures." Genes and Development. 11. 1535-1547 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujita, Y., Shirataki, M., Sakisaka, T., Asakura, T., Ohya, T., Kotani, H., Yokoyama, S., Nishioka, H., Matsuura, Y., Mizoguchi, A., Sheller, H,R., and Takai, Y.Tomosin: "A syntaxin-l-binding protein which dissociates Munc18/n-secl from syntaxin-1 and forms a novel complex in the neurotransmitter release process." Neuron. 20. 905-915 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoh, A., Nakanishi, H., Obayashi, H., Wada, M., Takahashi, K., Satoh, K., Hirano, K., Nishioka, H., Hata, Y., Mizoguchi, A., and Takai, Y.: "Neurabin-II An actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adhesion sites." J.Bilo., Chem.273. 3470-3475 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi, H., Obashi, H., Satoh, Wada, M., Mandai, K., Satoh, K., Nishioka, H., Matsuura, Y., Mizoguchi, A., and Takai, Y.Neurabin: "A novel nerural tissue-specific actin filament-binding protein involved in neurite formation." J.Cell Biol.139. 951-961 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mandai, K., Nakanishi, H., Satoh, A., Obayashi, H., Wada, M., Nishioka, H., Itoh, M., Mizoguchi, A., Aoki, T., Fujimoto, T., Mausuda, Y., Tsukita, S., and Takai, Y.Afadin: "A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherense junction." J.Cell Biol.139. 517-528 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto, T., Harada, N., Kano, K., Taya, S., Canaani, E., Matsuura, Y., Mizoguchi, A., Ide, C., and Kaibuchi, K.: "The Ras target AF-6 interacts with ZO-1 and serves as a pepripheral component of tight junctions in epithlial cells." J.Cell Biol.139. 785-795 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kinoshita, M., Kumar, S., Mizoguchi, A., Ide, C., Kinoshita, A., Haraguchi, T., Hiraoka, Y., and Noda, M.: "Nedd-5 : A mammalian septin, is a novel cytoskeletal component interacting with actin-based structures." Genes and Development. 11. 1535-1547 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawano, S., Okajima, S., Mizoguchi, A., Tamai, K., Hirasawa, Y., and Ide, C.: "Immunocytochemical distribution of Ca2+-independent protein kinase C subtypes (delta, epsilon, zeta) in regenerating axonal growth cones of rat peripheral nerve." Neurosience. 81. 263-273 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matstani,M.,Mizoguchi,A.,Arii,T.,Iwasaki,T.,and ide.C.: "Localization of protein kinase C a.b and g-subspecies in sensory axon terminals of the rat muscle spindle." J.Neurocytol.23. 811-819 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okajima,S.,Mizoguchi,A.,Hirasawa,Y.,and lde.C.: "The distribution of protein kinase C(a.b.g subtypes)in the regenerating growth cone." Neuroscience.66. 645-654 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shibuya,Y.,Mizoguchi,A.,Takeichi,M.,Shimada,K.,and lde.C.: "Localization of N-cadherin in the normal and regenerating nerve fibers of the chicken peripheral nervous system." Neuroscience.67. 253-261 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawano S.et al.: "Immunocytochemical Distribution of Ca^<2+>-independent Protein kinase C Subtypes(δ,ε,andζ)in Regenerating Axonal Growth Gonos of Rat Perioleral Nerue" Neuroscience. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Akagi,S.et al.: "Localization of Synapsin I in normal and regenerating axonal sprouts of the rat sciatic nerve." Histochemistry.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Uehara K.,: "Localization of Synaptotagmin in the regenerating Sprouts emanatry from the nodes of Ranvier." Acta,Histochem.Cytochem.28. 401-407 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shibuya, Y., et al: "Localization of N-cadherin in the normal and regenerating nerve fibers of the chicken peripheral nervous system." Neuroscience. 67. 253-261 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Okajma, S., et al: "The distribution of protein kinase C(α,β,γ subtypes) in the regenerating growth cone." Neuroscience. 66. 645-654 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uehara, K., et al: "Localization of synaptotagmin in the regenerating sprouts emanating from the nodes of Ranvier." Acta Histochem. Cytochem.28. 401-407 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 溝口明: "免疫実験操作法(13章B,C,D)" 南江堂, 1236 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 溝口明: "GTP総合蛋白質" 羊土社, 312 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-03-31   Modified: 2016-04-21  

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