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The study of the mechanism of myotube formation in the course of skeletal myoblast differentiation.

Research Project

Project/Area Number 08458232
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

KIM Jeman  Kyoto Pharmaceutical University, Department of Cell Biology, Professor., 薬学部, 教授 (30029805)

Co-Investigator(Kenkyū-buntansha) HIRAYAMA Etsuko  Kyoto Pharmaceutical University, Department of Cell Biology, Assistant., 薬学部, 助手 (10247786)
ADACHI Toshiyuki  Kyoto Pharmaceutical University, Department of Cell Biology, Assistant., 薬学部, 助手 (40202634)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsmyogenesis / myotube formation / cell fusion / HVJ / myogenin / cell membrane / myoblast / striated structure / 骨格筋 / 人工筋管 / 細胞分化 / 分化プログラム / 横紋形成 / タンパク質のリン酸化 / Myogenin
Research Abstract

For investigating the mechanism of skeletal myoblastdifferentiation, especiallymyoblast fusion, we established QM-RSV cells, quail myoblasts transformed with a temperature-sensitive mutant of Rous sarcoma virus. QM-RSV cells proliferate at 35.5゚C, a permissive temperature for RSV, but differentiateat 41゚C, anonpermissive temperature, with the formation of multinucleated myotubesand myofibrilbundles appear in myotubes with maturation.
As a good tool for analysis, we had prepared a monoclonal antibody, H-145 that inhibits myotube formation of QM-RSV cells. The dynamic distribution of H-145 antigen during differentiationof QM-RSV cells was examined by indirect-immunofluorencent staining using H-145. The results suggest that quantitative expression of H-145 antigen on the cell surface is a prerequisite for myoblast fusion and it related directly to a step of membrane fusion upon differentiation of QM-RSV cells.
On the other hand, detailed experiments of a muscle regulatory factor, myogenin showed that myogenin expression is one of the indispensable conditions for the acquisition of commitment to differentiation, and is regulatedby tyrosinephosphorylation and dephosphorylation of some protein(s) in QM-RSV cells. Further, myogenin was continuously expressed in myotubes not having striated structures comprised of myofibrils, but not in myotubes having the structures, These results also suggests that myogenin is not only requiredfor early steps during differentiation but also maturation steps of my otubes.
In addition, when the characteristics of cell membranes in myoblast differentiation was investigated by HVJ(Sendai virus)-mediatedcells fusion reaction, it was suggested that the cell membranes of myoblasts changed to a fusion-capable state for myotube formation during differentiation.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report

Research Products

(16 results)

All Other

All Publications

  • [Publications] Hidenori Hase, et al.: "Further investigation of some inhibitors on myogenic differentiation : Mechanism of inhibition with HMBA on quail myoblasts transformed with Rous sarcoma virus." Cell Structure and Function. 21. 17-25 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Noriko Inoue-Hyodo, et al.: "Dynamic distribution of an antigen involved in differentiation of quail myoblasts transformed with Rous sarcoma virus : Requirement of its quantitative expression on the cell surface for myoblast fusion." Cell Structure and Function. 21. 515-524 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Etsuko Hirayama, et al.: "Myogenin expression is necessary for commitment to differentiation and is closely related to src tyrosine kinase activity in quail myoblasts transformed with Rous sarcoma virus." European Journal of Cell Biology. 72. 133-141 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshiyuki Adachi, et al.: "Aggregation independent of N-cadherin and neural cell adhesion molecule on quail myoblasts transformed with temperature-sensitive Rous sarcoma virus." Cell Adhesion and Communication. 6. 39-50 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akiko Isobe, et al.: "Characterization of myogenin expression in myotubes derived from quail myoblasts transformed with a temperature sensitive mutant of Rous sarcoma virus." Cell Structure and Function. 23. 57-67 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Etsuko Hirayama, et al.: "Mouse C2 myoblast cells resist HVJ (Sendai virus)-mediated cell fusion in the proliferating stage but become capable of fusion after differentiation." Differentiation. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hidenori Hase, et al.: "Further investigation of some inhibitors on myogenic differentiation : Mechanism of inhibition with HMBA on quail myoblasts transformed with Rous sarcoma virus." Cell Structure and Function. 21. 17-25 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Noriko Inoue-Hyodo, et al.: "Dynamic distribution of an antigen involved in differentiation of quail myoblasts transformed with Rous sarcoma virus : Requirement of its quantitative expression on the cell surface for myoblast fusion." Cell Structure and Function. 21. 515-524 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Etsuko Hirayama, et al.: "Myogenin expression is necessary for commitment to differentiation and is closely related to src tyrosine kinase activity in quail myoblasts transformed with Rous sarcoma virus." European Journal of Cell Biology. 72. 133-141 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshiyuki Adachi, et al.: "Aggregation independent of N-cadherin and neural cell adhesion molecule on quail myoblasts transformed with temperature-sensitive Rous sarcoma virus." Cell Adhesion and Communication. 6. 39-50 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akiko Isobe, et al.: "Characterization of myogenin expression in myotubes derived from quailmyoblaststransformed with a temperature sensitivemutant of Rous sarcoma virus." Cell Structure and Function. 23. 57-67 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Etsuko Hirayama, et al: "Mouse C2 myoblast cells resist HVJ (Sendai virus)-mediated cell fusion in the proliferating stage but become capable of fusion after differentiation." Differentiation. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Etsuko Hirayama, et al.: "Mouse C2 myoblast cells resist HVJ (Sendai virus) -mediated cell fusion in the proliferating stage but become capable of fusion after differentiation." Differentiation. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hidenori Hase,et al.: "Further Investigation of Some Inhibitors on Myogenic Differentiation : Mechanism of Inhibition with HMBA on Quail Myoblasts Transformed with Rous Sarcoma Virus" CELL STRUCTURE AND FUNCTION. 21. 17-25 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noriko Inoue-Hyodo,et al.: "Dynamic Distribution of an Antigen Involved in Differentiation of Quail Myoblasts Transformed with Rous Sarcoma Virus : I.Requirement of its Quantitative Expression on the Cell Surface for Myoblast Fusion" CELL STRUCTURE AND FUNCTION. 21. 515-524 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Etsuko Hirayama,et al.: "Myogenin expression is necessary for commitment to differentiation and is closely related to src tyrosine kinase activity in quail myoblasts transformed with Rous sarcoma virus" European Journal of Cell Biology. 72. 133-141 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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