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Optimization mechanism of electron transfer proteins

Research Project

Project/Area Number 08458214
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOkazaki National Institute

Principal Investigator

ITOH Shigeru  National Institute for Basic Biology, Associate Professor, 基礎生物学研究所, 助教授 (40108634)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsprotein / electron transfer / photosynthesis / chlorophyll / Quinone / photochemical reaction / photosynthetic bacteria / cyanobacteria / 反応中心 / 蛋白質 / 進化
Research Abstract

Optimization mechanisms of intra-protein electron transfer were studied in four systems below. (1) plant photosystems I and II reaction centers. (2) reaction center complexes of green sulfur bacteria. (3) A new type of photosynthetic purple bacteria that uses Zn-containing bacteriochlorophylls. (4) a new type of cyanobacteria that uses chlorophyll d which absorbs far-red light, In all the photosynthetic organisms thus far known, chlorophylls are known to be essential. We, however, showed (1) proteins function to optimize the reaction environment of chlorophylls through quinone-exchange studies in the plant photosystem I and H reaction centers, (2) Zn- bacteriochlorophyll can replace the function of Mg-bacteriochlorophyll in vivo, and (3) chlorophyll d also supports the oxygenic photosynthesis as efficient as that by chlorophyll a. These results indicate that natural photosynthetic systems have more varieties than ever estimated and that the photosynthesis with new types of chlorophylls were enabled by a minor tuning of the protein structure around chlorophylls or quinones inside the reaction center protein.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Iwaki,M.: "△G_0 dependence of the electron transfer rate in the photosynthetic reaction center of plant photosystem I : Natural optimization of reaction between chlorophyll a (A0) and quinone." J.Phys.Chem.100. 10802-10809 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh,S.: "Dibromothymoquinone (DBMIB) replaces the function of QA at 77K in the isolated Photosystem II Reaction Center (D1-D2-cytochrome b559) complex" Plant and Cell Physiology. 37. 833-839 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saeki,K.: "Site-specific mutagenesis of Rhodobacter capsulatus ferredoxin I, FdxN, that functions in nitrogen fixation-role of extra residues." J.Biol.Chem.271. 31399-31406 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wakao,N.: "Discovery of natural photosynthesis using Zn-containing bacteriochlorophyll in an aerobic bacterium acidophilium rubrum." Plant Cell Physiol.37. 889-893 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Dzuba,S.: "Electron spin echo of spin-polarized radical pairs in the intact and quinone-reconstituted plant photosystem I reaction centers." Chem.Phys.Letters. 264. 238-244 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomo,T.: "Topology of pigments in the isolated photosystem II reaction center studied by selective extraction." Biochimica Biophysica Acta. 1321. 21-30 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohoka,H.: "Viscosity dependence of the electron transfer rate from bound cytochrome c to P840 in the photosynthetic reaction center of a green sulfur bacterium Chlorobium tepidum." Biochemistry. 36. 9267-9272 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara,H.: "The distance between P680 and QA in Photosystem II determined by ESEEM spectrocscopy." Biochimica Biophysica Acta. 1322. 77-85 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh,S.: "Accumulation of Fe, Cr and Ni metals inside cells of acidophilic bacterium Acidiphilium rubrum that produced Zn containing bacteriochlorophyll a." Plant and Cell Physiology. 39. 740-744 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwaki,M.: "Spin-polarized radical pair in Photosystem I reaction center that contains different quinones and fluorenones as the secondary electron acceptor." J.Phys.Chem.102. 10440-10445 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hu,Q.: "A photosystem I reaction center driven by chlorophyll d in oxygenic phtosynthesis." Proc.Natl.Acad.Sci.USA.95. 13319-13323 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwaki, M.: "DELTAG_O dependence of the electron transfer rate in the photosynthetic reaction center of plant photosystem I : Natural optimization of reaction between chlorophyll a (AO) and quinone." J.Phys.Chem.100. 10802-10809 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh, S.: "Dibromothymoquinone (DBMIB) replaces the function of QA at 77K in the isolated Photosystem II Reaction Center (D1-D2-cytochrome b559) complex." Plant and Cell Physiology. 37. 833-839 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saeki, K.: "Site-specific mutagenesis of Phodobacter capsulatus ferredoxin I,FdxN, that functions in nitrogen fixation-role of extra residues." J.Biol.Chem.271. 31399-31406 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wakao, N.: "Discovery of natural photosynthesis using Zn-containing bacteriochlorophyll in an aerobic bacterium acidophilium rubrum." Plant Cell Physiol. 37. 889-893 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Dzuba, S.: "Electron spin echo of spin-polarized radical pairs in the intact and quinone-reconstituted plant photosystem I reaction centers." Chem.Phys.Letters. 264. 238-244 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomo, T.: "Topology of pigments in the isolated photosystem II reaction center studied by selective extraction." Biochimica Biophysica Acta. 1321. 21-30 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohoka, H.: "Viscosity dependence of the electron transfer rate from bound cytochrome c to P840 in the photosynthetic reaction center of a green sulfur bacterium Chlorobium tepidum." Biochemistry. 36. 9267-9272 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara, H.: "The distance between P680 and QA in Photosystem II determined by ESEEM spectrocscopy." Biochimica Biophysica Acta. 1322. 77-85 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh, S.: "Accumulation of Fe, Cr and Ni metals inside cells of acidophilic bacterium Acidiphilium rubrum that produced Zn containing bacteriochlorophyll a." Plant and Cell Physiology. 39. 740-744 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwaki, M.: "Spin-polarized radical pair in Photosystem I reaction center that contains different quinones and fluorenones as the secondary electron acceptor." J.Phys.Chem.102. 10440-10445 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hu, Q.: "A photosystem I reaction center driven by chlorophyll d in oxygenic phtosynthesis" Proc.Natl.Acad.Sci.USA.95. 13319-13323 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh, S.: Electron transfer in plant photosystem I photosynthetic reaction center containing artificial molecules. In New Challenges in Organic Electrochemistry (edit.T.Osa). Goprdon and Breach Sci.Pub., 265-277 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh, S.: A novel phtosystem I-type reaction center from an oxygenic prokaryote Acaryochloris marina that contains chlorophyll d. In Phototrophic prokaryotes edited by G.A.Peschek, W.Loffelhardt and G.Schmetterer. Kluwer Academic/Plenum Publishers, New York, (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤 繁: "地球を変えた光合成" 日本物理学会誌. 53(2). 100-106 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Itoh,S.et al.: "Accumulation of Fe,Cr and Ni metals inside cells of acidophilic bacterium Acidiphilium rubrum that produced Zn containing bacteriochlorophyll a." Plant and Cell Physiology. (in press.). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohoka,H.et al.: "Viscosity dependence of the electron transfer rate from bound cytochrome c to P840 in the photosynthetic reaction center of a green sulfur bacterium Chlorobium tepidum." Biochemistry. 36. 9267-9272 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomo,T.et al.: "Topology of pigments in the isolated photosystem II reaction center studied by selective extraction." Biochimica Biophysica Acta. 1321. 21-30 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Dzuba,S.A.et al.: "Electron spin echo of spin-polarized radical pairs in the intact and quinone-reconstituted plant photosystem I reaction centers." Chemical Physics Letters. 264. 238-244 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hara,H.et al.: "The distance between P680 and Q_A in Photosystem II determined by ESEEM" Biochimica Biophysica Acta. 1322. 77-85 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Baba,K.et al.: "Photoinhibition of photosystem I electron transfer activity in isolated photosystem I preparations with different chlorophyll contents." Photosyntesis Research. 347. 121-130 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iwaki,M.et al.: "ΔG^0 dependence of the electron transfer rate in photosynthetic reaction center of plant photosystem I : Natural optimization of reaction between chlorophyll a (A_0) and quinone." Journal of Physical Chemistry. 100. 10802-10809 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Itoh,S.et al.: "Stabilization of P680^+ at 77 K in the Quinone-reconstituted Photosystem II Reaction Center (D1-D2-cytochrome b559) complex." Plant and Cell Physiology. 37. 833-839 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wakao,N.et al.: "Discovery of natural photosynthesis using Zn-containing bacteriochlorophyll in an aerobic bacterium Acidiphilium rubrum." Plant and Cell Physiology. 37. 889-893 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saeki,K.et al.: "Site-specific mutagenesis of Rhodobacter capsulatus Ferredoxin I,FdxN,that functions in nitrogen fixation." Journal of Biological Chemistry. 271. 31399-31406 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Itoh,S.and Iwaki,M.: "Electron transfer in plant photosystem I photosynthetic reaction center containing artificial molecules." in "New challenges in Organic Chemistry" (Ed.by Osa,T.,Gordon and Breach Science Publishers,Amsterdam,Netherlands). 265-277 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wakao,N.: "Discovery of natural photosynthesis using Zn-containing bacteriochlorophyll in an aerobic bacterium Acidophilium rublum." Plant and Cell Physiology. 37. 889-893 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saeki,K.: "Site-specific mutagenesis of Rhodobacter capsulatus ferredoxinI,FdxN,that functions in nitrogen fixation." J.Biological Chemistry. 271. 31399-31406 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Dzuba,S.A.: "Electron spin echo of spin-polarizedradicalpairs in the intact and quinone-reconstitutedplant PS I reactin centers." Chemical Physics Letters. 264. 238-244 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iwaki,M: "ΔG_O dependence of the electron transfer rate in photosynthetic reaction center of plant photosystem I." J.Phys.Chem.100. 10802-10809 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Itoh,S.: "Stabilization of P680+ at 77 K in the quinone-reconstitutedphotosystem II reaction center complex." Plant and Cell Physiology. 37. 833-839 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Baba,K.: "Photoinhibition of photosystem I electron transfer activity in isolated PS I preparations with different chlorophyll contents." Photosynthesis Research. 47. 121-130 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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