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AN ELASTIC PROTEIN IN CELL OF AORTA SMOOTH MUSCLE

Research Project

Project/Area Number 07833001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section時限
Research Field 血管生物学
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

KIMURA Sumiko  CHIBA UNIVERSITY,DEPARTMENT OF BIOLOGY,FACULTY OF SCIENCE,ASSOCIATE PROFESSOR, 理学部, 助教授 (50093232)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsSmooth Muscle of Aorta / Smooth Muscle of Gizzard / 900kDa Protein / High Molecular Weight Protein / 弾性タンパク質 / 血管平滑筋 / 900K Protein
Research Abstract

It is well known that a high melecular weight elastic protein, (connectin : 3,000 kDa) is present in striated muscle, but not in smooth muscle. However, a 900 kDa protein was detected by 2-6% SDS gel electrophoresis in smooth muscle.
The 900 kDa protein was extracted from aorta of bovine or chicken gizzard smooth muscle, with 0.1 M sodium phosphate buffer (pH 6.6). After the extract was dialyzed against 35 mM potassium phosphate buffer (pH 7.0), the protein was partially purified with DEAE column chromatography by gradient elution from 35 to 60 mM phosphate buffer. The purified protein from chicken gizzard crossreacted with the plolyclonal antibodies to the 900 kDa protein. The antibodies reacted with the 900 kDa band in the total SDS extract of both chicken aorta and gizzard smooth muscle.
The purified protein was observed under an electron microscope after rotary shadowing. The molecular shape of 900 kDa protein was long and very flexible, similar to vertebrate striated elastic protein (connectn).
Now cDNA sequence work is under way.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Sorimachi,H.,Kinbara,K.,Kimura,S.,et.al.: "Muscle-specific calpain,p94,responsible for limb girdle muscular dystrophy type 2A,associates with connectin through IS2,a p94-specific sequence." J.Biol.Chem.270. 31158-31162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kake,T.,Kimura,S.,Takahashi & Maruyama: "Calponin induces actin polymerization at low ionic strength and inhibits depolymerization of actin filaments." Biochem.J.312. 587-592 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maki,S.,Ohtani,Y.,Kimura,S.& Maruyama: "Isolation and characterization of a Kettin-like protein from crayfish claw muscle." J.Muscle Res.& Cell Motil.16. 579-585 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtani,Y.,Maki,S.,Kimura,S.,& Maruyama: "Localization of connectin-like proteins in leg and flight muscles of insects." Tissue & Cell. 28. 1-8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yajima,H.,Ohtsuka,H.et.al.: "A 11.5-kb 5'-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region." Biochem.Biophys.Res.Commun.223. 160-164 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtsuka,H.,Yajima,H.,Maruyama & Kimura,S.: "Binding of the N-terminal 63 kDa protein of connectin/titin to α-actinin as revealed by the yeast two-hybrid system." FEBS Lett.401. 65-67 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sorimachi, H., Kinbara, K., Kimura, S., Takahashi, M., Ishiura, S., Sasagawa, N., Sorimachi, N., Shimada, H., Tagawa, K., Maruyama, K.and Suzuki, K.: "Muscle-specific calpain, p94, responsible for limb girdle muscular dystrophy type 2A,associates with connectin through IS2, a p94-specific sequence." J.Biol.Chem.270. 31158-31162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kake, T., Kimura, S., Takahashi, K.and Maruyama, K.: "Calponin induces actin polymerization at low ionic strength and inhibits depolymerization of actin filaments." Biochem.J.312. 587-592 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maki, S., Ohtani, Y., Kimura, S.and Maruyama, K.: "Isolation and Characterization of A Kettin-like Protein from Crayfish Claw Muscle." J.Muscle Res.Cell Motil.16. 579-585 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawamura, Y., Kume, H., Itoh, Y., Ohtsuka, S., Kimura, S.and Maruyama, K.: "Localization of three fragments of connectin in chicken brease muscle sarcomeres." J.Biochem. 117. 201-207 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kake, T., Spissinger, T., Sato, O., Kimura, S.and Maruyama, K.: "Molecular shape of dystrophin with special references ot tupe VI collagen." Proc.Japan Acad.71, B. 24-26 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yajima, H., Ohtsuka, H., Kume, H., Endo, T., Maruyama, K., Kimura, S.and Maruyama, K.: "Molecular cloning of a partial cDNA clone encoding the C terminal region of chicken breast muscle connectin." Zool.Sci.13. 119-123 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtani, Y., Maki, S., Kimura, S., and Maruyama, K.: "Localization of connectin-like proteins in leg and flight muscles of insects." Tissue & Cell. 28. 1-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yajima, H., Ohtsuka, H., Kawamura, Y., Kume, H., Murayama, T., Abe, H., Kimura, S., and Maruyama, K.: "A 11.5-kb 5'-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region." Biochem.Biophys.Res.Commun.223. 160-164 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtsuka, H., Yajima, H., Maruyama, K., and Kimura, S.: "Binding of the N-terminal 63 kDa protein of connectin/titin to a-actinin as revealed by the yeast two-hybrid system." FEBS Lett T.401. 65-67 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohtsuka, H., Yajima, H., Maruyama, K.and Kimura, S.: "The N-terminal Z repeat 5 of connectin/titin binds to the C-terminal region of alpha-actinin." Biochem.Biophs.Res.Commun.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sorimachi,H.,Kinbara,K.,Kimura,S.,et.al.: "Muscle-specific calpain,p94,responsible for limb girdle muscular dystrophy type 2A,associates with connectin through IS2,a P94-specific sequence." J.Biol.Chem.270. 31158-31162 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kake,T.,Kimura,S.,Takahashi & Maruyama: "Calponin induces actin polymerization at low ionic strength and inhibits depolymerization of actin filaments." Biochem.J.312. 587-592 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maki,S.,Ohtani,Y.,Kimura,S & Maruyama: "Isolation and characterization of a Kettin-like protein from crayfish claw muscle." J.Muscle Res. & Cell Motil.16. 579-585 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohtani,Y.,Maki,S.,Kimura,S.,& Maruyama: "Localization of connectin-like proteins in leg and flight muscles of insects." Tissue & Cell. 28. 1-8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yajima,H.,Ohtsuka,H.et.al.: "A11.5-kb 5'-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region." Biochem.Biophys.Res.Commun.223. 160-164 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohtsuka,H.,Yajima,H.,Maruyama & Kimura,S.: "Binding of the N-terminal 63 kDa protein of connectin/titin to α-actinin as revealed by the teast ywo-hybrid system." FEBS Lett.401. 65-67 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sorimachi,Y.: "Muscle-specific calpain, p94, responsible for limb girdle muscular dystrophy type 2A, associates with connectin through IS2, a p94-specific sequence.21GC01:Journal of Biological Chemistry22GD01:270" 31158-31162 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kake,T.: "Calponin induces actin polymerization at low ionic strength and inhibits depolymerization of actin filaments." Biochemical Journal. 312. 587-592 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yajima,H.: "Molecular cloning of a partial cDNA clone encoding the C terminal region of chicken breast muscle connectin.21GC03:Zoological Sciences" 13. 119-123 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-25  

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