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木質炭化素材を用いたPOPs分解複合微生物系の構築とバイオレメディエーション

Research Project

Project/Area Number 07J07767
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Boundary agriculture
Research InstitutionFukuoka University (2008)
National Institute for Agro-Environmental Sciences (2007)

Principal Investigator

亀井 一郎  Fukuoka University, 工学部, 助教

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2008: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsバイオレメディエーション / 白色腐朽菌 / POPs / 木質炭化素材 / リグニン / ディルドリン / エンドスルファン / 微生物 / 微生物分解 / 環境汚染物質 / ダイオキシン / DDT / ヘキサクロロベンゼン
Research Abstract

本研究の最終目標は有害汚染物質の変換能に優れる担子菌と資化能に優れる細菌との複合微生物系を構築し、環境浄化資材としての可能性を検証することにある。対象化合物として近年問題となっている残留性有機汚染物質(POPs)に指定されているディルドリン、および今後POPsに指定される可能性があるエンドサルファンを用いた。まず、担子菌分離株ストックを用いて、ディルドリンを分解基質とした選抜を行った結果、未同定株YK543がディルドリンを分解可能であることが示された。また、GC/MS分析により代謝物が検出された。エンドスルファンを分解基質として選抜を行った結果、エンドスルファン、およびその主要な代謝物であるエンドスルファンサルフェートを分解可能な菌としてTrametes hirsutaが選抜され、複数の代謝物が検出された。エンドスルファンサルフェートはPhanerochaete chrysosporiumでは分解できないことが報告されている。代謝物について検討したところ、未同定の化合物を含む複数の代謝物が検出され、新規な分解経路の存在が示唆された。すなわち、安定で分解が困難なPOPsの構造を変換することに成功し、将来的に細菌との組み合わせによる完全分解系を構築するための布石となる成果を得た。

Report

(2 results)
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2009 2008 2007

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (16 results)

  • [Journal Article] Influence of soil properties on the biodegradation of 1, 3, 6, 8-tetrachlorodibenzo-p-dioxin and fungal treatment of contaminated paddy-soil by white rot fungus Phlebia brevispora2009

    • Author(s)
      Ichiro Kamei
    • Journal Title

      Chemosphere (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Saline-dependent regulation of manganese peroxidase genes in the hype rsaline-tolerant white rot fungus Phlebia sp. MG-602008

    • Author(s)
      Ichiro Kamei
    • Journal Title

      Applied and Environmental Microbiology 74

      Pages: 2709-2716

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of 1, 1, 1-trichloro-2, 2-bis (4-chlorophenyl) ethane (DDT) by brown rot fungi2008

    • Author(s)
      Adi Setyo Purnomo
    • Journal Title

      Journal of Bioscience and Bioengineering 105

      Pages: 614-621

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2008

    • Author(s)
      亀井一郎
    • Journal Title

      微生物の事典(朝倉書店)

      Pages: 593-594

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Saline-dependent regulation of manganese peroxidase genes in the hypersaline-tolerant white rot fungus Phlebia sp. MG-602008

    • Author(s)
      Ichiro Kamei
    • Journal Title

      Applied and Environmental Microbiology 74

      Pages: 2709-2716

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane(DDT)by brown rot fungi2008

    • Author(s)
      Adi Setyo Purnomo
    • Journal Title

      Journal of Bioscience and Bioengineering (掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The fungal treatment of the dioxin contaminated soil2007

    • Author(s)
      Ichiro Kamei
    • Journal Title

      Organohalogen Compounds 69

      Pages: 2584-2587

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic pathways of hexachlorobenzene(HCB), quintozene(PCNB), and pentachlorophenol by a newly isolated strain Nocardioides sp. PD653 under aerobic conditions2007

    • Author(s)
      Kazuhiro Takagi
    • Journal Title

      Organohalogen Compounds 69

      Pages: 2576-2579

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selection of Pleurotus pulmonarius from domestic basidiomycetous fungi for biodegradation of chlorinated dioxins as environmentally persistent organopollutants2007

    • Author(s)
      Muneyoshi Yamaguchi
    • Journal Title

      Bulletin of FFPRI 6

      Pages: 231-237

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 担子菌による環状ジエン系有機塩素農薬の分解2009

    • Author(s)
      亀井一郎
    • Organizer
      第59回日本木材学会大会
    • Place of Presentation
      松本
    • Related Report
      2008 Annual Research Report
  • [Presentation] 冬虫夏草(Cordyceps属)菌による多環式芳香族炭化水素および塩素化ダイオキシン分解の試み2008

    • Author(s)
      久野祐功
    • Organizer
      第53回リグニン討論会
    • Place of Presentation
      東京
    • Year and Date
      2008-10-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cordyceps 属(冬虫夏草菌)による有機塩素化合物汚染土壌浄化の試み2008

    • Author(s)
      久野祐功
    • Organizer
      第15回日本木材学会九州支部大会
    • Place of Presentation
      日田
    • Related Report
      2008 Annual Research Report
  • [Presentation] Metabolism of heavily chlorinated aromatic compQunds by Nocardioides sp. PD6532008

    • Author(s)
      Ichiro Kmaei
    • Organizer
      3^<th> International Conference on Soil Pollution and Remediation
    • Place of Presentation
      Nanjing, China
    • Related Report
      2008 Annual Research Report
  • [Presentation] Phlebia brevisporaによるダイオキシン汚染土壌浄化機構の解明2008

    • Author(s)
      江副裕介
    • Organizer
      日本木材学会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2007 Annual Research Report
  • [Presentation] Involvement of DDT-dechlorinase in Degradation of 1,1,1-Trichloro-2,2-bis-(4-chlorophenyl)Ethane(DDT)by Fomitopsis pinicola2008

    • Author(s)
      Adi Setyo Purnomo
    • Organizer
      日本木材学会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2007 Annual Research Report
  • [Presentation] Phlebia brevisporaによるダイオキシン汚染土壌浄化の試み2007

    • Author(s)
      江副裕介
    • Organizer
      日本木材学会九州支部大会
    • Place of Presentation
      久留米リサーチパーク(久留米市)
    • Related Report
      2007 Annual Research Report
  • [Presentation] 耐塩性白色腐朽菌Phlebia sp. MG-60におけるマンガンペルオキシダーゼ転写制御2007

    • Author(s)
      亀井一郎
    • Organizer
      リグニン討論会
    • Place of Presentation
      宇都宮大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] Degradation of 1,1,1-Trichloro-2,2-bis-(4-chlorophenyl)Ethane(DDT)by Brown Rot Fungus Fomitopsis pinicola2007

    • Author(s)
      Adi Setyo Purnomo
    • Organizer
      リグニン討論会
    • Place of Presentation
      宇都宮大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression analysis of manganese peroxidases from saline-tolerant white rot fungus, Phlebia sp. MG602007

    • Author(s)
      Ichiro Kamei
    • Organizer
      10th International Congress on Biotechnology in the Pulp and Paper Industry
    • Place of Presentation
      Monona Terrace(Madison, Wisconsin, USA)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Biodegradation pathways of dioxin and PCB, and treatment of dioxin-contaminated soil by white-rot fungus Phlebia brevispora2007

    • Author(s)
      Ichiro Kamei
    • Organizer
      10th International Congress on Biotechnology in the Pulp and Paper Industry
    • Place of Presentation
      Monona Terrace(Madison, Wisconsin, USA)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Possibility of Involvement of Fenton and Enzymatic reaction in Degaradation of 1,1,1-Trichloro-2,2-bis-(4-chlorophenyl)Ethane(DDT)by Brown Rot Fungi2007

    • Author(s)
      Adi Setyo Purnomo
    • Organizer
      10th International Congress on Biotechnology in the Pulp and Paper Industry
    • Place of Presentation
      Monona Terrace(Madison, Wisconsin, USA)
    • Related Report
      2007 Annual Research Report
  • [Presentation] The fungal treatment of the dioxin contaminated soil2007

    • Author(s)
      Ichiro Kamei
    • Organizer
      27th International Symposium on Halogenated Persistent Organic Pollutants-DIOXIN 2007
    • Place of Presentation
      Hotel OKURA(Tokyo)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Metabolic pathways of hexachlorobenzene(HCB), quintozene(PCNB), and pentachlorophenol by a newly isolated strain Nocardioides sp. PD653 under aerobic conditions2007

    • Author(s)
      Kazuhiro Takagi
    • Organizer
      27th International Symposium on Halogenated Persistent Organic Pollutants-DIOXIN 2007
    • Place of Presentation
      Hotel OKURA(Tokyo)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Degradation of Dioxin and PCB by White-Rot Fungus Phlebia brevispora2007

    • Author(s)
      Ichiro Kamei
    • Organizer
      The 4th International Medicinal Mushroom Conference-IMMC4
    • Place of Presentation
      The Cankarjev dom Cultural and Congress Centre(Ljubljana, Slovenia, EU)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Bioremediation potential of Nocardioides sp. PD6532007

    • Author(s)
      Kazuhiro Takagi
    • Organizer
      4th International Symposium on Persistent Toxic Substances
    • Place of Presentation
      Research Center for Eco-Environmental Sciences (Beijing, China)
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2024-03-26  

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