| Project/Area Number |
08309010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | YAMAGUCHI UNIVERSITY (1997)
Okazaki National Research Institutes (1996) |
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Principal Investigator |
MIMURO Mamoru Yamaguchi Univ., Dept.Phys.Biol.& Inform., Prof., 理学部, 教授 (40142004)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Keizo Tokyo Metropol. univ., Dept.Biol., Assoc.Prof., 大学院・理学研究科, 助教授 (80112473)
UEDA Kunihiko Kanazawa Univ., Dept.Bio., Prof.., 理学部, 教授 (60184925)
GO Michiko Nagoya Univ., Dept.Biol.., Prof., 大学院・理学研究科, 教授 (70037290)
FUJIYOSHI Yoshinori Kyoto Univ., Dept.Biophys., Prof., 大学院・理学研究科, 教授 (80142298)
久堀 徹 東京工業大学, 資源化学研究所, 助教授 (40181094)
七田 芳則 京都大学, 理学部, 助教授 (60127090)
緒方 武比古 北里大学, 水産学部, 助教授 (00104521)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 1997: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1996: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | Symmetry of protein assembly / Phylogeny and Evolution / Protein Structure / Structure-Function Relationship / Module Structure / 対称性 / タンパク質 / グロビンファミリー / モジュール / 形 |
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| Research Abstract |
A symmetry structure of protein assemblies is sometimes found in the systems of photosynthesis, an energy conversion system and vision, a signal transduction system. Significance of such symmetry structure was investigated from the following two points ; (1) necessity for function and (phylogeny of proteins having different origins. The major points for analyzes were (1) interaction between prosthetic groups and (2) domain structure and/or module structure of proteins. Analyzes were carried out on photosynthetic reaction center (C_2 symmetry), antenna system (maimly C_3 symmetry), rhodopsin family (C_3 symmetry), and also pseudo C_3 symmetry in ATPase, the last of which might have a significance as well as the symmetry structure.
In the case of photosynthetic antenna system, the symmetry structure is an easy way to increase a number of pigments for light energy collection (or absorption). However, it is very rare that this kind of structure can be assigned to a consequence for the expression of function. For example, phycobiliproteins in cyanobacteria and red algae contain two additional helices at the N terminus of a putative ancestral protein that is assigned to the globin family. Owing to these two helices, phycobiliproteins acquired the symmetry structure different from that of globin families and at the same time, an interaction between chromophores was realized, which was necessity for antennafunction.
In the case of ATPase, breakdown of symmetry structure is shown to be necessity for the expression of function, which was proved by the recent analysis of the three-dimensional structure.
In conclusion, the current subject is concerned with the structure-function relationship of the protein assemblies whose structural data are accumulating very rapidly, and thus it should be continued for further analyzes.
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