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Studies on the regulation of the expression of circadian clock gene cluster kaiABC by using bioluminescence as a reporter

Research Project

Project/Area Number 08454244
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionNagoya University

Principal Investigator

ISHIURA Masahiro  Nagoya University Graduate School of Science Associate Professor, 大学院・理学研究科, 助教授 (20132730)

Co-Investigator(Kenkyū-buntansha) KONDO Takao  Nagoya University Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10124223)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsCircadian clock gene / Bioluminescence / Gene expression / Cyanobacteria / Luciferase / kaiABC / Promoters / プロモーター / サーカディアンリズム / 生物時計 / 時計遺伝子 / Kaiタンパク質 / Synechococcus sp.PCC 7942 / Sipeihaiecmis. / オペロン / 生物時計突然変異体
Research Abstract

We transferred a genomic DNA library into circadian clock mutants of Synechococcus sp. Strain PCC 7942, selected transformed clones with kanamycin, screened cured clones which showed wildtype phenotype, and recovered the transferred genomic DNA segments from cured clones into E.ccli cells as plasmids by the plasmid rescue procedure. We identified a gene cluster kaiABC which is composed of three genes, kaiA, kaiB, and kaiC and is essential to the clock function (Ishiura et al., 1998. Science 281 : 1519-1523). All the mutations analyzed were missense mutations and mapped to this gene cluster. Homologs of kaiB and kaiC were found in the genomes of archaea, but not found in eukaryotes. Two promoters(PkaiA and PkaiBC), each one in both the upstream regions of kaiA and kaiB, were found, and kaiA and a set of kaiB and kaiC were transcribed as a kaiA mRNA and a kaiBC mRNA, respectively. The expression of both kaiA and kaiBC was circadian and affected by mutations in kaiA, kaiB, and kaiC themselves. KaiA protein enhanced the activity of PkaiBC promoter whereas KaiC protein repressed the activity. Therefore, the essence of the circadian clock in cyanobacteria is the circadian autoregulation of the expression of kaiABC by Kai proteins, KaiA, KaiB, and KaiC.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Tsinoremas,N.F.他: "A pigma factor that modifies the circadian expression of a subred of genes in cyanobactefia" EMBO.J.15. 2488-2495 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kondo,T.,他: "Circadian rhythms in rapidly divicling cyanobacteria" Science. 275. 224-227 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aoki,S.,他: "Circadian rhythms of the cyanobactenium Synechocyotis Sp. Strain Pcc 6803 in the dark." J.Bacteriol. 179. 5751-5755 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kutsuna,S.,他: "A period-extender gene, Pcx, That extends the period of the circadian clock in the cyanobacterium Synechococcus sp, strain Pcc 7942" J.Bacteriol.180. 2167-2174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishiura,M.,他: "Expression of a gene cluster kaiABC as a circadian feedback process in cyansbauterig." Science. 281. 1519-1523 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaki,H.,他: "Physical interactions among circadian clack proteins, Kai A, Kai B, and Kai C" EMBO.J.印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaki, H., Taniguchi, Y., Ishiura, M., and Kondo, T.: "Physical interactions among circadian clock proteins, KaiA,KaiB,and KaiC,in cyanobacteria." EMBO.J.(in press.). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishiura, M., Kutsuna, S., Aoki, S., Iwasaki, H., Andersson, C.R., Tanabe, A., Golden, S., Johnson, C.H., and Kondo, T.: "Expression of a gene cluster kaiABC as a circadianfeedback process in cyanobacteria." Science. 281. 1519-1523 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kutsuna, S., Kondo, T., Aoki, S., and Ishiura, M.: "A Period-extender gene pex thatextends the period of the circadian clock in the cyanobacterium Synechococus sp.strain PCC 7942." J.Bacteriol.180. 2167-2174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aoki, S., Kondo, T., Wada, H., and Ishiura, M.: "Circadian rhythms of the cyanobacterium Synechocysis sp.strain PCC 6803 in the dark." J.Bacteriol.179. 5751-5755 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kondo, T., Mori, T., Lebedeva, N.V., Aoki, S., Ishiura, M., and Golden, S.S.: "Circadian rhythms in rapidly dividing cyanobacteria." Science. 275. 224-227 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsinoremas, N.F., Ishiura, M., Kondo, T., Andersson, C.R., Tanaka, K., Takahashi, H., Johnson, C.H., and Golden, S.S.: "A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria." EMBO J.15. 2488-2495 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kondo,T.: "Circadian rhythms in rapidly dividing cyanobacteria" Science. 275. 224-227 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoki,S.: "Circadian rhythms of the cyanobacterian Synechouytis sp.PCC 6803 in the dar" J.Bacteriol.179. 5751-5755 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kutsuna,S.: "A period-extender gene,pex,that extends the period of the circadian clock in the cyanobacterium Synechococcus sp.strain PCC 7942" J.Bacteriol.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsinoremas et al.: "A sigma factor that rlifies the cricadian expression of a subeet of genes in yandoaecteria" EMBO.J.15. 2488-2495 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kondo,T.et al.: "Circadian bythms in rapidly direiling yanobacteia" Science. 275. 224-227 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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