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DEVELOPMENT OF CELL HYBRID ARTIFICIAL BONE USING 3-D CELL CULTURE SYSTEM

Research Project

Project/Area Number 08557114
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Surgical dentistry
Research InstitutionTOKYO MEDICAL AND DENTAL UNIVERSITY

Principal Investigator

ASAHINA Izumi  TOKYO MEDICAL AND DENTAL UNIVERSITY,FACULTY OF DENTISTRY,ASSISTANTPROFESSOR, 歯学部, 講師 (30221039)

Co-Investigator(Kenkyū-buntansha) KASUGAI Shohei  TOKYO MEDICAL AND DENTAL UNIVERSITY,FACULTY OF DENTISTRY,ASSOSIATEPROFESSOR, 歯学部, 助教授 (70161049)
OIDA Shinitiro  TOKYO MEDICAL AND DENTAL UNIVERSITY,FACULTY OF DENTISTRY,INSTRUCTOR, 歯学部, 助手 (10114745)
HARADA Kiyoshi  TOKYO MEDICAL AND DENTAL UNIVERSITY,FACULTY OF DENTISTRY,INSTRUCTOR, 歯学部, 助手 (30228639)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsARTIFICIAL BONE / BMP / FGF / BONE MARROW CELL / HYDROXYAPATITE / ハイブリッド / bFGF / 再生 / 成長因子
Research Abstract

(1) Effects of Bone Morphogenetic Protein (BMP and basic Fibroblast Growth Factor (bFGF) on Bone Marrow Cel1s : We examined the effects of BMP and bFGF on rat bone marrow cells, and revealed that BMP stimulated both cell proliferation and differentiation, while bFGF stimulated cell proliferation but inhibited cell differentiation. bFGF, especially, enhanced the proliferation of osteoprogenitor cells, which had not diffeientiated into matures osteoblast yet. Therefor, the treatment of bFGF at the early stage of osteoblastic differentiation increased the. number of osteoprpgenitor cells and the treatment of BMP at the later stage induced differentiation of osteoblast. As a result the treatment of both BMP and bFGF at the optimal stage of osteoblastic differentiation promoted osteogenesis.
(2) Fabrication of Cell Hybrid Artificial Bone : New bone formation was induced in rat subcutaneous tissue by implanted bone marrow cells cultured in porous hydroxyapatite. The treatment of BMP and/or bEOF during the culture in vitro enhanced bone formation in vivo. We also examined porous poly (gycoic, lactic acid co-polymer) as a scaffold for 3-D cell culture, but cells did not infiltrate deeply into the block because of it7s hydrophobicity.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 東中川 真木子: "Basic FGF ならびにOP-1/BMP-7の骨髄細胞に対する作用とハイブリッド型人工骨の開発" 口腔病学会誌. 印刷中 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 朝比奈 泉: "骨再生の分子機構と骨形成蛋白質(BMP)を用いたほそ欠損修復への応用" 臨床科学. 34・9. 1261-1268 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I Asahina,et.al: "Repair of Bone Defect in Primate Mandible using a Bone Morphogenetic Protein (BMP)-Hydroxyapatite-Collagen Composite" J.Med.Dent.Sci.44・3. 63-70 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S Oida,et.al: "Biological Activities of Bone Extract containing TGF-β superfamily proteins" J.Hard Tiss.Biol.7・1. 27-36 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y Morotome et.al: "Gene Expression of Growth and Differentiation Factors -506-7 in Developing Bovine Tooth at the Root Forming stage" BBRC. 224・1. 85-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Laczka・Osyczk,etal.: "Behavior of bone marrow cells cultured on three different cocting of gel-derived bioactive glass-ceramics at early stage of cell differentiation" J Biomed Mater.Res.42・3. 433-442 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I Asahina, TK Sampath PV Hauschka: "Human Osteogenic Protein-1 Induces Chondroblastic, Osteoblastic, and/or Adipocytic Differentiation of Clonal Murine Target Cells" Experimental Cell Research. 222. 38-47 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamazaki Y,Oida S,Ishihara K,Nakabayashi, N.: "Ectopic induction of cartilage and bone by bovine bone morphogenetic protein using a biodegradable polymeric reservior." J Biomed.Mater.Res.30. 1-4 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Isobe M and Yamazaki Y Oida S,Ishihara K., Nakabayashi N,Amagasa, T.: "Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer." J.Biomed.Mater.Res.32. 433-438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Goseki M,Omi N,Yamamoto A,Oida S,Ezawa I,Sasaki S: "Ovariectomy decreases osteogenetic activity in rat bone." J.Nutr.Sci.Vitaminol.42. 55-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Iimura T,Baba O.Maruoka Y,Takeda K,Sasaki S,Shimokawa H,Oida S.: "Homeobox gene expression during bone formation induced by BMP." Annals New York Academy of Sciences. 785. 274-277 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I Asahina, M Watanabe, N Sakurai, M Mori, S Enomoto: "Repair of Bone Defect in Primate Mandible using a Bone Morphogenetic Protein (BMP) -Hydroxyapatite-Collagen Composite" J Med.Dent.Sci.44. 63-70 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oida, S.: "Bone regeneration by differentiation factors (BMPs) ." Tissue engineering for therapeuticuse 1 : Organ regeneration eddited by Ikade, Y.1998.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morotome Y,Goseki-Sone M,Ishikawa I,Oida S: "Gene expression of growth and differentiation factor-5, -6, -7 in developing bovine tooth at the root forming stage" Biochm.Biophys.Res.Comm.244. 85-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oida S,Yamazaki Y,Mori M,Asahina I: "Biological activities of bone extract containing TGF-b superfamily proteins" J.Hard Tiss.Biol.7. 27-34 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Duarte WR.Kasugai S.Iimura T.Oida S.Takenaga K.Ohya K.Ishikawa I.: "cDNA cloning of S100 calcium-binding proteins from bovine periodontal ligament and their expression in oral tissues." Journal of Dental Research. 77 (9). 1694-1699 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Laczka-Osyczka A.Laczka M.Kasugai S.Ohya K.: "Behavior of bone marrow cells cultured on three different coatings of gel-derived bioactive glass-ceramics at early stages of cell differentiation." Journal of Biomedical Materials Research.42 (3). 433-442 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Duarte WR.Iimura T.Oida S.Takenaga K.Ohya K.Ishikawa I.Kasugai S.: "Extracellular role of S100A4 calcium-binding protein in the periodontal ligament." Biochemical and Biophysical Research Communications.255 (2). 416-420 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda S.Kasugai S.Oida S.Iimura T.Ohya K.Ohyama T.Anabolic: "effect of amino-terminally truncated fibroblast growth factor 4 (FGF4) In bone." Bone.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M Higashinakagawa: "Biological Effects of Basic Fibroblast Growth Factor (bFGF) and Osteogenic Protein-1 (OP-1/BMP-7) on Bone Marrow Cells and Development of a Cell Hybrid Artificial Bone" J Stomatological Society.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 朝比奈 泉: "骨再生の分子機構と骨形成蛋白質(BMP)を用いた骨欠損修復への応用" 臨床科学. 34・9. 1261-1268 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Oida et al.: "Biological Activities of Bone Extract Containing TGF-β superfamily Proteins" Journal of Hard Tissue Biology. 7・1. 27-36 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Morotome.et al: "Gene Expression of Growth and Differetiation Factors-S,-6,and-7 in Developing Bovine Tooth at the Root Forming stage" Biochemical and Biophysical Research Communications.224・1. 85-90 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Abe.et al: "Inhibitory effects of 1.25-dihydroxyvitamin D3 and 9-Cis-retinorc acid on parathyroid hormone-related protein expression by oral cancer cell" Journal of Endcrinology. 156. 349-357 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Laczka-Osyczk et al: "Behavior of bone marrow cells cultured on three different coating of gel-derived bioactive glass-ceramics at early stage of cell differentation." Journal of Biomedical Materials Research. 42・3. 433-442 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Izumi Asahina: "Repair of Bone Defect in Primate mardilde using a Bone Morphogenetic Protein(BMP)-Hydroxyapatite-Collagen Composite" Journal of Medical and Pental Science. 44・3. 63-70 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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