|Budget Amount *help
¥7,600,000 (Direct Cost : ¥7,600,000)
Fiscal Year 1997 : ¥1,400,000 (Direct Cost : ¥1,400,000)
Fiscal Year 1996 : ¥6,200,000 (Direct Cost : ¥6,200,000)
1. Assay of human cholesterol 7alpha-hydroxylase
Microsomal cholesterol 7alpha-hydroxylase activity was not assayed as an increased measurement when we measured it after solubilizing the microsomes and reconstituting the P450 reaction system ideally ; whereas mitochondrial cholesterol 27-hydroxylase activity was assayed as a markedly increased measurement when we measured it after solubilizing the mitochondria and reconstituting the P450 reaction system ideally.
The results will be published.
2. Cholesterol and vitamin D_3 metabolisms
Cholesterol 27-hydroxylase and vitamin D_3 25-hydroxylase are performed by one common enzyme.
To survey these phenomena, I focused to study enzymes concerning vitamin D_3. First, we purified 25-hydroxyvitamin D_3 1alpha-hydroxylase from rat kidney mitochondria, and published as two papers.
Substrate specificity of the enzyme will be studied in future.
Eto,T.-A.,Nakamura,Y.,Taniguchi,T.,Miyamoto,K.,Nagatomo,J.,Maeda,Y.,Higashi,S.,Okuda,K.-I.and Setoguchi ,T.:Assay of 25-hydroxyvitamin D_3 1α-hydroxylase in rat kidney mitochondria.
Analytical Biochemistry 258:53-58,1998.
Kimura,A.,Kondo,K.-H.,Okuda,K.-I.,Higashi,S.,Suzuki,M.,Kurosawa,T.,Thoma,M.,Inoue,T.,Nishiyori,A.,Yoshino,M.,Kato,H.and Setoguchi,T.:Diagnosis of the first Japanese patient with 3-oxo-Δ^4-steroid 5β-reductase deficiency by use of immunoblot annalysis.
European Journal of Pediatrics 157:386-390,1998.