| Project/Area Number | 08555137 |
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MATSUI Saburo Kyoto univ., Dept.of Engineering, Prof., 工学研究科, 教授 (90092808)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Tomonari Kyoto univ., Dept.of Engineering, Assistant Prof., 工学研究科, 助手 (50273488)
YAMADA Harumi Kyoto univ., Dept.of Engineering, Assistant Prof., 工学研究科, 助手 (40089123)
SHIMIZU Yoshihisa Kyoto univ., Dept.of Engineering, Associate Prof., 工学研究科, 助教授 (20226260)
MORISAWA Shinsuke Kyoto univ., Dept.of Engineering, Prof., 工学研究科, 教授 (50026340)
津野 洋 京都大学, 工学部, 教授 (40026315)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed(Fiscal Year 1998)
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| Budget Amount *help |
¥18,100,000 (Direct Cost : ¥18,100,000)
Fiscal Year 1998 : ¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1997 : ¥2,300,000 (Direct Cost : ¥2,300,000)
Fiscal Year 1996 : ¥13,600,000 (Direct Cost : ¥13,600,000)
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| Keywords | DNA adduct / mutation spectrum / biomarker / carcinogenic risk / nitrobenzanthrone / acetaldehyde / formaldehyde / 尿 / LC / MS / N2-ethyl-dG / DNA付加体 / MC / リスク評価 |
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| Research Abstract |
In developed nations, about 25% of people are killed by cancer. One reason is that other causes of death are decreasing, but it raise a question that such a high frequency of cancer death is inevitable or not. The types and the frequency of cancer are various among the world or region. This suggests that the environmental factors are important for development of cancers. Although various carcinogens have been identified from air, water environment and foods, it is difficult to monitor all the carcinogens by analytical approach. Bioassay is useful to evaluate the whole toxicity of environmental samples, but the biomonitoring is indispensable for evaluation of the risk. In this study, we focused on DNA damages for evaluation of cancer risk, and done the study about its structure identification and quantification. We identified the chemical structure of 3-nitrobenzanthrone derived DNA adducts. We also developed the method for quantification of DNA adducts in human urine samples which are induced by acetaldehyde, formaldehyde and oxidative stress. We also analyzed the mutation spectrum of those mutagens by using shuttle vector.
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