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Novel method to detect T clonality and the antigen specificity

Research Project

Project/Area Number 08457156
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YAMAMOTO Kazuhiko  Medical Institute of Bioregulation, Kyushu University, Professor, 生体防御医学研究所, 教授 (80191394)

Co-Investigator(Kenkyū-buntansha) SHIOKAWA Satoshi  Medical Institute of Bioregulation, Kyushu University, Assistant Professor, 生体防御医学研究所, 助手 (20215940)
EZAKI Ichiko  Medical Institute of Bioregulation, Kyushu University, Research Associate, 生体防御医学研究所, 助手 (40037453)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsT Cell Receptor / PCR / SSCP / T Cell / Collagen induced arthritis
Research Abstract

To identity the existence of antigen-specific T cell responses and to follow the changes of these reactions, it is considered useful to evaluate whether certain T cells clonally accumulate in the lymphocyte population. For this purpose, we have established a novel method to analyze T cell clonality using a combination of reverse transcriptase-polymerase chain reaction of T cell receptor beta chain transcripts and single-strand conformation polymorphism (SSCP). Using this method, we obtained a smear-like pattern of electrophoresed DNA from the heterogeneous T cell population. On the other hand, a single T cell clone exhibits a band in appropriate Vbeta amplification and an accumulatedT cell clone in a heterogeneous lymphocyte population is identified as a band in the background smear pattern. If a lymphocyte population was stimulated by an antigen either in vitro or in vivo, several distinct bands were found to be generated in the background smear. Thus, the dynamic changes of T cell clonal responses could be monitored with this method. Analyzes of several immunological disorders, including autoimmune diseases, malignant disorders, andtransplantation, revealed the involvement of antigen-spesific T cell immune responses in these disorders. Furthermore, taking advantege of the reproduciblemobility of a band of SSCP gel, we are now able tocompare identities of the accumulated T cell Clones in different samples without the need for nucleoptidesequencing of each clone. Such information can thus elucidate the occurrence of uniform or stable immunological reactions in the host and also suggests that these reactions play an important role in vivo. Therefore, taken togeter, the above described novel T cell clonality analysis is considered to be useful in studying the T cell immune responses in various fields of immunology.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(21 results)

All Other

All Publications (21 results)

  • [Publications] Atsushi Nakajima: "Specific clonal T cell accumulation in intestinal lesions of crohn′s disease" The Journal of Immunology. 157. 5683-5688 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Tanaka: "Clonotypic analysis of T cells infiltrating the skin of patients with atopic Dermatitis:Evidence for antigen-driven accumulation of T cells" Human Immunology. 48. 107-113 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazukiko Yamamoto: "Establishment snd application of a novel T cell clonality analysis using single-strand conformation polymorphism of T cell receptor messenger signa" Human Immunology. 48. 23-31 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazukiko Yamamoto: "Significance of antigen-specific T cell clones in collagen diseases:Analysis with a novel T cell clonality evaluation system" Internal Medicine. Vol.3No.4. 242-247 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Torsten A.Hoger: "Accumulation of human T lymhotropic virus (HTLV)-I-specific T cell clones in HTLV-I-associated myelopathy/tropical spastic paraparesis patients" The Journal of Immunology. 159. 2042-2048 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Nkajima, Tatsuhiko Kodama, Yoshio Yazaki, Masakazu Takazoe, Norifumi Saito, Ryoichi Suzuki, Haruo Nishino, Kazuhiko Yamamoto, Jack Silver, and Nobuyuki Matsuhashi: "Spesific clonal T cell accumulation in intestinal lesions of crohn's disease" The Journal of Immunology. 157. 5683-5688 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atushi Tanaka, Hideto Takahama, Tomohiro Kato, Yasuo Kubota, Kiyoshi Kurokawa, Kusuki Nishioka, Masako Mizoguchi, and Kazuhiko Yamamoto: "Clonotypic analysis of T cells infiltrating the skin of patients with atopic Dermatitis : Evidence for antigen-driven accumulation of T cells" Human Immunology. 48. 107-113 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazukiko Yamamoto, Kayo Masuko-Hongou, Atsushi Tanaka, Manae Kurokawa, Torsten Hoeger, Kusuki Nishioka, and Tomohiro Kato: "Establishment and application of a novel T cell clonality analysis using single-strand conformation polymorphism of T cell receptor messenger signals" Human Immunology. 48. 23-31 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuhiko Yamamoto: "Significance of antigen-specific T cell clones in collagen diseases : Analysis with a novel T cell clonality evaluation system" Internal Medicine. 36(4). 242-247 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Torsten A.Hoger, Steven Jacobson, Taketo Kawanashi, Tomohiro Kato, Kusuki Nishioka, and Kazuhiko Yamamoto: "Accumulation of human T lymphotropic virus (HTLV) -I- specific T cell clones in HTLV-I-associated myelopathy/tropical spastic paraparesis patients" The Journal of Immunology. 159. 2042-2048 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Atsushi Nakajima: "Specific clonal T cell accumulation in intestinal lesions of crohn's disease" The Journal of Immunology. 157. 5683-5688 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Atsushi Tanaka: "Clonotypic analysis of T cells infiltrating the skin of patients with atopic Dermatitis:Evidence for antigen-driven accumulation of T cells" Human Immunology. 48. 107-113 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazukiko Yamamoto: "Establishment and application of a novel T cell clonality analysis using single-strand conformation polymorphism of T cell receptor messenger signa" Human Immunology. 48. 23-31 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazukiko Yamamoto: "Significance of antigen-specific T cell clones in collagen diseases:Analysis with a novel T cell clonality evaluation system" Internal Medicine. Vol.36 No.4. 242-247 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Torsten A.Hoger: "Accumulation of human T lymphotropic virus(HTLV)-I-specific T cell clones in HTLV-I-associated myelopathy/tropical spastic paraparesis patients" The Journal of Immunology. 159. 2042-2048 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masuko,K.: "Stable clonal expansion of T cells induced by bone marrow transplantation" Blood. 87. 789-799 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ikeda,Y.: "High frequencies of identical T cell clonotypes in synovial tissues of rheumatoid arthritis patients suggest the occurrence of common antigen-driven immune responses" Arthritis Rheum.39. 446-453 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Komagata,Y.: "Clonal prevalence of T cells infiltrating into the pancreas of prediabetic non obese diabetic mouse" Int.Immunol.8. 807-814 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamoto,K.: "Establishment and application of a novel T cell clonality analysis using single strand conformation polyinorphism of T cell receptor messenger signals" Hum.Immunol.48. 23-31 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka,A.: "Clonotypic analysis of T cells infiltrating the skin of patients with atopic dermatitis:evidence for antigen-driven accum ulation of T cells" Hum.Immunol.48. 107-113 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakajima,A.: "Specific clonal T cell accumulation in intestinal lesions of crohn′s disease" J.Immunol.(in press).

    • Related Report
      1996 Annual Research Report

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Published: 1997-03-31   Modified: 2016-04-21  

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