Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||Kyoto Institute of Technology|
TANAKA Nobuo Kyoto Institute of Technology, Faculty of Textile Science, 繊維学部, 教授 (60127165)
LIU Z Centers for Disease Control, Toxicology Branch, 公衆保健局・防疫センター, 研究員
BARR J.B Centers for Disease Control, Toxicology Branch, 公衆保健局・防疫センター, 研究員
SIRIMANNE S.R Centers for Disease Control, Toxicology Branch, 公衆保健局・防疫センター, 研究員
MOSBACH K Lund University, Chemical Center, 化学センター, 教授
BUZSEWSKI B.B Copernicus University, Department of Environmental Chemistry, 環境化学科, 教授
PATTERSON D.G Centers for Disease Control, Toxicology Branch, 公衆保健局・防疫センター, 室長
HOSOYA Ken Kyoto Institute of Technology, Faculty of Textile Science, 繊維学部, 助教授 (00209248)
OIDA Tatsuo Kyoto Institute of Technology, Faculty of Industrial Design and Engineering, 工芸学部, 助教授 (90152032)
OTSUKA Koji Himeji Institute of Technology, Faculty of Science, 理学部, 助教授 (70183762)
TERABE Shigeru Himeji Institute of Technology, Faculty of Science, 理学部, 教授 (50115888)
BARR J.B. 米国, 公衆保健局・防疫センター, 研究員
MOSBACH K ルンド大学, 化学センター, 教授
|Project Period (FY)
Completed(Fiscal Year 1996)
|Budget Amount *help
¥2,800,000 (Direct Cost : ¥2,800,000)
Fiscal Year 1996 : ¥2,800,000 (Direct Cost : ¥2,800,000)
|Keywords||Dioxin / PCB / Photolysis / HPLC stationary phase / EKC / Serum sample treatment / Aromatic hydrocarbons / Coplanar PCB / Pseudo-stationary phase|
The objectives of this project include (1) the development of a photolysis method of dioxins and PCBs in aqueous waste, (2) the development of a high selectivity separation method of dioxin isomers and PCB congeners by HPLC,and (3) the development of a novel pseudo-stationary phase for the high-speed and high-selectivity EKC separation of environmental contaminants, namely hydrophobic hydrocarbons and halogenated aromatic compounds.
(1) PCBs were successfully adsorbed from aqueous solution onto the hydrophobic stationary phase packed in a quartz column, the photolyzed by the irradiation with ultra-violet light from outside the column. All the PCBs can be completely photolyzed in a few hours in a stream of 2-propanol-water mixtures, while only hydrophilic products leaving the column. The photolysis of more toxic, coplanar PCBs were slower than ortho-substituted PCBs. The rapid photo-decomposition of less toxic, ortho-substituted PCBs, however, preferentially produced more toxic coplanar
PCBs as an intermediate, which can potentially provide serious environmental problems, if similar photolysis occurs under sunlight.
(2) The separation of all the dioxin isomers coproduced during the synthesis of reference standard and the group separation between coplanar and ortho-substituted PCBs were achieved by using various electron-donor and electron-acceptor bonded stationary phases based on silica gel for HPLC in an unmixed organic solvent. This allows the easy recycle of the organic solvent in preparative operation.
(3) Pseudo-stationary phases were prepared by using starburst dendrimers and polyallylamine as supports by alkylation and the attachment of carboxylate groups. The pseudo-stationary phases for EKC,especially those with long alkyl chains, showed very high performance in water-methanol or water-acetonitrile mixtures to result in the complete separation of 16 priority pollutant polynuclear aromatic hydrocarbons in less than 20 minutes.
The results suggest that the photolysis of PCBs already released to the environment is potentially very dangerous to living species, assuming a similar photo-decomposition under sunlight, and that the control and proper disposal of remaining PCBs are very important. The results also suggest that it is important to develop a more selective, sensitive, and rapid analysis method of these environmental pollutants that needs minimum labor for the treatment of biological samples. Less