| Project/Area Number |
08045060
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Field |
植物保護
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| Research Institution | Faculty of Agriculture, Tamagawa University |
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Principal Investigator |
WAKABAYASHI Ko Faculty of Agriculture, Tamagawa University, Professor, 農学部, 教授 (00220830)
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| Co-Investigator(Kenkyū-buntansha) |
KOHNO Hitoshi Faculty of Agriculture, Tamagawa University, Professor, 農学部, 教授 (40074356)
SATO Yukiharu Faculty of Agriculture, Tamagawa University, Professor, 農学部, 教授 (40119516)
BRASS Sussane Faculty of Biology, Konstanz University, Lecturer, 生物学部, 講師
NICOLAUS Beate Faculty of Biology, Konstanz University, Assistant professor, 生物学部, 助教授
BOGER Peter Faculty of Biology, Konstanz University, Professor, 生物学部, 教授
SUSSANE Bras コンスタンツ大学, 生物学部, 講師
BEATE Nicola コンスタンツ大学, 生物学部, 助教授
PETER Boger コンスタンツ大学, 生物学部, 教授
BEATE Nicoau コンスタンツ大学, 生物学部, 助教授
小川 人士 玉川大学, 農学部, 講師 (50241047)
BOGER Pecter コンスタンツ大学, 生物学部, 教授
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Protoporphyrinogen-IX oxidase (Protox) / Peroxidizing phytotoxicities / Active oxygen / Destruction of thylakoid membranes / HPLC-Isoluminol chemiluminescence / Protox inhibitors / Molecular design of Protox inhibitors / パーオキシダイザー / ポルフィリン生合成経路 / プロトポルフィリノーゲンIX / プロトポルフィリンIX / クロロプラスト / プロトポルフィリノーゲンオキシダーゼ / 代謝活性化 |
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| Research Abstract |
Phytotoxic diphenyl ethers and cyclic imides induce inhibition of chlorophyll biosynthesis and photooxidative destruction of plant membranes affecting photosynthetic pigment contents. A peroxidizing mechanism of action of the compounds has been proposed already in our precedent study before staring this Grant-In-Aid Research. The immediate physiological response of plants against the peroxidizing compounds is a halt of chlorophyll biosynthesis in chioroplasts by specific inhibition of protoporphyrinogen-IX oxidase. This inhibition is accompanied by an abnormal accumulation of tetrapyrroles. Protoporphyrin-IX, the main tetrapyrrole intermediate accumulated, acts as a photosensitizer in the light and induces radical formation with subsequent destruction of cellular constituents. Decrease of photosynthetic pigments, chlorophylls and carotenoids, quite well correlatesproto porphyrinogen-IX oxidase inhibition by the peroxidizing compounds as well as ethane formation caused by destrucion of thylakoid membranes. This research was conducted to establish and confirm radical reactions involved in active oxygen, to elucidate oxidative destruction of thylakoid membranes.
Results obtained (1-5) are quite useful plant growth regulation, hydrogen production using green algae, and molecular design of medicine controlling active oxygen. (1) In peroxidizing reaction, lipid hydroperoxides were determined using HPLC-System combined with isoluminol-chemiluminescence, thus confirming radical reactions in the process. (2) Photosynthetic electron transport inhibitors alleviated ethane formation. (3) Peroxidizing process proceeds generally in chloroplasts to destroy thylakoid membranes. (4) Through QSAR study of the peroxidizers, newer strong peroxidizers was successfully designed and synthesized. (5) A hydrogen production process using peroxidizer and photosynthetic electron transport inhibitor was proposed.
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