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Study on Submerged Culture of Mushroom Mycelium by Bubble Column Fermentors

Research Project

Project/Area Number 08555208
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionNARA NATIONAL COLLEGE OF TECHNOLOGY

Principal Investigator

KAWAGOE Mikio  Nara National College of Technology, Department of Chemical Engineering, Professor, 化学工学科, 教授 (90043489)

Co-Investigator(Kenkyū-buntansha) NAOE Kazumitsu  Nara National College of Technology, Department of Chemical Engineering, Lecture, 物質化学工学科, 講師 (00259912)
MIKI Koujirou  Nara National College of Technology, Department of Chemical Engineering, Associa, 物質化学工学科, 助教授 (80259910)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsBiochemical Engineering / Bubble Column / Bioreactor / Mushroom / Submerged Culture / Reverse Micelles / Extraction / Separation / 逆ミセル抽出 / 膜分離 / バイオアクター / バイオセンサー
Research Abstract

This study is composed of two parts ; the study on submerged culture of mushroom mycelium by bubble column bioreactors, and the study on separation of protein by reverse micelles.
I.Submerged culture of mushroom mycelium by bubble column bioreactors : We devised a novel type of airlift column with a tapered riser which is anticipated to be suitable to the submerged culture of mushroom mycelium. The hydrodynamics (gas holdup and liquid circulation velocity) and the oxygen transfer rate in the airlift column were investigated empirically and theoretically. Conditions to suspend large spherical pellets, which are often formed for the submerged culture of mushroom mycelium, in the standard bubble column were investigated. Next, submerged culture of mushroom mycelium was carried out using two species of mushroom : one is productipn of L-malic acid by Schizophyllum commune, and the other is large-scale culture of Tricholoma matsutake. The optimum conditions of the submerged culture on medium composition, temperature, and air flow rate were found for each mushroom.
2. Extraction of protein by reverse micelles : Since mushroom mycelium produces many useful proteins, extraction of protein by reverse micelles was investigated. We found the minimal and optimal AOT concentration for the extraction of lysozyme using reverse micelles. The minimal AOT concentration of a binary protein mixture containing cytocbrome c and lysozyme was found to be smaller than the sum of the minimal AOT concentration of each protein obtained in single protein systems. The addition of low concentration of guanidium salts into the feed solution as a pretreatment decreased the minimal AOT concentration, increased the forward extraction, and enabled the successful recoverry of the protein from the micellar organic phase, while the trehalose addition reduced the forward extraction. Using non-ionic Span 60 reverse micelles, cytochrome c was successfully extracted at concentration higher than the CMC.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (100 results)

All Other

All Publications (100 results)

  • [Publications] 河越幹男・前田孝司: "テーパ付エアーリフト気泡塔の流動特性" 化学工学論文集. 22巻・3号. 629-634 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe,Y.Nakamura and H.Noda: "Cultivation of Matsutake Mycelium in Bubble Column Bioreactors" 化学工学シンポジウムシリーズ“Horizon of Biochemical Engineering". 57号. 109-112 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越 幹男・三木 功次郎・直江 一光: "バイオ生産プロセスにおける培養・センシング・分離精製" '96国際食品工業展アカデミックプラザ. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越幹男・吉田尚平: "テーパ付エアーリフト気泡塔内の液流速分布及び液側混合特性" 化学工学会第29回秋季大会要旨集. H104 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe: "Application of the Bubble Column in Biotechnology" The Fourth Japan-Korea Symposium on Separation Technology. 623-626 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe: "Flow Characteristics and Mixing Properties in an External-Loop Airlift Column with a Tapered Riser" The Fourth Japan-Korea Symposium on Separation Technology. 231-234 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 三木功次郎・村井久子・木下英明・池田篤治: "電子伝達メディエータ存在下における真菌類固定化電極の電流応答" 96'電気化学秋季大会. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 村田昌代・直江一光・河越幹男・今井正直: "逆ミセルを用いたタンパク質抽出における塩酸グアニジン添加効果に対する温度効果" 化学工学会第61年会要旨集. H309 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe,S.Matsumura, Y.Mawatari,M.Kawagoe and M.Imai: "Selective Extraction of Subtilisin BPN'from Bacillus Subtilis Using Reverse Micelles" The Fourth Japan-Korea Symposium on Separation Technology. 409-412 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe,K.Yamamoto, M.Kawagoe and M.Imai: "Hydrodynamic Properties of Protein with low concentration of guanidine hydrochloride on solubilization into organized-assemblies" 3rd Workshop“Principles of Protein Architecture". 188 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 直江一光: "逆ミセルによるタンパク質分離の高効率化のための抽出条件" 第33回液膜研究会研究講演会. 21-29 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 喜多豊・直江一光・河越幹男・今井正直: "AOT逆ミセルを用いたタンパク質抽出における糖類添加の効果" 化学工学会第62年会. R111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe,K.Hyakumura,S.Suye,K.,Miki and K.Naoe: "Application of Bubble Column Fermenors to Submerged Culture of Schizophyllum commune for Production of L-Malic Acid" Journal of Fermentation and Bioengineering. Vol.84. 333-336 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越幹男・吉田尚平・石井幸昌: "テーパー付きエアーリフト気泡塔の流動及び混合特性" 化学工学会第30回秋季大会要旨集. G104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越幹男・三木功次郎・直江一光: "バイオ生産プロセスにおける培養・センシング・分離精製" '97国際食品工業展アカデミックプラザ. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe,S.Yoshida and Y.Ishii: "Hydrodynamics and Axial Liquid-Phase Mixing in an External-Loop Airlift Column with a Tapered Riser" Preprints of 4th Japanese/German Symposium“Bubble Columns". 226-231 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越幹男・上田光洋: "テーパ付きエアーリフト気泡塔の酸素移動特性に及ぼす温度の影響" 奈良工業高等専門学校研究紀要. 第33号. 115-118 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe,M.Tamai.,M.. Kawagoe,M.Imai,M.Shimizu: "Minimal AOT Concentration of binary protein component in reverse micellar Extration" Trans.IChemE,Part C. Vol.75. 143-147 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 直江一光: "逆ミセルを用いたタンパク質抽出における高機能化条件の探索" 抽出技術集覧(化学工学会関東支部、分離技術研究会編). 85-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Imai,T.Natsume,K.Naoe,M.Shimizu,S.Ichikawa and S.Furusaki: "Hydrophilic surroundings requisite for the solubilizatio of proteins related with their hydrophobicity in the AOT reversed micellar extraction" Bioseparation. Vol.6. 325-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] O.Ura,K.Naoe,M.Kawagoe and M.Imai: "Extraction conditions for protein in Span 60 reverse micellar system" “Biochemical Engineering:Marching toward the Century of Biotechnology"(Ed. by Z.Y.Shen,F.Ouyang,J.T.Yu,Z.A.Gao),Tsinghua Univ.Press (Beijing). 942-945 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe,M.Murata,M.Kawagoe and M.Imai: "Efficacy of guanidium salts in backward extraction of protein from reverse micellar org anic phase" “Biochemical Engineerng:Marching toward the Century of Biotechnology"(Ed. by Z.Y.Shen,F.Ouyang,J.T.Yu,Z.A.Cao),Tsinghua Univ.Press(Beijing). 926-929 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 浦 理、直江一光、河越幹男 今井正直: "Span60逆ミセルを用いたタンパク質の抽出条件" “液幕及び分子認識材料利用技術の基礎と応用",(化学工学会「液幕及び分子認識液体利用プロセス」特別研究会編). 147-154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe,O.Ura,M.Hattori,M.Kawagoe and M.Imai: "Protein Extraction in reverse micelles of Span 60" In“Advances in Bioseparation Engineerin 1997",(Ed.by The Special Study Group for Bioseparation Engineering of SCEJ),(1998). 91-96 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe,M.Yamamoto,Y.Kita,M.Kawgoe and M.Imai: "Comparison of the Effect of Guandium Salts and Trehalose in Reverse Micellar Protein Extraction" In“Advances in Bioseparation Engineering 1997",(Ed.by The Special Study Group for Bioseparation Engineering of SCEJ). 97-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe,K.Kawakami,Y.Nakamura,K.Naoe,K.Miki and H.Noda: "Submerged Culture of Tricholoma Matsutake Mycelium in Bubble Column Fermenors" Journal of Bioscience and Bioengineering. Vol.87. 116-118 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越幹男・田中智也・井口高行: "低密度大粒子懸濁気泡塔の流動特性" 化学工学会富山大会要旨集. G104 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 河越幹男・田中智也・林賢一・井口高行: "低密度大粒子懸濁気泡塔の流動特性" 奈良工業高等専門学校研究紀要. 第34号. 117-122 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naoe K.,Ura O.,Hattori M.,Kawagoe M.and Imai M.: "Protein extraction using non-ionic reverse micelles of Span 60" Biochem.Eng.J.22(2). 113-119 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naoe,K.,Kai,T.,Kawagoe,M.,and Imai,M.: "Extraction of flexibly structured protein in AOT reverse micelles: the flexible structure ofprotein is the dominant factor for its incorporation into reverse micelles" Biochem.Eng.J.3. 79-85 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naoe K.,Nishino M.,Ohsa T.,Kawagoe M.and Imai,M.: "Protein extraction using sugar ester reverse micelles" J.Chem.Technol.Biotechnol.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本真史,直江一光,河越幹男,今井正直: "AOT逆ミセル溶媒へのタンパク質の抽出挙動とタンパク質の水和" 化学工学会富山大会要旨集. A-15 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田中公廣,直江一光 河越幹男、今井正直: "両親媒性分子による水性二相分配系の形成とタンパク質分離" 化学工学会富山大会要旨集. A-16 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 浦 理,直江一光,河越幹男,今井正直: "Span 60逆ミセルにおけるタンパク質の可溶化状態" 化学工学会第31回秋季大会要旨集. H120 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Naoe,K.,T.Kai,M.Kawagoe,M.Imai: "Solubilization behavior of flexibly structured protein into amphiphile-assemblies" 4th Workshop“Principles of Protein Architecture". 234 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田中公廣,直江一光,河越幹男,今井正直: "Triton X-114による水性二相系を用いたタンパク質の分配挙動" 化学工学会第64年会要旨集. R305 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 太佐友美,直江一光,河越幹男,今井正直: "シュガーエステル逆ミセルによるタンパク質の抽出条件" 化学工学会第64年会要旨集. R314 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, and T.Maeoka: ""Flow Characteristics of an External-Loop Airlift Column with a Tapered Riser"" Kagaku kogaku Ronbunshu. 22. 629-634 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe: ""Application of the Bubble Column in Biotechnology"" The Fourth Japan-Korea Symposium on Separation Technology. 623-626 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe: "Flow Characteristics and Mixing Properties in an External-Loop Airlift Column with a Tapered Riser"" The Fourth Japan-Korea Symposium on Separation Technology. 231-234 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, Y.Nakamura, and H.Noda: ""Cultivation of Matsutake Mycelium in Bubble Column Bioreactors"" Chemical Engineering Symposium Series 57 <Horizon of Biochemical Engineering>. 109-112 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, K.Hyakumura, S.Suye, K.Miki, and K.Naoe: ""Application of Bubble Column Fermentors to Submerged Culture of Schizophyllum commune for Production of L-malic Acid"" J.Ferment.Bioeng. 84. 333-336 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, S.Yoshida and Y.Ishii: ""Hydrodynamics and Axial Liquid-Phase Mixing in an External-Loop Airlift Column with a Tapered Riser"" Preprints of 4th Japanese/German Symposium "Bubble Columns". 226-231 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, and M.Ueda: ""Effects of Temperature on Oxygen Transfer Rates in an Airlift Bubble Column with a Tapered Riser"" Research Reports of Nara National College of Technology. 33. 115-118 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, T.Tanaka, K.Hayashi, and T.Iguchi: ""Hydrodynamics of Three Phase Bubble Columns Containing Large Particles with Low Density"" Research Reports of Nara National College of Technology. 34. 117-122 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe, K.Kawakami, Y.Nakamura, K.Naoe, K.Miki, and H.Noda: ""Submerged Culture of Tricholoma matsutake Mycelium in Bubble Column Fermentors"" J.Biosci.Bioeng. 87. 116-118 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, M.Imai, and M.Shimizu: ""Optimal amphiphile concentration for lysozyme extraction using reverse micelles"" Trans.IchemE Part C. 74. 163-170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, M.Kawagoe, S.Hakoda, M.Imai, and M.Shimizu: ""Minimal AOT Concentration with Guanidine Hydrochloride in Reverse Micellar Protein Extraction"" The 1996 International Congress on Membrane and Membrane Processes. 838-839 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Murata, K.Naoe, M.Kawagoe, and M.Imai: ""Effect of Guanidine Hydrochloride on Protein Recovery from Reverse Micellar Organic Media"" The 1996 International Congress on Membrane and Membrane Processes. 840-841 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, S.Matsumura, Y.Mawatari, M.Kawagoe, and M.Imai: ""Selective Extraction of Subtilisin BPN from Bacillus Subtilis Using Reverse Micelles"" The Fourth Japan-Korea Symposium on Separation Technology. 409-412 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, Y.Yamamoto, M.Kawagoe, and M.Imai: ""Hydrodynamic Properties of Protein with Low Concentration of Guanidine Hydrochloride on Solubilization into Organized-assemblies"" 3rd Workshop "Priciples of Protein Architecture". 188 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, M.Tamai, M.Kawagoe, M.Imai, and M.Shimizu: ""Minimal AOT Concentration of binary protein component in reverse micellar EXtraction"" Trans.IChemE,Part C. 75. 143-147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Imai, T.Natsume, K.Naoe, M.Shimizu, S.Ichikawa and S.Furusaki: ""Hydrophilic surroundings requisite for the solubilization of proteins related with their hydrophobicity in the AOT reversed micellar extraction"" Bioseparation. 6. 325-333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] O.Ura, K.Naoe, M.Kawagoe and M.Imai: ""Extraction conditions for protein in Span 60 reverse micellar system"" In "Biochemical Engineering : Marching toward the Century of Biotechnology" (Ed.by Z.Y.Shen, F.Ouyang, J.T.Yu, Z.A.Cao), Tsinghua Univ.Press (Beijing). Vol.2. 942-945 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, M.Murata, M.Kawagoe, and M.Imai: ""Efficacy of guanidium salts in backward extraction of protein from reverse micellar organic phase"" In "Biochemical Engineering : Marching toward the Century of Biotechnology" (Ed.by Z.Y.Shen, F.Ouyang, J.T.Yu, Z.A.Cao), Tsinghua Univ.Press (Beijing). Vol.2. 926-929 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, Y.Kita, M.Yamamoto, Y.Kita, M.Kawagoe, and M.Imai: ""Stabilization effect of trehalose addition on protein solubilization into reverse micelles"" 4th Workshop "Principles of Protein Architecture". 264 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, Y.Kita, M.Yamamoto, M.Kawagoe, and M.Imai: ""Eifficacy of Trehalose on Protein Extraction Using Reverse Micelles"" Indonesian Biotechnology Conference, (Jakarta, Indonesia). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, O.Uea, M.Hattori, M.Kawagoe, and M.Imai: ""Protein Extraction in Reverse Micelles of Span 60"" In"Advances in Bioseparation Engineering 1997", (Ed.by The Special study Group for Bioseparation Engineering of SCEJ). 91-96. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, M.Yamamoto, Y.Kita, M.Kawagoe, and M.Imai: ""Comparison of the Effect of Guanidium Salts and Trehalose in Reverse Micellar Protein Extraction"" In "Advances in Bioseparation Engineering 1997", (Ed.by The Special Study Group for Bioseparation Engineering of SCEJ). 97-102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, O.Ura, M.Hattori, M.Kawagoe and M Imai: ""Protein extraction using non-ionic reverse micelles of Span 60"" Biochem.Eng.J.2. 113-119 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, T.Kai, M.Kawagoe, and M.Imai: ""Solubilization behavior of flexibly structured protein into amphiphile-assemblies"" 4th Workshop "Principles of Protein Architecture". 234 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, T.Kai, M.Kawagoe, and M.Imai: ""Extraction of flexibly structured protein in AOT reverse micelles : the flexible structure of protein is the dominant factor for its incorporation into reverse micelles"" Biochem.Eng.J.3. 79-85 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Naoe, M.Nishino, T.Ohsa, M.Kawagoe, and M.Imai: ""Protein extraction using sugar ester reverse micelles"" J.Chem.Technol.Biotechnol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Kawagoe,K.Kawakami,Y.Nakamura,K.Naoe,K.Miki and H.Noda: "Submerged Culture of Tricholoma Matsutake Mycelium in Bubble Column Fermenors" Journal of Bioscience and Bioengineering. Vol.87. 116〜118 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 河越幹男・田中智也・井口高行: "低密度大粒子懸濁気泡塔の流動特性" 化学工学会富山大会要旨集. G104 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 河越幹男・田中智也・林賢一・井口高行: "低密度大粒子懸濁気泡塔の流動特性" 奈良工業高等専門学校研究紀要. 第34号. 117-122 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naoe K.,Ura O.,Hattori M.,Kawagoe M.and Imai M.: "Protein extraction using nonionic reverse micelles of Span 60" Biochem.Eng.J.2(2). 113-119 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naoe,K.,Kai,T.,Kawagoe,M.,and Imai,M.: "Extraction of flexibly structured protein in AOT reverse micells: the flexible structure of protein is the dominant factor for itsincorporation into reverse micelles" Biochem.Eng.J.3. 79-85 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naoe K.,Nishino M.,Ohsa T.,Kawagoe,M.and Imai,M.: "Protein extraction using sugar ester reverse micelles" J.Chem.Technol.Biotechnol.in press (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山本真史,直江一光,河越幹男,今井正直: "AOT逆ミセル溶媒へのタンパク質の抽出挙動とタンパク質の水和" 化学工学会富山大会要旨集. A-15 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田中公廣,直江一光,河越幹男,今井正直: "両親媒性分子による水性二相分配系の形成とタンパク質分離" 化学工学会富山大会要旨集. A-16 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 浦 理,直江一光,河越幹男,今井正直: "Span 60逆ミセルにおけるタンパク質の可溶化状態" 化学工学会第31回秋季大会要旨集. H120 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Naoe,K.,T.Kai,M.Kawagoe,M.Imai: "Solubilization behavior of flexibly structured protein into amphiphile-assemblies" 4th Workshop "Principles of Protein Architecture". 234 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田中公廣,直江一光,河越幹男,今井正直: "Triton X-114による水性二相系を用いたタンパク質の分配挙動" 化学工学会大64年会要旨集. R305 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 太佐友美,直江一光,河越幹男,今井正直: "シュガーエステル逆ミセルによるタンパク質の抽出条件" 化学工学会第64年会要旨集. R314 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Kawagoe,K.Hyakumura,S.Suye,K.Miki and K.Naoe: "Application of Bubble Column Fermenors to Submerged Culture of Schizophyllum commune for Production of L-Malic Acid" Journal of Fermentation and Bioengineering. Vol.84. 333〜336 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 河越幹男・吉田尚平・石井幸昌: "テ-パ-付きエア-リフト気泡塔の流動及び混合特性" 化学工学会第30回秋季大会要旨集. G104 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 河越幹男・三木功次郎・直江一光: "バイオ生産プロセスにおける培養・センシング・分離精製" '97国際食品工業展アカデミックプラザ. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Kawagoe,S.Yoshida and Y.Ishii: "Hydrodynamics and Axial Liquid-Phase Mixing in an External-Loop Airlift Column with a Tapered Riser" Preprints of 4th Japanese/German Symposium"Bubble Columns". 226-231 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 河越幹男・上田光洋: "テ-パ付きエア-リフト気泡塔の酸素移動特性に及ぼす温度の影響" 奈良工業高等専門学校研究紀要. 第33号. 115-118 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Naoe,M.Tamai.,M.Kawagoe M.Imai,M.Shimizu: "Minimal AOT Concentration of binary protein component in reverse micellar Extraction" Trans.IChemE,Part C. Vol.75. 143-147 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 直江一光: "逆ミセルを用いたタンパク質抽出における高機能化条件の探索" 抽出技術便覧(化学工学会関東支部、分離技術研究会編). 85-92 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Imai,T.Natsume,K.Naoe,M.Shimizu,S.Ichikawa and S.Furusaki: "Hydrophilic surroundings requisite for the solubilization of Proteins related with their hydrophobicity in the AOT reversed micellar extraction" Bioseparation. Vol.6. 325-333 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] O.Ura,K.Naoe,M.Kawagoe and M.Imai: "Extraction conditions for protein in Span 60 reverse micellar system" "Biochemical Engineering:Marching toward the Century of Biotechnology"(Ed.by Z.Y.Shen,F.Ouyang,J.T.Yu,Z.A.Cao),Tsinghua Univ.Press(Beijing). 942-945 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Naoe,M.Murata,M.Kawagoe and M.Imai: "Efficacy of guanidium salts in backward extraction of protein from reverse micellar organic phase" "Biochemical Engineering:Marching toward the Century of Biotechnology"(Ed.by Z.Y.Shen,F.Ouyang,J.T.Yu,Z.A.Cao),Tsinghua Univ.Press(Beijing). 926-929 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 浦 理、直江一光、河越幹男 今井正直: "Span 60逆ミセルを用いたタンパク質の抽出条件" "液幕及び分子認識材料利用技術の基礎と応用",(科学工学会「液幕及び分子認識液体利用プロセス」特別研究会編). 147-154 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Naoe,O.Ura,M.Hattori,M.Kawagoe and M.Imai: "Protein Extraction in reverse micelles of Span 60" "Advances in Bioseparation Engineering 1997"(Ed.by The Special Study Group for Bioseparation Engineering of SCEJ). 91-96 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Naoe,M.Yamamoto,Y.Kita,M.Kawagoe and M.Imai: "Comparison of the Effect of Guanidium Salts and Trehalose in Reverse Micellar Protein Extraction" "Advances in Bioseparation Engineering 1997"(Ed.by The Special Study Group for Bioseparation Engineering of SCEJ). 97-102 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 河越幹男・前岡孝司: "テ-パ付エア-リフト気泡塔の流動特性" 化学工学論文集. 22巻・3号. 629〜634 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kawagoe,Y.Nakamura and H.Noda: "Cultivation of Matsutake Mycelium in Bubble Column Bioreactors" 化学工学シンポジウムシリーズ″Horizon of Biochemical Engineering″. 57号. 109〜112 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 河越幹男・三木功次郎・直江一光: "バイオ生産プロセスにおける培養・センシング・分離精製" '96国際食品工業展アカデミックプラザ. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 河越幹男・吉田尚平: "テ-パ付エア-リフト気泡塔内の液流速分布及び液側混合特性" 化学工学会第29回秋季大会要旨集. H104 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kawagoe: "Application of the Bubble Column in Biotechnology" The Fourth Japan-Korea Symposium on Separation Technology. 623〜626 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kawagoe: "Flow Characteristics and Mixing Properties in an External-Loop Airlift Column with a Tapered Riser" The Fourth Japan-Korea Symposium on Separation Technology. 231〜234 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三木功次郎・村井久子・木下英明・池田篤治: "電子伝達メディエータ存在下における真菌類固定化電極の電流応答" 96'電気化学秋季大会. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村田昌代・直江一光・河越幹男・今井正直: "逆ミセルを用いたタンパク質抽出における塩酸グアニジン添加効果に対する温度効果" 化学工学会第61年会要旨集. H309 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Naoe,S.Matsumura,Y.Mawatari,M.Kawagoe and M.Imai: "Selective Extraction of Subtilisin BPN' from Bacillus Subtilis Using Reverse Micelles" The Fourth Japan-Korea Symposium on Separation Technology. 409〜412 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Naoe,K.Yamamoto,M.Kawagoe and M.Imai: "Hydrodynamic Properties of Protein with low concentration of guanidine hydrochloride on solubilization into organized-assemblies" 3th Workshop "Principles of Protein Architecture". 188 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 直江一光: "逆ミセルによるタンパク質分離の高効率化のための抽出条件" 第33回液膜研究会研究講演会. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 喜多豊・直江一光・河越幹男・今井正直: "AOT逆ミセルを用いたタンパク質抽出における糖類添加の効果" 化学工学会第62年会. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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