Grant-in-Aid for Scientific Research (A)
|Research Institution||Niigata University|
IWAKU Masaaki Niigata University, School of Dentistry, Professor, 歯学部, 教授 (70013927)
YAMAGA Masahiro Niigata University, School of Dentistry, Associate Professor, 歯学部, 助手 (10260548)
AYUKAWA Yukio Niigata University, School of Dentistry, Associate Professor, 歯学部, 助手 (00183145)
KOTA Kohichi Niigata University, School of Dentistry, Associate Professor, 歯学部, 助教授 (90018755)
HOSHINO Etsuro Niigata University, School of Dentistry, Professor, 歯学部, 教授 (90124619)
YOSHIBA Kunihiko Niigata University, Dental Hospital, Assistant Professor, 歯学部附属病院, 助手 (30220718)
|Project Fiscal Year
1996 – 1998
Completed(Fiscal Year 1998)
|Budget Amount *help
¥32,400,000 (Direct Cost : ¥32,400,000)
Fiscal Year 1998 : ¥2,400,000 (Direct Cost : ¥2,400,000)
Fiscal Year 1997 : ¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 1996 : ¥26,500,000 (Direct Cost : ¥26,500,000)
|Keywords||Infected Pulp / Obligate Anaerove / Metoronidazol / alpha-Tricalcium phosphate / Biocompatibility / Antibacterial Drug / Hard Tissure Barrier / Pulp Conservation / 感染歯髄 / 偏性嫌気性菌 / メトロニダゾール / αTCP / 生体親和性 / 抗菌剤 / 被蓋硬組織 / 歯髄保存|
This study was projected for development and clinical application of the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.
Following results were obtained :
1. RFLP Analysis of 16S rDNA from Pseudolamibacter alactolyticus.
In this study, we examined the restriction fragment-length polymorphism(RFLP) of the 16S-rDNA amplified by poymerase chain reaction(PCR) to assess phylogenetic heterogeneity of this bacterial species. In conclusion, these data suggested that P.alactolyticus may not be a phylogenetically homogeneous species.
2. Artificial Bacterial Bioflims and Confocal Laser Scanning Microscopic Analysis.
To elucidate the structure and the function of biofilms, mucoid-type Pseudomonas aeruginosa organisms were cultured in broth media supplied. The biofilm structure was visualized by fluorescent staining and were exposed to ciprofloxacin.
These results indicate that the confocal laser scanning microscopical analysis of artificial biofilms was useful to elucidate bacterial functions in bioflims, and may lead to new quantitative estimation of bactericidal efficacy of natibacterial drugs.
3. Tooth development is regulated by epithelial-mesenchymal interactions mediated by the basement membrane. The expression and localization of laminin-5 were temporally and spatially regulated during mouse tooth development. The temporo-spatial changes in basement membrane composition might be related with tooth morphogenesis and cytodifferentiation.
4. The amount of fluoride release from fluoridated restorative materials, fluoride uptake into enamel and dentine, and effect of fluoride on acid resistance of the tooth structures, were evaluated Fluoride releasing materials was effective for prevention of secondary caries.
5.This work is now in progress