| Co-Investigator(Kenkyū-buntansha) |
ITOH Yasuo Sinshu University, Faculty of Natural Science, Res.Associate, 理学部, 助手 (70283231)
AKIMITSU Kazuya Kagawa University, Faculty of Agriculture, Assoc.Professor, 農学部, 助教授 (80263888)
TSUGE Takashi Nagoya University, Graduate School of Life Science and Agriculture, Assoc, Profe, 大学院・生命農学研究科, 助教授 (30192644)
KODAMA Motoichiro Tottori University, Faculty of Agriculture, Assist.Professor, 農学部, 講師 (00183343)
OHTANI Hiroshi Tottori University, United Graduate School of Agricultural Sciences, Professor, 大学院・連合農学研究科, 教授 (50032305)
DUNKLE L.D. パデュー大学, 植物病理学科, 教授
GILCHRIST D. カルフォルニア大学, デービス校・植物病理学科, 教授
SIEDOW J.N. デューク大学, 植物学科, 教授
WOLPERT T.J. オレゴン州大学, 植物病理学科, 助教授
JOHAL G. ミズリー大学, 農学科, 助教授
TURGEON B.G. コーネル大学, 植物病理学科, 準教授
MACKO V. コーネル大学, ボイストムリン研究所, 教授
田平 弘基 鳥取県園芸試験場, 研究員
YODER O.C. コーネル大学, 植物病理学科, 教授
BRIGGS S.P. パイオニアHiーBred社, 生物工学研究所, 総括部長
WALTON J.D. ミシガン州立大学, 植物研究所, 準教授
宮川 恒 京都大学, 農学部, 助教授 (10219735)
朴 杓允 帝京大学, 医学部, 助手 (20147094)
荒瀬 栄 島根大学, 生物資源科学部, 助教授 (40127478)
BRONSON C.R. アイオア州立大学, 植物病理学科, 準教授
小林 裕和 静岡県立大学, 大学院・生活健康科学研究科, 助教授 (80170348)
中島 広光 鳥取大学, 農学部, 助教授 (40144646)
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| Budget Amount *help |
¥25,300,000 (Direct Cost: ¥25,300,000)
Fiscal Year 1998: ¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 1996: ¥7,800,000 (Direct Cost: ¥7,800,000)
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| Research Abstract |
Many fungal genes responsible for the producion of host-specific toxins were cloned from Alternaria and Cochliobolus pathogens. Some of them were elucidated thier structures and function in the course of US/Japan Joint Research Project. A cyclic peptide synthetase gene (4MT) for AM-toxin of leaf blotch of apple pathogen was found to bel3kb in length with no introns and have four domains of amino acid activation specific for the amino acids constituting the peptide toxin. The gene cluster involved in AK-toxin biosynthesis in the black spot pathogen of Japanese pear were made structural and functional analyses AKT1 (fatty acid synthesis), AKT3(modification of fatty acid), AKTR(regulator), AKTS1 (similar to threonine dehydratase, its true role is unclear, but specific to the Japanese pear pathotype) and AKT2 (no similarity to known proteins in the databases). The strawberry pathotye had AKTl AKT3 and AKTR homologues, and the tangerine pathotype possessed AKTR homologue. The shared distribution of the genes were compatible with the molecular similarity of toxins. Also, structure and function of TOX1 and TOX2 in Cochijobolus were elucidated in depth. A gene for the sensitivity to ACR-toxin was cloned from mtDNA of host rough lemon, and a protein specific to the AM-toxin sensitivity was isolated from susceptible apple leaves by proteome analysis. Most fungal genes for host specific toxin biosynthesis would be horizontally transferred.
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