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Rdgulation Mechanisms of Intracellular Membrane Traffic

Research Project

Project/Area Number 08458190
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe Institute of Physical and Chemical Research (RIKEN) (1997-1998)
The University of Tokyo (1996)

Principal Investigator

NAKANOM Akihiko  RIKEN, Molecular Membrane Biology Lab., Chief Scientist, 生体膜研究室, 主任研究員 (90142140)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
Keywordsprotein secretion / ER-to-Golgi transport / SARI / SEC12 / RERI / RER2 / HRR25 / yeast(S. cerevisiae) / 小胞体-ゴルジ体間輸送 / RER / cis-プレニルトランスフェラーゼ / 酵母(s.cerevisiae) / RST / COPI / COPII / 酵母(S.cerevisiae) / 小胞体(ER) / ゴルジ体 / 小胞輸送
Research Abstract

1. The role of Sar1p in vesicle formation from the endoplasmic reticulum (ER) was investigated in tenus of the regulation of its GTPase cycle. EKS1/HRD3 and SED4 were identified as multicopy suppressors of sar1 ts mutants and suggested to be good candidates of GTPase regulators.
2. The regulation of Sec12p, which is guanine-nucleotide exchange factor of Sar1p, was also studied genetically. A loss-of-function type mutation of HRR25, encoding a yeast casein kinase I, suppressed sec12 ts, suggesting that Hrr25p negatively regulates vesicle budding.
3. The functions of RERI and RER2, genes involved in correct ER localization of Sec 12p, were extensively studied. RERI was shown to encode a Golgi membrane protein, which was essential for retrieval of not only Sec12p but also Sec7lp and Sec63p from the Golgi to the ER. Their transmembrane domains contained Rer1p-dependent retrieval signal. On the other hand. RER2 was demonstrated to code for cis-prenyltransferase, a key enzyme of dolichol synthesis. In addition to the role in protein glycosylation, novel physiological roles of dolichol were suggested from the phenotypes of rer2.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Matsuda, N.: "RMA1, an Arabidopsis thasiana gene whose cDNA suppresses the yeast sec15 mutation, encodes a novel protein with a RING finger motif and an membrane another"Plant Cell Physiol.. 39. 545-554 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi, M.: "Isolation of a tobacco cDNA encoding sar1 GTPall and analysis of its dominant mutations in vesicular traffic using a yeast complementation system"Plant Cell Physiol.. 39. 590-599 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saito, Y.: "Activities of mutant sarl proteins in guanine nucleotide binding, GTP hydrolysis and cell-free transport from the endoplasonic retimlurr to the Gdgiapparatus"J. Biochem.. 124. 816-823 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakamura, N.: "Identification of potential regulatory elements for the framsport of Emp24p"Mol. Biol. Cell. 9. 3493-3503 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato, M.: "The yeast RER2 gene, identified by endoplasmic reticulum protein localization inutations, encodes cis-prenyltransferase, a key enzyme in the dolichol synthesis"Mol. Cell. Biol.. 19. 471-483 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saito, Y.: "Identification of SEC12, SED4, trunceded SEC16 and EKSt/HRD3 as multicopr Sappressors of ts mectants of sarl GTPase"J. Biochem.. 125. 130-137 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Murakami, A.: "The inactive form of a yeast caseinkinase I suppresses the secretory detect of the sec12 metant : I am plication of negative regulation by the H**25 kinase in the vaicle budding from the c********* * ******"J. Biol. Chem.. 274. 3804-3810 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kato, J.: "E.cd : homologue of yeast Rer2. a key en*yme of dolichol synthesis, is essential for the carrier lipid formation in bacterial cell wall synthesis"J. Bacteriol.. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato, M.: "Endoplasmic reticuling localization of Sec12p is achieved by two mechanisms : Rerlp-deperndent retrieval that requires the trans membrane domain and Rerlp-independent retention that involues the cytoplasmic domain"J. Cell Biol.. 134. 279-293 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamanushi, T.: "Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transpart from the endoplasmic reticulum"J. Biochem.. 120. 452-458 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakano, A.: "Identification and characterization of extragenic suppressors of the yeast sec12 ts mutation"J. Biochem.. 120. 642-646 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda, T.: "Arabdopsis gene isolated by a novel method for detecting genetic interation in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase"Platnt Cell. 8. 2079-2091 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato, K.: "Rerlp as common machinery for the endoplasmic reticulum localization of membrance proteins"Proc. Natl. Acad. Sci. U.S.A.. 94. 9693-9698 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyuki Sato, et al.: "Endoplasmic reticulum localization of Sec 12p is achieved by two mechanisms : Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain"J. Cell Biol.. 134. 279-293 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Yamanushi, et al.: "Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum"J. Biochem.. 120. 452-458 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akihiko Nakano: "Identification and characterization of extragenic suppressors of the yeast sec12 ts mutation"J. Biochem.. 120. 642-646 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takashi Ueda, et al.: "An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase"Plant Cell.. 8. 2079-2091 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken Sato, et al.: "Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins"Proc. Natl. Acad. Sci. USA. 94. 9693-9698 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takashi Ueda, et al.: "AtGD12, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor"Gene. 206. 137-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Noriyuki Matsuda, et al.: "RMA1, an Arabidopsis tholiana gene whose CDNA suppresses the yeast secl5 mutation, encodes a novel protein with a RING finger motif and a membrane anchor"Plant Cell Physiol.. 39. 545-554 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masaki Takeuchi, et al.: "Isolation of a tobacco CDNA encoding Sar1 GTPase and analysis of its dominant mutations in vesicular traffic using a yeast complementation system"Plant Cell Physiol.. 39. 590-599 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yumiko Saito, et al.: "Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis and cell-free transport from the endoplasmic reticulum to the Golgi apparatus"J. Biochem.. 124. 816-823 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuhiro Nakamura, et al.: "Identification of potential regulatory elements for the transport of Emp24p"Mol. Biol. Cell.. 9. 3493-3503 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyuki Sato, et al.: "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in the dolichol synthesis"Mol Cell. Biol.. 19. 471-483 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yumiko Saito, et al.: "Identification of SEC12, SED4, truncated SEC16 and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase"J. Biochem.. 125. 130-137 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akiko Murakami, et al.: "The inactive form of a yeast casein kinase I suppresses the secretory defect of the sec 1 2 mutant : Implication of negative regulation by the Hrr25 kinase in the vesicle budding from the endoplasmic reticulum"J Biol. Chem.. 274. 3804-3810 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jun-ichi Kato, et al.: "E. coli homologue of yeast Rer2, a key enzyme of dolichol synthesis, is essential for the carrier lipid formation in bacterial cell wall synthesis"J. Bacteriol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, N.: "RMA1, an Arabidopsis thajiana gene whose cDNA suppresses the yeast sec15 mutation, encodes a novel protein with a RING finger motif and a membrane onthor" Plant Cell Physiol.39. 545-554 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi, M.: "Isolation of a tabacco cDNA encoding Sar1 GTPacl and analysis of its domirant mutations in vesicnlar traffic using a yeast complementation system" Plant Cell Physiol.39. 590-599 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saito, Y.: "Adivities of mutant Sar1 proteins in guanine noccleotide binding, GTP hydrolysis and cell-free transport from the endoplasnic retiunluin to the Golgiappardpus" J.Biochem.124. 816-823 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura, N.: "Identification of potential regulatory elements for the fromsport of Erge 24p." Mol.Biol.Cell. 9. 3493-3503 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato, M.: "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in the dolichol synlhesis" Mol.Cell.Biol.19. 471-483 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saito, Y.: "Identification of SEC12, SED4, truncated SEC16 and EKSl/HRD3 as multicopy sappressors of ts mutants of Sar1 GTPasc" J.Biochem.125. 130-137 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Murakami, A.: "The inactive from of a yeast casainkinase I suppresscs the secretory defect of the ses12 mutant:Implication of negative regulation by the Hrr25 kinase in the vencle budding from the endoplasme sction" J.Biol.Chem.274. 3804-3810 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] kato, J.: "E.cd:homologue of yeast Rer2, a key enzymcof dolichol synthesis, is essential for the carrier lipid formation in bacterial cell wall synthesis" J.Bacteriol.(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato,K.: "Rerlp as common machinery for the endoplasmic reticulum localization of membrane proteins" Proc.Natl.Acad.Sci.U.S.A.94. 9693-9698 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sato,M.: "Endoplasmic reticulun localization of Sec12p is achieved by two mechanisms:Rer1p-dependent retricual that requires the trans membrane domain and Rer1p-independent retention that involves the cytoplasmic domain" J.Cell Biol.134. 279-293 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamanushi,T.: "Characterization of yeast sar1 temperature-sensitive mutants,which are defective in protein transport from the endoplasmic reticutum" J.Biochem.120. 452-458 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakano,A.: "Identification and characterization of extragenic suppressors of the yeast sec12 ts mutation" J.Biochem.120. 642-646 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ueda,T.: "Arabdopsis gene isolated by a novel method for detecting genetic interation in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase" Plant Cell. 8. 2079-2091 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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