Biological significance of Prolinerich antimicrobial peptide
Grant-in-Aid for International Scientific Research.
|Section||Joint Research .|
|Research Institution||Asahikawa Medical College|
KOHGO Yutaka Asahikawa Medical College, Medicine Professor, 医学部, 教授 (10133183)
GALLO Richar Joint Program of Neonatology, ハーバード大学, assistant
ASHIDA Tomofumi Asahikawa Medical College, Medicine, Instructor, 医学部, 助手 (50261409)
YOKOTA Kinichi Asahikawa Medical College, Medicine, Lecturer, 医学部, 講師 (10250573)
ONO Minoru Asahikawa Medical College, Medicine, Lecturer, 医学部, 講師 (60185650)
FUJIMOTO Yoshinori Asahikawa Medical College, Medicine, Instructor, 医学部, 助手 (90292127)
GALLO Richa ハーバード大学, 医学部, Assistant
RICHARD Gall ハーバード大学, 医学部, Assistant
GALLO Richard Harvard University, Medicine, Assistant Professor
|Project Fiscal Year
1996 – 1997
Completed(Fiscal Year 1997)
|Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 1997 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1996 : ¥1,600,000 (Direct Cost : ¥1,600,000)
|Keywords||antimicrobial peptide / PR-39 / syndecan / metastasis / cytoskeletone / ras sinal / 抗菌ペプチド / シンデカン / 癌転移 / 細胞骨格 / rasシグナル / 内因性抗菌ペプチド|
We clarified several important points about syndecans and PR-39 through colaborations and discussions with Dr.Gallo as shown below.
1.We made polyclonal antibody against PR-39 afer immunization of rabbits using N-terminal 15 aminoacids oigopeptides.
2.We observed the band at the size of approximately 5 kDa as expected size of PR-39 mature protein afer immunoprecipitation of human leukocytes protein using PR-39 polyclonal antibody. However, we have not cloned the protein which might be human counterpart of pig PR-39.
3.PR-39 has a possible function which can bind to Src homology 3 domain, becaus PR-39 has five repeats of proline rich motif that has been reported to bind SH3 domain as like a Sos protein which can bind to Grb2 protein. Recombinant SH3 domain of Src gene can actually bind to PR-39 oligopeptides by the study of immunopregipitation.
4.PR-39 gene transfection into human hepatoma cells showed induction of syndecan-1 expression, suppression of invasive activity and cell morphological change. These results might be due to the binding ability of PR-39 into SH3 domain.
5.PR-39 transfectans excreted PR-39 protein into conditioning medium.
6.PR-39 gene transfection into ras transformants also showed morphological change and decrease of proliferation rate.
Research Output (9results)