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Isolation of the fetal brain-enriched cDNA clones and analysis of their corresponding mRNAs expressed in human brain tumors.

Research Project

Project/Area Number 08458243
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionNiigata University

Principal Investigator

USUI Hiroshi  Brain Res.Inst., Niigata Univ., Research Associate, 脳研究所, 助手 (20192510)

Co-Investigator(Kenkyū-buntansha) WASHIYAMA Kazuo  Brain Res.Inst., Niigata Univ., Associate Professor, 脳研究所, 助教授 (00183715)
KUMANISHI Toshiro  Brain Res.Inst., Niigata Univ., Professor, 脳研究所, 教授 (40018601)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordsbrain tumor / brain development / gene expression / cDNA cloning / tumor marker / pituitary adenoma
Research Abstract

We isolated the fetal brain-enriched cDNA clones whose corresponding mRNAs were expressed preferentially in the fetal brain during the brain development, and analyzed the mRNA expression in the human brain tumors. Using the differential screening and cDNA subtraction procedures, we identified 22 distinct fetal brain-enriched cDNA clones which included beta-tubulin Mbeta5, beta-tubulin Mbeta2, alpha-tubulin Malpha1, thymosin beta10, stathmin, neuronatin, ferritin L chain, alpha-internexin, amphoterin, PI-kinase type IIbeta, SAPAP-4, MARKS,MAZ,and MN1. We also isolated the full length cDNA of 3 novel clones (FBE5, FBE88, FBE254) and analyzed their coding proteins. The clone FBE88 revealed to encode a novel transcriptional regulatory protein which belonged to Polycomb-group (PcG) genes and showed some similarity to a Drosophila tumor suppressor gene, lethal (3) malignant brain tumor. Among the results of the expression analyzes of the mRNAs corresponding to the isolated fetal brain-enriched clones in various human tumors, the expression pattern of the neuronatin mRNA seemed noteworthy. The neuronatin mRNA was expressed selectively in human pituitary adenomas among various human tumor tissues and abundantly in almost all non-functioning adenomas which express no functional hormones, suggesting the possible utility of the neuronatin as a new tumor marker of the human pituitary adenomas.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Usui, H.: "cDNA cloning and mRNA expression analysis of the human neuronatin:high level expression in human pituitary gland and pituitary adenomas" J.Mol.Neurosci.9. 55-60 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Feng, X-L.: "Postnatal developmental changes of NSE and NNE mRNA expression in rat pineal gland" J.Pineal Res.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsumanuma, I.: "Infrequent mutation of waf1/p21 gene,a CDK inhibitor gene,in brain tumors" Neurol.Med.Chir.37. 150-157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 馮雪蓮: "ラット松果体におけるNSE,NNE mRNAの発現:in situ hubridizationによる解析" 新潟医誌. 111. 156-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 張淑靖: "グリオーマ細胞株におけるがん抑制遺伝子変化の解析" 新潟医誌. 111. 47-56 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Usui, H.: "Isolation of cDNA clones of the rat mRNAs expressed preferentially in the prenatal stages of brain development" Dev.Brain Res.97. 185-193 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Washiyama, K.: "Neurotrophin and neurotrophin receptor proteins in medulloblastomas and other primitive neuroectodermal tumors of the pediatric central nervous system" Am.J.Pathol.148. 929-940 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koga, H.: "Analysis of p53 gene mutations in human glioma cell lines" Brain Tumor Pathol.13. 1-10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kumanishi, T.: "Primary malignant lymphoma of the brain:demonstration of frequent p16 and p15 gene deletions" Jpn.J.Cancer Res.87. 691-695 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang, S.: "A comparative study of glioma cell lines for p16,p15,p53 and p21 gene alteration" Jpn.J.Cancer Res.87. 900-907 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kilduff, T.S.: "Molecular Regulation of Arousal States" CRC Press(New York), 15 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 熊西 敏郎: "現代病理学体系" 中山書店, 17 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Usui, H.: "cDNA cloning and mRNA expression analysis of the human neuronatin : high level expression in human pituitary gland and pituitary adenomas" J.Mol.Neurosci. 9. 55-60 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Feng, X-L.: "Postnatal developmental changes of NSE and NNE mRNA expression in rat pineal gland" J.Pineal Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kilduff, T.S.: "Isolation and identification of specific transcripts by subtractive hybridization" in : Molecular Regulation of Arousal State. CRC Press. 103-118 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsumanuma, I.: "Infrequent mutation of Waf1/p21 gene, a CDK inhibitor gene, in brain tumors" Neurol.Med.Chir.37. 150-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang, S.: "Analysis of the mutations of tumor suppressor genes in human glioma cell lines (Japanese)" Niigata Med.J.111. 47-56 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Feng, X-L.: "In situ hybridization analysis of NSE and NNE mRNAs in rat pineal gland (Japanese)" Niigata Med.J.111. 159-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Usui, H.: "Isolation of cDNA clones of the rat mRNAs expressed preferentially in the prenatal stages of brain development" Dev.Brain Res. 97. 185-193 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Washiyama, K.: "Neurotrophin and neurotrophin receptor proteins in medulloblastomas and other primitive neuroectodermal tumors of the pediatric central nervous system" Am.J.Pathol.148. 929-940 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Koga, H.: "Analysis of p53 gene mutations in human glioma cell lines" Brain Tumor Pathol. 13. 1-10 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kumanishi, T.: "Primary malignant lymphoma of the brain : demonstration of frequent p16 and p15 gene deletions" Jpn.J.Cancer Res. 87. 691-695 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zhang, S.: "A comparative study of glioma cell lines for p16, p15, p53 and p21 gene alteration" Jpn.J.Cancer Res. 87. 900-907 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kumanishi, T.: "Tumor suppressor genes and human brain tumors (Japanese)" in : Current Encyclopedia of Patholgy, Nakayama Publishing Comp.257-273 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Usui,H.et al: "cDNA cloning and mRNA expression analysis of the human neuronatian" J.Mol Neurosci.9. 55-60 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Feng,X-L.et al: "Postnatal developmental changes of NSE and NNE mRNA expression in rat pineal gland" J.Pineal Res. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsumanuma,I.et al: "Infrequent mutation of waf1/p21 gene, a CDK inhibitor gene, in brain tumors" Neurol Med Chir. 37. 150-157 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagai,S.et al: "Differential screening analysis of the two astrocytic tumor cell lines exhibiting different biological characters" Brain Pathol. 7(4). 1088-1088 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 張淑靖・熊西敏郎: "グリオーマ細胞におけるがん抑制遺伝子変化の解析" 新潟医誌. 11(1). 47-56 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kilduff,T.S.et al.: "Molecular Regulation of Arousal States" CRC Press(New York), 15 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Usui,H.: "Isolation of cDNA clones of the rat mRNAs expressed preferentially in the prenatal stages of brain development" Dev.Brain Res.97. 185-193 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagai,S.: "Identification of differentially expressed mRNAs between two astrocytic tumor cell lines derived from the same patient" Human Cell. 9. 89- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Washiyama,K.: "Neurotrophin and neurotrophin receptor proteins in medulloblastomas and other primitive neuroectodermal tumors of the pediatric central nervous system" Am.J.Pathol.148. 929-940 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koga,H.: "Analysis of p53 gene mutations in human glioma cell lines" Brain Tumor Pathol.13. 1-10 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kumanishi,T.: "Primary malignant lymphoma of the brain : Demonstration of frequent p16 and p15 gene deletions" Jpn.J.Cancer Res.87. 691-695 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zhang,S.: "A comparative study of glioma cell lines for p16,p15,p53 and p21 gene alteration" Jpn.J.Cancer Res.87. 900-907 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kilduff,T.S.: "Molecular Regulation of Conscious State" CRC Press (in press), (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 熊西敏郎: "現代病理学体系" 中山書店, 17 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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