| Project/Area Number |
08458261
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neuroscience in general
|
|---|
| Research Institution | KEIO UNIVERSITY |
|---|
Principal Investigator |
KAWANO Hitoshi KEIO UNIV., SCHOOL OF MEDICINE,DEPT.OF ANATOMY,ASSOCIATE PROFESSOR, 医学部, 講師 (20161341)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Kosei DIVISION OF CELL BIOLOGY,NARA ISTITUTE OF SCIENCE AND TECHNOLOGY ASSISTANT, 助手 (90206946)
OHYAMA Kyoji KEIO UNIV., SCHOOL OF MEDICINE,DEPT.OF ANATOMY,ASSISTANT, 医学部, 助手 (00255423)
NOGAMI Haruo KEIO UNIV., SCHOOL OF MEDICINE,DEPT.OF ANATOMY,ASSOCIATE PROFESSOR, 医学部, 講師 (30119838)
KAWAMURA Koki KEIO UNIV., SCHOOL OF MEDICINE,DEPT.OF ANATOMY,PROFESSOR, 医学部, 教授 (40048286)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | mouse / midbrain / whole embryo culture / development / L1 / phosphacan / NCAM-H / アンチセンスDNA / 中脳黒質 / ドーパミンニューロン / 放射状グリア / テネイシン / チロシン水酸化酵素 |
|---|
| Research Abstract |
In spite of the great advance of the gene technology in recent years, the mechanism of mammalian brain development is not fully known. One of the reasons is the difficulty in observing and manipulating mammalian embryos which develop in the mother's uterus. In the present study, some experimental manupilations were made to the embryonic mouse brain by using the whole embryo culture system to clarify the mechanisms of the brain development. We have already studied the in vivo development of the mouse midbrain and obtained the data which indicate that molecular interactions between neural cell adhesion molecules, L1 and a brain-specific chondroitin sulfate proteoglycan, phosphacan, are involved in the migration of mesencephalic dopamine-containing neurons (Kawano et al., 1997 ; Ohyama et al., 1998 in press). Since L1 have been reported to bind phosphacan in vivo, molecular interaction between the two molecules may play important roles in the migration of dopamine neurons. Based on these observation, we made microinjection of an enzyme which degrades chondrotin sulfate and antibody ageinst L1 into the ventricle of E13 mouse embryos using the whole embryo culture system, and exmined the brain histologically. This system will be a useful tool to study the developmental mechanism of the mammalian brain.
|
