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Forensic Studies on Identification of a Hit-and-Run Vehicle

Research Project

Project/Area Number 08407015
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionGunma University School of Medicine

Principal Investigator

KISHI Koichiro  Gunma University School of Medicine, Depatrment of Legal Medicinc, Professor, 医学部, 助教授 (30169841)

Co-Investigator(Kenkyū-buntansha) HOSOMI Osamu  Gunma University School of Medicine, Depatrment of Legal Medicine, Assistant, 医学部, 助手 (30134274)
NAKAJIMA Tamiko  Gunma University School of Medicine, Depatrment of Legal Medicine, Assistant, 医学部, 助手 (40008561)
TAKESHITA Haruo  Gunma University School of Medicine, Department of Legal Medicine, Lecturer, 医学部, 講師 (90292599)
YASUDA Toshihiro  Gunma University School of Medicine, Depatrment of Legal Medicine, Associate Pro, 医学部, 助教授 (80175645)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 1997: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1996: ¥11,100,000 (Direct Cost: ¥11,100,000)
KeywordsPersonal identification / Genetic polymorphism / Deoxyribonuclease / Molecular biology / Human genetics / DNA polymorphism / Enzyme / デオキリシボ核酸分解酵素 / cDNA / STR / DNase I / PCR / 親子鑑定 / 遺伝子構造
Research Abstract

1.Human deoxyribonuclease I (DNase I) polymorphism has been demonstrated to be an extremely useful genetic marker for personal identification in the forensic field. We have developed a novel DNase I-genotyping system using the polymerase chain reaction. The high sensitivity and specificity of this genotyping method allowed us to survey DNase I-polymorphism in small DNA samples.
2.The fifth allele, DNASE1^<**>5, of human DNase I has been discovered in a Japanese population, and consequently, DNase I-polymorphism can be classified into 15 different phenotypes. In addition to these protein polymorphisms, a new polymorphic site due to a C-G substitution has been identified in intron 7 of the human DNase I gene. These findings permit the ability of DNase I-polymorphism in personal identification to be further enhanced.
3.Caucasian and Negroid populations were classified into several DNase I phenotypes. Genetic polymorphism of human DNase I was world-wide. Furthermore, we have clarified the pr … More esence of significant differences in DNase I polymorphism between populations.
4.We have surveyed useful genetic markers for personal identification in human body fluids hitherto. Since salivary and seminal alpha-2-HS glycoprotein (AHSG) was reproducibly phenotypable, AHSG typing could provide an additional discriminate characteristic in the forensic examination of semen and saliva samples. We have determined the DNase I phenotype from both liquid sweat samples and sweat stains on used socks. Therefore, we have shown that the DNase I polymorphism is the genetic marker very effective and indispensable for criminal investigation of sweat-adhered items.
5.We have succeeded in cloning of a full-length cDNA encoding human DNase II which was elucidated by us to exhibit a genetic polymorphism previously. Furthermore, the DNase II gene (DNASE2) could be assigned to 19p13.2-p13.1.This was the first report of the cloning and characterization of a cDNA for mammalian DNase II.
6.We have determined the structure of the respective full-length cDNA encoding rabbit and mouse DNases I.Immunological and biochemical comparison between mammlian DNases I allowed us to construct the molecular evolutional tree of these DNases I.
Considering these findings, DNase I-polymorphism has been confirmed to be the most useful and effective genetic marker suited for personal identification on forensic materials adhered to suspected hit-and-run vehicles. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(120 results)

All Other

All Publications (120 results)

  • [Publications] Nadano, D.: "pH gradient electrophoresis of basic ribonucleases in sealed slab polyacrylamide gels" Electrophoresis. 17(1). 104-106 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nadano, D.: "Purification of mammalian ribonuclease using immobilized human ribonuclease inhibitor" Protein Expression and Purification. 7(2). 167-172 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yazawa, S.: "Histo-blood group Lewis genotyping from human hairs and blood" The Japanese Journal of Human Genetics. 41(1). 177-188 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yazawa, S.: "A search for the Secretor gene nonsense mutation (G42ε to A428)in Japanese nonsecretors" Transfusion. 36(3). 286 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Alpha-2-HS-glycoprotein (AHSG)polymorphism in semen and saliva" Electrophoresis. 17(4). 793-796 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuki, T.: "An applcation of the absorption-elution method to ABO,Lewis,and P1 phenotyping in human teeth" Acta Criminologiae et Medicinae Legalis Japonica. 62(2). 55-58 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Purification of deoxyribonuclesase I (DNaseI)usind two-step chromatography" Analytical Biochemistry. 245(2). 255-257 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aoyama, M.: "DYS19 marker in Japanese populations" International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Population studies of human deoxyribonuclease I polymorphism" Human Heredity. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeshita, H.: "Mouse deoxyyibonuclease I (DNase I)" Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iida, R.: "The fifth allele of the human deoxyribonuclease I (DNase I)polymorphism" Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Rabbit deoxyribonuclease I(DNase I)" Biochemical Journal. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuki, T.: "A case of Ax variant in the ABO system" Acta Criminologiae et Medicinae Legalis Japonica. 62(3). 61-65 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Successful deoxyribonuclease I (DNase I)phenotyping from a small piece of used sock" Electrophoresis. 17(7). 1253-1256 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakajima, T.: "Evaluation of 7 DNA markers (DIS80,HLA-DQ,LDLR GYPS,BGG,D7S8 and GC) in a Japanese population" International Journal of Legal Medicine. 109(1). 47-48 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "A new individualization marker of sweat:deoxyribonuclease I. (DNase I) polymorphism" Journal fo Forensic Sciences. 41(5). 862-864 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iida, R: "Indetification of the nucleotide substitution that generates the fourth polymorphic site in human deoxyribonuclease I (DNase I)" Human Genetics. 98(4). 415-418 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kishi, K.: "Forensic application of genetic polymorphisms detected in human body fluids (urine,semen and blood)" Forensic Science International. 80(1/2). 89-97 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeshita, H.: "The STR loci HumTPO and HumLPL: population genetic data in eight populations" Interhnational Joutnal of Legal Medicine. 110(6). 331-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Activity measurement for deoxyribonucleasesI and II with picogram sensitivity based on DNA/SYBR Green I fluorescence" Anlytical Biochemistry. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hosomi, O.: "Simple purification method of the antiphoshpholipid antibody from normal human plasma" Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Genetic polymorphisms detected in human urine: their application to forensic individualization" The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Molecular cloning of the cDNA encoding human deoxyribonuclease II" The Journal of Biological Chemistry. 273(52). 2610-2616 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsutsumi, S.: "Phenotype 2 of deoxyribonuclease I may be used as a risl factor of gastric cancer" Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeshita, H.: "Detection of the two short tandem repeat loci (HumTPO and HumLPL)in Japanese populations usingdiscontinuous polyacrylamide gel electrophoresis" The Japanese Journal of Legal Medicine. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakajima, T.: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity" Inmunological Investigations. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescencec in situ bybridization analysis" Biochemical and Biophysical Research communications. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Anthropological application of genetic polymorphisms detected in human urine:a review of Japanese studies" Rivista de Antropologia. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 安田 年博: "ヒトの核酸分解酵素、DNase I の診断や治療への応用:ヒトDNase I 遺伝子構造とpolymorphix site の解明" 持田記念財団 研究成果報告書. No.12. 5-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 安田 年博: "ヒトdeoxyribonuclease I 遺伝子における多型部位の同定" DNA 多型. Vol.4. 67-70 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 灘野 大太: "DNA多型によるひき逃げ車両の特定法(微量なDNA資料からのDNase I型判定法)" 交通・予防医学研究財団 研究報告書(第7回)平成6年度助成事業. 95-103 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸 紘一郎: "ヒト核酸分解酵素の遺伝的多型の発見" 日本法医学雑誌. 50(5). 281-291 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸 紘一郎: "DNase Iによる早期癌診断法および治療法の開発:微量なDNA試料からの型判定法" 膵臓病研究財団 第1回研究報告書1993年度. No.1. 63-66 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸 紘一郎: "核酸分解酵素DNase Iの遺伝的多型の発見 尿の利用価値は高い" 医学のあゆみ. 180(10). 658-659 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 灘野 大太: "ヒトのDNase IおよびIIとアポトーシスの関連性:ヒトDNase I遺伝子構造とpolymorphic siteの同定" 総合健康推進財団 研究報告書(第11回)平成6年度助成事業. 111-119 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 灘野 大太: "アポトーシスとエンドヌクレアーゼとの関連性" 臨床薬理の進歩. No.18. 105-110 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 飯田 礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位" DNA多型. Vol.5. 203-206 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 安田 年博: "核酸分解酵素の遺伝的多型を利用した膵肝疾患罹患野ハイリスクグルーブ特定に関する予防医学的研究" 膵臓病研究財団第3回研究報告書. No.3. 1-4 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, T.: "Advances in Tesearch on DNA polymorphism(380-386)" ISFH Hakone Symposium Program Committee(ed.)Toyoshoten,Tokyo, 476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iida, R.: "Advances in Tesearch on DNA polymorphism(321-325)" ISFH Hakone Symposium Program Committee(ed.)Toyoshoten,Tokyo, 476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸 紘一郎: "健康医学ガイド3健康づくり百科・からだと生活と環境・(血液型〔親と子、輸血〕12-13)" 群馬健康医学振興会編、前橋, 251 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸 紘一郎: "現代の法医学改訂第3版(血痕検査391-402)" 永野耐造・若杉長英編、金原出版、東京, 463 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸 紘一郎: "現代の法医学改訂第3版(親子検査417-431)" 永野耐造・若杉長英編、金原出版、東京, 463 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] D.Nadano, T.Yasuda, H.Sawazaki, K., Takeshita, H., K.Kishi: "pH gradient electrophoresis of basic ribonucleases in sealed slab polyacrylamide gels : detection and inhibition of the enzyme activities in the gel." Electrophoresis. 17(2). 104-109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] D.Nadano, T., Yasuda, H.Takeshita, K.Sawazaki, K.Kishi: "Purification of mammalian ribonuclease using immobilized human ribonuclease inhibitor." Protein Expression and Purification. 7 (2). 167-172 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Yazawa, H.Ohkawara, T.Nakajima, O.Hosomi, S.Akamatsu, K.KIshi: "Histo-blood group Lewis genotyping from human hairs and blood." The Japanese Journal of Human Genetics. 41 (1). 177-188 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Yazawa, K.Kishi, S.Akamatsu, H.Ohkawara, T.Seno, Y.Ohkubo: "A search for the Secretor gene nonsense mutation (G428 to A428) in Japanese nonsecretors." Transfusion. 36 (3). 286 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, E.Tsubota, K.Sawazaki, R.Iida, D.Nadano, K.Kishi: "Alpha-2-HS-glycoprotein (AHSG) polymorphism in semen and saliva." Electrophoresis. 17 (4). 793-796 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsuki, K.Kishi: "An application of the absorption-elution method to ABO,Lewis and P_1 phenotyping in human teeth." Acta Criminologiae et Medicinae Legalis Japonica. 62(2). 55-58 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsuki, K.Furukawa, K.Kishi: "A case of Ax variant in the ABO system." Acta Criminologiae et Medicinae Legalis Japonica. 62 (3). 61-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, K.Sawazaki, R.Iida, K.Kishi: "Successful deoxyribonuclease I (DNase I) phenotyping from a small piece of used sock." Electrophoresis. 17 (7). 1253-1256 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nakajima, T.Matsuki, H.Ohkawara, M.Nara, K.Furulawa, K.Kishi: "Evaluation of 7 DNA markers (D1S80, HLA-DQ,LDLR,GYPS,BGG,D7S8 and GC) in a Japanese population." International Journal of Legal Medicine. 109(1). 47-48 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, K.Sawazaki, D.Nadano, R.Iida, S.Miyahara, K.Kishi: "A new individualization marker of sweat : deoxyribonuclease I (DNase I) polymorphism." Journal of Forensic Sciences. 41(5). 862-864 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, H.Takeshita, I.Yuasa, T.Nakajima, K.Kishi: "Identification of the nucleotide substitution that generates the fourth polymorphic site in human deoxyribonuclease I (DNase I)." Human Genetics. 98(4). 415-4158 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Kishi, T.Yasuda: "Forensic application of genetic polymorphisms detected in human body fluids (urine, semen and blood)." Forensic Science International. 80 (1/2). 89-97 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Purification of deoxyribonuclease I (DNase I) using two-step chromatography." Analytical Biochemistry. 245(2). 255-257 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Aoyama, T.Matsuki, K.Kishi, R.Iida, E.Tsubota: "DYS19 marker in Japanese populations." International Journal of Legal Medicine. 110 (2). 112-113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, I.Yuasa, K.Kishi: "Population studies of human deoxyribonuclease I polymorphism." Human Heredity. 47 (3). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, O.Hosomi, Y.Nakasihma, K.Kishi: "Mouse deoxyribonuclease I (DNase I) : biochemical and immunological characterizations, cDNA structure and tissue distribution." Biochemistry and Molecular Biology International. 42 (1). 65-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Rabbit deoxyribonuclease I (DNase I) : purification from urine, immunological and proteochemical characterizations, nucleotide sequence, expression in tissues, relationships to other mammalian DNases I and phylogenetic analysis." Biochemical Journal. 325 (2). 465-473 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, M.Aoyama, E.Tsubota, M.Kobayashi, I.Yuasa, T.Matsuki, K.Koshi: "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism." Electrophoresis. 18(11). 1936-1936 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshita, E.Meyers, B.Brinkmann: "The STR loci HumTPO and HumLPL : population genetic data in eight populations." International Journal of Legal Medicine. 110 (6). 331-333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] O.Hosomi, A.Takeya, T.Yasuda, S.Yazawa, T.Nakajima, T.Kogure, H.Iwai, K.Kishi: "Simple purification method of the antiphospholipid antibody from normal human plasma." Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, K.Kishi: "Genetic polymorphisms detected in human urine : their application to forensic individualization." The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, E.Nakazato, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence." Analytical Biochemistry. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, T.Nakajima, O.Hosomi, Y.Nakashima, K.Kishi: "Molecular cloning of the cDNA encoding human deoxyribonuclease II." The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Tsutsumi, T.Asao, Y.Nagamachi, T.Nakajima, T.Yasuda, K.Kishi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric cancer." Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, O.Hosomi, Y.Nakashima, S.Tsutsumi, K.Kishi: "Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis." The Japanese Journal of Legal Medicine. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, Y.Nakashima, O.Hosomi, H.Takeshita, K.Kishi: "Two novel screening methods for selecting monoclonal antibodies witch specifically inhibit DNase I enzyme activity." Immunological Investigations. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, Y.Nakajima, O.Hosomi, Y.Nakashima, K.Mogi, K.Kishi: "Chromosomal localization of the human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis." Biochemical and Biophysical Research Communications. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, D.Nadano, H.Takeshita, R.Iida, K.Kishi: "Anthropological applocations of genetic polymorphisms detected in human urine : a review of Japanese studies" Rivista di Antropologia. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, T.Nakajima, T.Matsuki, K.Kishi: Molecular analysis of the fourth polymorphic site in human deoxyribonuclease I (DNase I). Advances in Research on DNA Polymorphism (ISFH Hakone Symposium Program Committee, ed.). Toyoshoten, Tokyo, 321-325 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, K.Kishi: The molecular basis for genetic polymorphism of human deoxyribonuclease I.Advances in Research on DNA Polymorphism (ISFH Hakone Symposium Progran Committee, ed.). Toyoshoten, Tokyo, 380-386 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda,T.: "Purification of deoxyribonuclease I(DNase I)using two-step chromatography" Analytical Biochemistry. 245(2). 255-257 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyama,M.: "DYS19 marker in Japanese populations" International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Populations studies of human deoxyribonuclease I polymorphism" Human Heredity. 47(3). 121-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "Mouse deoxyribonuclease I(DNase I)" Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iida,R.: "The fifth allele of the human deoxyribonuclease I(DNase I)polymorphism" Electrophoresis. 18(11). 1936-1939 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Rabbit deoxyribonuclease I(DNase I)" Biochemical Journal. 325(2). 465-473 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "The STR loci HumTPO and HumLPL:population genetic data in eight populations" International Journal of Legal Medicine. 110(6). 331-333 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Activity measurement for deoxyribobnucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence" Analytical Biochemistry. 255(2). 274-276 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hosomi,O.: "Simple porification method of the antiphospholipid antibody from normal human plasma" Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Genetic polymorphisms detected in human urine:their application to forensic individualization" The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Molecular cloning of the cDNA encoding human deoxyribonuclease II" The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsutsumi,S.: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric cancer" Cancer. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "Detection of the two short tandem repeat loci(HumTPO and HumLPL)in Japanese populations using discontinuous polyacrylamide gel electrophoresis" The Japanese Journal of Legal Medicine. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakajima,T.: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity" Immunological Investigations. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Chromosomal localization of a human deoxyribonuclease II gene(DNASE2)to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis" Biochemical and Biophysical Research communications. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Anthropological application of genetic polymorphisms detected in human urine:a review of Japanese studies" Rivista di Antropologia. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 灘野大太: "アポトーシスとエンドヌクレアーゼとの関連性" 臨床薬理の進歩. No.18. 105-110 (1997)

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      1997 Annual Research Report
  • [Publications] 岸紘一郎: "核酸分解酵素DNase Iの遺伝的多型の発見" 医学のあゆみ. 180(10). 658-659 (1997)

    • Related Report
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  • [Publications] 灘野大太: "ヒトのDNaseIおよびIIとアポトーシスとの関連性:ヒトDNaseI遺伝子構造とpolymorphic siteの同定" 総合健康推進財団 研究報告書(第11)平成6年度助成事業. 111-119 (1997)

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  • [Publications] 安田年博: "核酸分解酵素の遺伝的多型を利用した膵肝疾患罹患のハイリスクグループ特定に関する予防医学的研究" 膵臓病研究財団第3回研究報告書. No.3. 1-4 (1997)

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  • [Publications] 飯田礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位" DNA多型. Vol.5. 203-206 (1997)

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  • [Publications] Matsuki,T.: "An application of the adsorption-elution method : ABO,Lowis and P_1 phenotyping in human teeth" Acta Criminological Japonica. 62(2). 55-58 (1996)

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  • [Publications] Matsuki,T.: "A case of Ax variant in the ABO system" Acta Criminological Japonica. 62(3). 61-65 (1996)

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      1996 Annual Research Report
  • [Publications] Nadano,D.: "pH Gradient electrophoresis of basic ribonucleases in sealed slab polyacrylamide gels" Electrophoresis. 17(1). 104-109 (1996)

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      1996 Annual Research Report
  • [Publications] Nadano,D.: "Purification of mammalian ribonuclease using immobilized human ribonuclease inhibitor" Protein Expression and Purification. 7(2). 167-172 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuda,T.: "Alpha-2-HS-glycoprotein (AHSG) polymorphism in semen and saliva" Electrophoresis. 17(4). 793-796 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yazawa,S.: "Histo-blood group Lewis phenotyping from human hairs and blood" Jananese Journal of Human Genetics. 41(1). 177-188 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuda,T.: "Population studies of human deoxyribonuclease I polymorphism" Human Heredity. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 安田年博: "ヒトの核酸分解酵素、DNaseIの診断や治療への応用:ヒトDNaseI遺伝子構造とpolymorphic siteの解明" 持田記念財団 研究成果報告書. 12. 5-11 (1996)

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      1996 Annual Research Report
  • [Publications] 岸紘一郎: "DNaseIによる早期癌診断法および治療法の開発" がん重点研究報告集録 平成7年度(1995). 554-555 (1996)

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      1996 Annual Research Report
  • [Publications] Yasuda,T.: "Anthropological applications of genetic polymorphisms detected in human urine" Rivista di Antropologica. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 安田年博: "ヒトdeoxyribonucleaseI遺伝子における多型部位の同定" DNA多型. 67-70 (1996)

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      1996 Annual Research Report
  • [Publications] 灘野大太: "DNA多型によるひき逃げ車両の特定法(微量なDNA資料からのDNaseI型判定法)" 交通・予防医学研究財団研究報告書(第7回)平成6年度助成事業. 95-103 (1996)

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      1996 Annual Research Report
  • [Publications] Yasuda,T.: "A new individualization marker of sweat : deoxyribonuclease I (DNase1)" Journal of Forensic Sciences. 41(5). 862-864 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kishi,K.: "Forensic applications of genetic polymorphism detected in human body fluids (urine,semen and blood)" Forensic Science International. 80(1/2). 89-97 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakajima,T.: "Evaluation of 7 DNA markers (D1S80,HLA-DQα,LDLR,GYPA,HBGG,D7S8 and GC) in a Japanese population" International Journal of Legal Medicine. 109(1). 47-48 (1996)

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      1996 Annual Research Report
  • [Publications] Yasuda,T.: "Successful deoxyribonulease I (DNase I) phenotyping from a small piece of used sock" Electrophoresis. 17(7). 1253-1256 (1996)

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      1996 Annual Research Report
  • [Publications] Iida,R.: "Identification of the nucleotide substitution that generates the fourth polymorphic site in human deoxyribonuclease I (DNase I)" Human Genetics. 98(4). 415-418 (1996)

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      1996 Annual Research Report
  • [Publications] Yazawa,S.: "A search for the Secretor gene nonsense mutation (G428 to A428) in Japanese nonsecretors" Transfusion. 36(3). 286- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岸紘一郎: "ヒト核酸分解酵素の遺伝的多型の発見" 日本法医学雑誌. 50(5). 281-291 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岸紘一郎: "核酸分解酵素DNaseIの遺伝的多型の発見:尿の利用価値は高い" 医学のあゆみ. 180(7). 1092-1093 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 灘野大太: "ヒトのDNaseIおよびIIとアポトーシスとの関連性:ヒトDNaseI遺伝子構造とpolymorphic siteの同定" 総合健康増進財団 研究報告書(第11)平成6年度助成事業. 111-119 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuda,T.: "Purification of deoxyribonuclease I using two-step chromatography" Analytical Biochemistry. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 岸紘一郎: "DNaseIによる早期癌診断法および治療法の開発:微量なDNA試料からの型判定法" 膵臓病研究財団 第1回研究報告書 1993年度. 63-66 (1996)

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      1996 Annual Research Report
  • [Publications] 岸紘一郎: "現代の法医学 改訂第3版(血痕検査pp.391-402)" 永野耐造・若杉長英編、金原出版、東京, 463 (1990)

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      1996 Annual Research Report
  • [Publications] 岸紘一郎: "現代の法医学 改訂第3版(親子鑑定pp.417-431)" 永野耐造・若杉長英編、金原出版、東京, 463 (1996)

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      1996 Annual Research Report
  • [Publications] 岸紘一郎: "健康医学ガイド3 健康づくり百科-からだと生活と環境-(血液型[親と子、輸血]pp.12-13)" 群馬健康医学振興会編、煥乎堂、前橋, 251 (1996)

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Published: 1996-03-31   Modified: 2016-04-21  

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