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Higher Order Structure of Long DNA Molecules : Controlling the Structure Based on the Knoledge of the Mechanism in the Transition

Research Project

Project/Area Number 08459013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionNagoya University

Principal Investigator

YOSHIKAWA Ken-ichi  Nagoya University, Graduate School of Human Infomatics, Professor, 大学院人間情報学研究科, 教授 (80110823)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsLong DNA Chain / First Order Phase Transition / Single Chain Observation / Fruorescence Microscopy / Gene Transfection / Phase Separation in a Single Molecule / Coil-Globule Transition / Monte Carle Simulation / 履歴現象
Research Abstract

In the present research, we have fownd that individual single duplex DNA chains undergoes large discrete phase transition between elongated coil and compacted globule (coil-globule transition), based on the single chain observation by use of fluorescence microscopy.
Whereas ensemble of DNA has the characteristics of diffuse or continuous transition. From single chain observation, we found that the first order phase transition is induced by various kinds of condensation agents. As for as we know, this is the first finding on "nucleation & growth" from a single polymer, not only DNA but also all the other synthetic and natural polymers. In addition to this, the relationship between gene-transfection efficency and higher-order structure of the DNA carrier-complex was discussed.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (66 results)

All Other

All Publications (66 results)

  • [Publications] Y.Yoshikawa: "Folding and Aggregation of DNA Chains Induced by Complexation with Lipospermine : Formation of a Nuclesome-Like Structure and Network Assembly" FEBS Lett.396. 71-76 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshikawa: "Daunomycin Unfolds Compactly Packed DNA" Biophys.Chem.61. 93-100 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.G.Starodoubtsev: "Intrachain Setregation in Single Giant DNA Molecules Induced by Poly (2-vinylpyrrolidone)" J.Phys.Chem.100. 19702-19705 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] V.V.Vasilevskaya: "Structure of Collapsed Persistent Macromolecule : Toroids vs. Spherical Globule" Biopolymers. 41. 51-60 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "Folding of Long DNA Chains in the presence of distearyldimethylammonium Bromide and Unfolding Induced by Neutral Liposomes" J.Chem.Soc.93. 283-288 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "First-Order Phase Transition in Large Single Duplex DNA Induced by a Nonionic Surfactant" Biochem.Biophys.Res.Commun.230. 514-517 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Emi: "Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate" Biochem.Biophys.Res.Commun.231. 421-424 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Y.Kramarenko: "Collapse of Polyelectrolyte Macromolecules Revisited" Macromolecules. 30. 3383-3388 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Complexity in a Molecular String : Hierchical Structure as Is Exemplified in aDNA Chain" Complexity and Diversity,Springer. 81-90 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "Cooperativity or phase transition? Unfolding transition of DNA cationic surfactant complex" J.Chem.Phys.107. 6917-6924 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Highly Effective Compaction of Long Duplex DNA Polyethylene Glycol with Pendant Amino Groups" J.Am.Chem.Soc.119. 6473-6477 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Noguchi: "First-order phase transition ina stiff polymer chain" Chem.Phys.Lett.230. 184-188 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Takahashi: "Discrete Coil-Globule Transition of Single duplex DNAs Induced by Polyaminese" J.Phys.Chem.B. 101. 9396-9401 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yamasaki: "Higher Order Structure of DNA Controlled by the Redox State of Fe2+/Fe3+" J.Am.Chem.Soc.119. 10573-10578 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ueda: "Stretching of Long DNA under Altemating Current Electric Fields in a Concentrated Polymer Solution" Polym.J.29. 1040-1043 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Folding transition in single long duplex DNA cahin" Progr.Colloid.Polym.Sci.106. 204-208 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "Cooperation between salt induced globule-coil transition in single duplex DNA complexed with cationic surfactant and sphere-rod transition of surfactant micelles" Progr.Colloid.Polym.Sci.106. 209-214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Ichiba: "Single Chain Observation on Collapse Transition in Giant DNA Induced by Negativery-Charged Polymer" Biochem.Biophys.Res.Commum.242. 441-445 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Marked Discreteness on the coil-globule transition of single duplex-DNA" Ber.Bunsenges.Phys.Chem.Vol.100. 876-880 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Nucleation and Growth in Single DNA Molecules" J.Am.Chem.Soc.Vol.118. 929-930 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Noguchi: "Self Organized Nanostructure Constructed with a Single Polymer Chain" Chem.Phys.Lett.Vol.261. 527-533 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ueda: "Phase Discrete Transition and Phase Segregation in a Single Double-Stranded DNA Molecule" Phys.Rev.Lett.Vol.77. 2133-2136 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Kidoaki: "The Folded State of Long Duplex-DNA Chain Reflects Its Solution History" Biophys.J.Vol.71. 932-939 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshikawa: "Folding and Aggregation of DNA Chains Induced by Complexation with Lipospermine : Formation of a Nuclesome-Like Structure and Network Assembly" FEBS Lett.Vol.396. 71-76 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshikawa: "Daunomycin Unfolds Compactly Packed DNA" Biophys.Chem.Vol.61. 93-100 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.G.Starodoubtsev: "Intrachain Setregation in Single Giant DNA Molecules Induced by Poly (2-vinylpyrrolidone)" J.Phys.Chem.Vol.100. 19702-19705 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] V.V.Vasilevskaya: "Structure of Collapsed Persistent Macromolecule : Toroids vs.Spherical Globule" Biopolymers. Vol.41. 51-60 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "Folding of Long DNA Chains in the presence of disteary Idimethylammonium Bromide and Unfolding Induced by Neutral Liposomes" J.Chem.Soc., Faraday Trans.Vol.93. 283-288 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "First-Order Phase Transition in Large Single Duplex DNA Induced by a Nonionic Surfactant" Biochem.Biophys.Res.Commun.Vol.230. 514-517 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Emi: "Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate" Biochem.Biophys.Res.Commum.Vol.231. 421-424 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Y.Kramarenko: "Collapse of Polyelectrolyte Macromolecules Revisited" Macromolecules. Vol.30. 3383-3388 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Complexity in a Molecular String : Hierchical Structure as Is Exemplified in a DNA Chain" Complexity and Diversity, Springer. 81-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "Cooperativity or phase transition? Unfolding transition of DNA cationic surfactant complex" J.Chem.Phys.Vol.107. 6917-6924 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Highly Effective Compaction of Long Duplex DNA Polyethylene Glycol with Pendant Amino Groups" J.Am.Chem.Soc.Vol.119. 6473-6477 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Noguchi: "First-order phase transition in a stiff polymer chain" Chem.Phys.Lett.Vol.278. 184-188 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Takahashi: "Discrete Coil-Globule Transition of Single duplex DNAs Induced by Polyamines" J.Phys.Chem.B. Vol.101. 9396-9401 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yamasaki: "Higher Order Structure of DNA Controlled by the Redox State of Fe2+/Fe3+" J.Am.Chem.Soc.Vol.119. 10573-10578 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ueda: "Stretching of Long DNA under Alternating Current Electric Fields in a Concentrated Polymer Solution" Polym.J.Vol.29. 1040-1043 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshikawa: "Folding transition in single long duplex DNA cahin" Progr.Colloid.Polym.Sci.Vol.106. 204-208 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.M.Mel'nikov: "Cooperation between salt induced globule-coil transition in single duplex DNA complexed with cationic surfactant and sphere-rod transition of surfactant micelles" Progr.Colloid.Polym.Sci.Vol.106. 209-214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.G.Starodoubtsev: "Conformational Transition of Large Duplex T4 DNA Embedded in Poly (acrylamide) Gel" Langmuir. Vol.14. 214-217 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Ichiba: "Single Chain Observation on Collapse Transition in Giant DNA Induced by Negativery-Charged Polymer" Biochem.Biophys.Res.Commun. Vol.242. 441-445 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshikawa: "Folding and Aggregation of DNA Chains Induced by Complexation with Lipospermine:Formation of a Nuclesome-Like Structure and Network Assembly" FEBS Lett.396. 71-76 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Yoshikawa: "Daunomycin Unfolds Compctly Packed DNA" Biophys.Chem.61. 93-100 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.G.Starodoubtsev: "Intrachain Setregation in Single Giant DNA Molecules Induced by Poly(2-vinylpyrrolidone)" J.Phys.Chem.100. 19702-19705 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] V.V.Vasilevskaya: "Structure of Collapsed Persistent Macromolecule:Toroids vs.Spherical Globule" Biopolymers. 41. 51-60 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.M.Mel‘nikov: "Folding of Long DNA Chains in the presence of distearyldimethylammonium Bromide and Unfolding Induced by Neutral Liposomes" J.Chem.Soc.93. 283-288 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.M.Mel‘nikov: "First-Order Phase Transition in Large Single Duplex DNA Induced by a Nonionic Surfactant" Biochem.Biophys.Res.Commun.230. 514-517 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Emi: "Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate" Biochem.Biophys.Res.Commum.231. 421-424 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Y.Kramarenko: "Collapse of Polyelectrolyte Macromolecules Revisited" Macromolecules. 30. 3383-3388 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Yoshikawa: "Complexity in a Molecular String:Hierchical Structure as Is Exemplified in a DNA Chain" Complexity and Diversity,Springer. 81-90 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.M.Mel‘nikov: "Cooperativity or phase transition?Unfolding transition of DNA cationic surfactant complex" J.Chem.Phys.107. 6917-6924 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Yoshikawa: "Highly Effective Compaction of LongDuplex DNA Polyethylene Glycol with Pendant Amino Groups" J.Am.Chem.Soc.119. 6473-6477 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Noguchi: "First-order phase transition in a stiff polymer chain" Chem.Phys.Lett.230. 184-188 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Takahashi: "Discrete Coil-Globule transition of Single duplex DNAs Induced by Polyaminase" J.Phys.Chem.B. 101. 9396-9401 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Yamasaki: "Higher Order Structure of DNA Controlled by the Redox State of Fe2+/Fe3+" J.Am.Chem.Soc.119. 10573-10578 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Ueda: "Stretching of Long DNA under Alterrnating Current Electric Fields in a Concentrated Polymer Solution" Polym.J.29. 1040-1043 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Yoshikawa: "Folding transition in single long duplex DNA chain" Progr.Colloid.Polym.Sci.106. 204-208 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.M.Mel‘nikov: "Cooperation between salt induced globule-coil transition in single duplex DNA complexed with cationic surfactant and sphere-rod transition of surfactant micelles" Progr.Colloid.Polym.Sci.106. 209-214 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Ichiba: "Single Chain Observation on Collapse Transition in Giant DNA Induced by Negativery-Charged Polymer" Biochem.Biophys.Res.Commum.242. 441-445 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉川研一他: "Marked Discreteness on the coil-globule transition of single duplex-DNA" Ber.Bunsenges.Phys.Chem.Vol.100. 876-880 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川研一他: "Nucleation and Growth in Single DNA Molecules" J.Am.Chem.Soc.Vol.118. 929-930 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川研一他: "Self Organized Nanostructure Constructed with a Single Polymer Chain" Chem.Phys.Lett.Vol.261. 527-533 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川研一他: "Structure of Collapsed Persistent Macromolecule : Toroids vs.Spherical Globule" Biopolymers. Vol.41. 51-60 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川研一他: "Phase Discrete Transition and phase Segregation in a Single Double-Stranded DNA Molecule" Phys.Rev.Lett.Vol.77. 2133-2136 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川研一他: "The Folded State of Long Duplex-DNA Chain Reflects Its Solution History" Biophys.J.Vol.71. 932-939 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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