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Effective Expression of Prolactin Receptor Gene and its Relationship with Milk Production

Research Project

Project/Area Number 08456139
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionThe University of Tokyo

Principal Investigator

SAKAI Senkiti  Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Professor, 大学院・農学生命科学研究所, 教授 (80114487)

Co-Investigator(Kenkyū-buntansha) KOHMOTO Kaoru  Nippon Veterinary and Animal Scinece College, Visiting Professor, 獣医畜産学部, 客員教授 (30011894)
HAYASHI Yoshihiro  Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Professor, 大学院・農学生命科学研究所, 教授 (90092303)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥6,200,000 (Direct Cost: ¥6,200,000)
KeywordsPROLACTIN RECEPTOR / PROLACTIN RECEPTOR GENE / LACTOGENESIS / MAINTENANCE OF LACTATION / MAMMARY GLAND / 分娩前のホルモン変化 / 培養乳腺 / 初乳 / カゼイン / カゼイン遺伝子
Research Abstract

1. Prolactin receptor gene expression at lactogenesis
In the mouse mammary gland, the level of prolactin receptor mRNA decreased about 60% during the first 5 days of pregnancy, and its low levels were maintained until 10 : 00 on day 18 of pregnancy. The level of prolactin receptor mRNA increased about 6 folds during the next 12 h period. The high level was maintained on day zero of lactation. The increase in the prolactin receptor mRNA was caused by the increase in corticosterone in vivo and in vitro. The similar changes were found in the ovariectomized midpregnant mouse mammary gland. It is concluded that the prolactin receptor gene expresses about 12 h before the onset of parturition.
2. Effect of accumulation/removal of milk on prolactin receptor gene expression
Prolactin receptor mRNA in the lactating mouse mammary gland began to decrease 12 h after removal of pups from their mother and its level was the lowest at 18 h. Mice which had been weaned for 24 h were allowed to nurse foster pups for 6 h. Prolactin receptor mRNA was restored during the 6 h-nursing period. The number of prolactin receptor changed in parallel with the level of prolactin receptor mRNA.Prolactin receptor gene expression slowed when the mammary gland accumulated milk maximum. Prolactin receptor mRNA increased in the mammry gland in which milk was suckled by the pups. It is concluded that the main factor controlling the prolactin receptor gene expression is the degree of milk accumulation in the mammary gland.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] S.SAKAI: "Prolactin causes the dissociation of prolactin from plasma membrane receptor in lactatingmouse mammary cell." Endocrine Journal. 43・1. 93-100 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.MIZOGUCHI.J.Y.KIM.S.SAKAI.et al.: "Acute expression of the prolactin receptor gene after ovariectomy in midpregnant mouse mammary gland." Endocrine Journal. 43・5. 537-544 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.MIZOGUCHI.J.Y.KIM.J.ENAMI.et al.: "The regulation of the prolactin receptor gene expression in the mammary gland of early pregnant mouse." Endocrine Journal. 44・1. 53-58 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Y.KIM.Y.MIZOGUCHI.H.YAMAGUCHI.et al.: "Removal of milk by suckling acutely increases the prolactin receptor gene expressionin the lactating mouse mammary gland." Molecular and Cellular Endocrinology.131・1. 31-38 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.MIZOGUCHI.H.YAMAGUCHI.F.AOKI.et al.: "Corticosterone is required for the prolactin receptor gene expression in the late pregnant mouse mammary gland." Molecular and Cellular Endocrinology.132・2. 177-183 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 酒井 仙吉: "泌乳開始のメカニズム" 内分泌・糖尿病 科. 5(3). 267-273 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.SAKAI: "Prolactin causes the dissociation of prolactin from plasma membrane receptor in lactating mouse mammary cell : action of high prolactin concentration." Endocrine Journal. 43 (1). 93-100 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.MIZOGUCHI,J.Y.KIM,T.SASAKI,et al.: "Acute expression of the prolactin receptor gene after ovariectomy in midpregnant mouse mammary gland" Endocrine Journal. 43 (5). 537-544 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.MIZOGUCHI,J.Y.KIM,J.ENAMI,et al.: "The regulation of the prolactin receptor gene expression in the mammary gland of early pregnant mouse." Endocrine Journal. 44 (1). 53-58 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Y.KIM,Y.MIZOGUCHI,H.YAMAGUCHI,et al: "Removal of milk by suckling acutely increases the prolactin receptor gene expression in the lactating mouse mammary gland." Molecular and Cellular Endocrinology. 131 (1). 31-38 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.MIZOGUCHI,H.YAMAGUCHI,F.AOKI et al: "Corticosterone is required for the prolactin receptor gene expression in the late pregnant mouse mammary gland." Molecular and Cellular Endocrinology. 132 (2). 177-183 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.SAKAI: "Prolactin causes the dissociation of prolactin from plasma membrane receptor in lactatingmouse mammary cell." Endocrine Journal. 43・1. 93-100 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.MIZOGUCHl.J.Y.KIM.S.SAKAI.et el.: "Acute expression of the prolactin receptor gene after ovariectomy in midpregnant mouse mammary gland." Endocrine Journal. 43・5. 537-544 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.MIZOGUCHI.J.Y.KIM, J.ENAMI, et al.: "The regulation of the prolactin receptor gene expression in the mammary gland of early pregnant mouse." Endocrine Journal. 44・1. 53-58 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Y.KIM.Y.MIZOGUCHI.H.YAMACUCHl.et al.: "Removal of milk by suckling acutely increases the prolactin receptor gene expression in the lactating mouse mammary gland." Molecular and Cellular Endocrinology. 131・1. 31-38 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.MIZOGUCHI.H.YAMAGUCHI.F.AOKI.et al.: "Corticosterone is required for the prolactin receptor gene expression in the late pregnant mouse mammary gland." Molecular and Cellular Endocrinology. 132・2. 177-183 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hondo,E.,Kurohmaru,M.ら: "Prolactin Receptor Expression in Rat Spermatogenic Cells" Biology of Reproduction. 52巻. 1284-1290 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai,S.: "Processing of Prolactin by Mamnary Cells of the Lactafing Mouse" Animal Science and Technology. 66・9. 810-812 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai,S.: "Prolactin Causes the Dissociation of Prolactin from Plasma Membvane Receptor in lactating Mouse Mammary Cell" Endocrine Journal. 43・1. 93-100 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizoguchi,Y.,Kim,J.Y.ら: "Acute Expression of the Prolactin Receptor Gene ofter Ovariectomy in Midprequant Mouse Mammary Gland" Endocrine Journal. 43・5. 537-544 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizoguchi,Y.,Kim,J.Y.ら: "The regulation of the Prolectin Receptor Gene Expression in the Mammary Gland of Early Pregnant Mouse" Endocrine Journal. 44・1(印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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