TROND Berg University of Oslo, Institute of Biology, 医学部, 教授
RUNE Blomhoff University of Oslo, Institute for Nutrition Research, 医学部, 教授
KAARE R Norum University of Oslo, Institute for Nutrition Research, 医学部, 教授
IMAI Katsuyuki Akita University, School of Medicine, Research Associate, 医学部, 助手 (80006741)
SATO Mitsuru Akita University, School of Medicine, Research Associate, 医学部, 助手 (60226008)
BERG Trond オスロ大学, 理学部, 教授
BLOMHOFF Run オスロ大学, 医学部, 教授
NORUM Kaare オスロ大学, 医学部, 教授
|Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥2,100,000 (Direct Cost: ¥2,100,000)
Seven Svalbard reindeers, 3 ringed seals, 2 bearded seals, 4 glaucous gulls, 3 Brunnich's guillemots, and 1 puffin were caught in the Svalbard archipelago from August through September, 1996. Fresh each organs, namely, the liver, kidneys, lungs, jejunum, spleen, and skin were investigated with gold chloride staining, fluorescence microscopy for autofluorescence of vitamin A,Sudan 3 lipid staining, hematoxylin and eosin staining, Azan staining, Ishii and Ishii's silver impregnation method, and transmission electron microscopy.
The Arctic animals and birds store a large amount of vitamin A in hepatic stellate cells. Only a small amount of vitamin A existed in other organs : kidneys, jejunum, spleen, lungs, and skin. On the other hand, extrahepatic stellate cells in normal rats were demonstrated to store vitamin A in the kidneys, lungs, spleen, and intestine.
These results indicated that the Arctic animals and birds can store a huge amount of vitamin A in hepatic stellate cells in physiological conditions. These data also indicated that the molecular mechanisms such as retinoid-binding proteins in the Arctic animals and birds play pivotal roles in their vitamin A storage. Applying these molecular mechanisms the prevention methods will be developed for hypervitaminosis A as a side effect of vitamin A treatment to malignant diseases such as leukemia.