|Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Molecular species of collagen in various tissues of tiger puffer, Takifugu rubripes, and yellow sea bream, Dentex tumifrons, were examined by biochemical tequniques. Two molcular species of collagen were isolated from the pepsin-solubilized collagens from muscle and skin of both fishes. By analyzes of SDS-polyacrylamide gel electrophoresis and amino acid composition, the major and mainor collagens from each tissue of both fishes were demonstrated to be Type I and V collagens, respectively. Other molecular speies of collagen were not detected in varous tissues of tiger puffer in the present study.
It was attempted to promote the primary culture of the cells derived from central nervous system (CNS) of tiger puffer. Concequently, the conditions for isolation of CNS cells, optimum composition, serum to be supplemented, and sdequate incubation temperature were established. Of the 21 sorts of cell adhesion molecules, including collagens from various species of fishes and marine invertebrates, Type V collagen from the skin of tiger puffer inhibited attachment and/or growth of CNS cells. On the different substrate, the cells has a tendency to show different morphological feature. Taking these results, it turned out that culture system of tiger puffer CNS cells would be utilized in several biological fields.