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Immune response at the fetal-maternal interface during normal gestation

Research Project

Project/Area Number 08045058
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionOsaka Prefecture University

Principal Investigator

YAMATE Jyoji (1998)  Osaka Prefecture University.College of Agriculture, Associate Professor, 農学部, 講師 (50150115)

木曽 康郎 (1996-1997)  大阪府立大学, 農学部, 講師 (10142374)

Co-Investigator(Kenkyū-buntansha) LAMARRE Jonathan  University of Guelph, Ontario Veterinary College, Associate Professor, Associate
OHTA Mitsuaki  Osaka Prefecture University, College of Agriculture, Associate Professor, 農学部, 助教授 (20134504)
ANNE Croy B  University of Guelph Dept. of Biomedical, Professor
CROY B.anne  University of Guelph, Ontario Veterinary College, Professor
CROY B.Anne  University of Guelph Dept. of Biomedical, Professor
河南 有希子  大阪府立大学, 農学部, 助手 (80264810)
山手 丈至  大阪府立大学, 農学部, 講師 (50150115)
LAMARRE Jon  Department of Biomedical Sciences, Unive, 助教授
CROY B. Ann  Department of Biomedical Sciences, Unive, 教授
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsuterine NK cell / apoptosis / habitual abortion / implantation failure / fetal-maternal interface / 顆粒成間膜腺細胞 / 胎盤 / 脱落膜 / MHCクラスII抗原 / 自然流産 / トランスジェニックマウス / パ-フォリン / 顆粒性間膜腺細胞 / 自己免疫疾患マウス / 体外培養法 / マグネット法
Research Abstract

1. The kinetics of uterine NK cells under chemokine-included extracellular matrix and apoptosis-induced factors was clarified by isolating the cells using magnatic cell isolation methods.
2.The differentiation of progenitor cells of uterine NK cells was investigated in vitro by addition of a various kinds of cytokines/chemokines, with special references to cytolysis, and morphology and nature of cytoplamic granules of NK cells.
3.CD3e transgenic mice would be useful for investigation of NK cell function. Using this animal model, interactions of VCAM and ICAM on vascular endothelia to uterine NK cells was examined. Furthermore, the relationship between uterine NK cell differentiation and abortion due to uncomparable MHC class II or cytokine injection was investigated ; this clarified close relationship of habitual abortion to NK cells.
4.Immune response at the fetal-maternal interface is a definite factor for establishment of gestation through placenta formation from fertilized ovary implantation. These studies provided the in vivo data of possible regulation of implantation ; these data lead not only to clarification of pathogenesis of habitual abortion but also to production of animal models of the habitual abortion.
5.In order to continue and promote this project, one professor of Osaka prefecture University went to Argentina (Univ. BuenosAires), U.S.A.(Univ. Harvard, and Univ. California), and Canada (Univ. Guelph). Some professors, Univ. Guelph, were invited to Osaka Prefecture University.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kiso Y. 他5名: "Isolation of granulated metrial gland cells,a uterine form of natural Killer cell,by magnetic immunobeads/pnrticles" J.Reprod. Dev.44. 107-111 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiso Y, 他1名: "Some aspects of granulated metrial gland cells found at the feto-materal interface during successful pregnancy." Reproductive Bidogy Update(Miyamoto H,Manbe N.eds). 327-336 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiso Y. 他5名: "A study of granulated metrial gland cells in the pregnant alymphoplasia (aly/aly) mice." J.Vet.Med.Sci.59. 1137-1141 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsukamoto Y.他8名: "Gicerin,a cell adhesion inclecule,participates in the histogenesis of retina." J.Neurobiol. 33. 769-780 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiso Y. 他3名: "An NK cell subset found in the pregnant murine uterus." JSIR. 7-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Croy B.A. 他5名: "Charterization of that cells that migiate from metrial glands of the pregnant mouse uterus aluring explant culture." J.Reprod.Immunol.32. 241-263 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Croy B. A.他5名: "Characterization of the cells that migrate from metrial glands during culture." J. Reprod. Immunol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukasawa N.他の5名: "Apoptosis of rat uterine NK cells in vitro." J. Reprod. Dev.42・4. 219-224 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 牧田牧子 他4名: "自然流産マウスモデルにおける子宮NK細胞の動態" 第122回日本獣医学会講演要旨集. 77- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 木曽康郎: "妊娠時の子宮NK細胞" 第11回日本生殖免疫学会プログラム・抄録集. 33- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kiso Y. 他3名: "Murime granulated metrial gland cells : An NK cell subset found in the pregnant murine uterus." Proc. Jpan. J. Reprod. Immunol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 日下部 健 他4名: "顆粒性間膜腺細胞のFas/Fas-Lシステムによるアポトーシス" 第123回日本獣医学会講演要旨集. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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