CULLEN B.R. Duke University, Howard Hughes Medical In, Professor
HAMAJIMA Kenji YOKOHAMA CITY UNIVERSITY,SCHOOL OF MEDICINE,RESEARCH ASSISTANT, 医学部, 助手 (00114611)
CULLEN Bryanr DUKE UNIVERSITY MEDICAL CENTER,HOWARD HUGHES MEDICAL INSTITUTE,PROFESSOR
|Budget Amount *help
¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1996 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Our final aim of this research project is to develop the new type of immunization methods by naked DNA injection technique using HIV-1 antigen expressing plasmids against HIV-1 infection. We have firstly analyzed the HIV-1 transactivation effect of Bel-1 protein from human foamy virus. In this study, we found the DNA binding activity of Bel-1 protein, and the possibility of activation of HIV-1 replication in in vivo environment. Next we have analyzed the immunological effect of DNA immunization by naked plasmid injection (Aids Res.Hum.Retroviruses., 1995 11 : 933-943). The summary of results are as follows
1) We can detect the cytotoxic T cell (CTL) activity against Nef peptide using Nef expressing plasmid (Am.J.Hematol., 1996,53 : 116-117). 2) We have already reported on the peptide based HIV-1 vaccine candidate (Nature Medicine, 1995 1 : 681-685). Using this peptide as a primary immunogen and then boosted by naked DNA injection represent strong augmentation of eliciting humoral and cell-mediated immunity (Vaccine, 1997 in press). 3) The CTL activity against Env was enhanced by co-injection of TCA3 (Immunology, 1996,90 : 1-6), B7-2 (Eur.J.Immunol., 1997 27 : 782-787), IL-12 (J.Immunology, 1997, in press) expressing plasmids. 4) Liposome has a strong adjuvant effect of eliciting antibody and CTL activity (Aids, Res.Hum.Retrovirus.in press).