| Project/Area Number |
08457090
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
NAKANO Masyasu JICHI MEDICAL SCHOOL,DEPARTMENT OF MICROBIOLOGY,PROFESSOR, 医学部, 教授 (70048958)
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| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Kaoru JICHI MEDICAL SCHOOL,DEPARTMENT OF MICROBIOLOGY,ASSISTANT, 医学部, 助手 (20265242)
KIRIKAE Teruo JICHI MEDICAL SCHOOL,DEPARTMENT OF MICROBIOLOGY,ASSISTANT PROFESSOR, 医学部, 講師 (50192563)
KII Michisato (MURATA Mic) JICHI MEDICAL SCHOOL,EXPERIMENTAL ANIMAL LABORATORY,ASSISTANT PROFESSOR, 医学部, 講師 (00012766)
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | scavenger receptor / scavenger-gene knockout mice / BCG-infection / lipopolysaccharide / nitric oxide production |
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| Research Abstract |
(1) Nitric oxide (NO) production in response to Mycobacterium bovis BCG or bacterial lipopolysaccharide (LPS) by scavenger receptor (ScR) gene knockout (ScR-/-) mice and their parent strain of wild type (ScR+/+) mice were examined. Total nitric production as measured by nitrate excreted daily in urine from ScR-/- mice infected with BCG was not so different from that of BCG-infected ScR+/+ mice. However, the No production in vitro by ScR-/- peritoneal macrophages (MP) taken from the BCG-infected mice was slightly affected in comparison with that of the ScR+/+ MP.NO production by thioglycollate-induced MP infected in vitro with BCG was also examined and the production by ScR-/- MP was less than that by ScR+/+ MP.The affection on NO production by ScR-/- gene was clearly observed on LPS-stimulated MP.The ScR-/- MP responded poorly to smooth-type LPS,Re-chemotype LPS or synthetic lipid A.Expression of inducible NO synthase in the MP to S-LPS was also poor. These findings suggest that ScR have some regulatory role on NO production in response to BCG and LPS.
(2) Cultured ScR-/- MP manifested no significant difference in LPS-induced production of TNF,IL-1 beta and IL-6 compared with those from ScR+/+ mice. However, NO production by MP and NO levels in plasma of ScR-/- mice were clearly different from those of ScR+/+ mice, and seemed to depend on the regulation of background genes of the mice rather than the genes of scavenger receptor.
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