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Brain mechanism mediating fasting-induced suppression of reproductive function

Research Project

Project/Area Number 08660342
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNagoya University

Principal Investigator

TSUKAMURA Hiroko  Nagoya University, School of Agricultural Sciences, Assistant Professor, 農学部, 助手 (00212051)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsluteinizing hormone / fasting stress / estrogen / estrogen receptor / A2 region of solitary tract nucleus / paraventricular nucleus / noradrenergic neurons / 延髄孤束核A2領域 / ノルアドレナリン作働性神経
Research Abstract

Fasting-stress profoundly suppresses luteinizing hormone (LH) secretion in the rat in a estrogen dependent manner. It has been demonstrated that this suppression is mediated by noradrenergic neurons projecting to the hypothalamic paraventricular nucleus (PVN). The present research aimed to investigate further brain mechanism mediating the fasting-induced suppression of LH release.
Forty-eight h fasting significantly increased numbers of estrogen receptor-containing cells in both the PVN and A2 region of solitary tract nucleus (NTS) in the lower brain stem. Acute infusion of estrogen into the PVN caused an inhibition of pulsatile LH release only in the 48-h fasted ovariectomized (OVX) rats, but not in the ad lib-fed OVX rats. On the other hand, the estrogen infusion into the NTS did not affect LH secretion. The estrogen infusion into the both nuclei did not affect the noradrenaline release in the PVN.These results suggest that fasting induces estrogen receptor expression in the PVN where estrogen acts to suppresses LH secretion without affecting noradrenaline release.
It has demonstrated that intravenous injection of 2-deoxyglucose (2DG), a glucose antagonist, suppressed pulsatile LH release in OVX rats in a dose-dependent manner and that estrogen enhanced the inhibitory effect of 2DG on LH pulses. Furthermore, 2DG induced noradrenaline release in the PVN,and local injection of catecholamine synthesis inhibitor into the PVN blocked 2DG-induced suppression of LH secretion. These results suggest that suppression of LH release by lowered glucose availability is mediated y noradrenergic neurons projecting the PVN.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(35 results)

All Other

All Publications

  • [Publications] Nagatani, Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinology. 137. 1166-1170 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murahashi, Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinology. 137. 1171-1176 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats." Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Estacio, M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats." Brain Research. 717. 55-61 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 束村, 博子: "ストレスと生殖をめぐる神経内分泌機構" ヒューマンサイエンス. 8. 89-97 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats." Celluler and Molecular Neurobiology. 16. 311-324 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Evidence for terminal regulation of GnRH release by excitatory amino acids in the median eminence in female rats:A dual immunoelectronmicroscopical study." Endocrinology. (in press.). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, Kei-ichiro: "Neural Control of Reproduction.Physiology and Behavior" Japan Scientific Society Press/CRC Press, 254 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 束村 博子: "脳と生殖-GnRH神経系の進化と適応" 学会出版センター, 233 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats" Endocrinology. 137. 1166-1170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murahashi, Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem" Endocrinology. 137. 1171-1176 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats" Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Estacio, M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats" Brain Research. 717. 55-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "Paraventricular norepinephrine release mediates glucoprivic suppression of pulsatile LH secretion" Endocrinology. 137. 3138-3186 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats" Celluler and Molecular Neurobiology. 16. 311-324 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Glial and neuronal localization of neuronal nitric oxide synthase immunoreactivity in the median eminence of female rats" Brain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Evidence for terminal regulation of GnRH release by excitatory amino acids in the median eminence in female rats : A dual immunoelectronmicroscopical study" Endocrinology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murahashi, Kumiko: "Increase in in vitro glutamate release in the mediobasal hypothalamus during progesterone-enhanced LH surge in estrogen-primed ovariectomized rats" Journal of Reproduction and Development. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani,Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinology. 137. 1166-1170 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murahashi,Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinology. 137. 1171-1176 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] :Nagatani,Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats." Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Estacio,M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats." Brain Research. 717. 55-61 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 束村博子: "ストレスと生殖をめぐる神経内分泌機構" ヒューマンサイエンス. 8. 89-97 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda,Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats." Celluler and Molecular Neurobiology. 16. 311-324 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawakami,Shin-ichi: "Evidence for terminal regulation of GnRH release by excitatory amino acids in the median eminence in female rats:A dual immunoelectronmicroscopical study." Endocrinology. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda,Kei-ichiro: "Neural Control of Reproduction.Physiology and Behavior" Japan Scientific Society Press/CRC Press, 254 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 束村博子: "脳と生殖-GnRH神経系の進化と適応" 学会出版センター, 233 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagatani,Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinology. 137. 1166-1170 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Murahashi,Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinology. 137. 1171-1176 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagatani,Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats." Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Estacio,M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats." Brain Research. 717. 55-61 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 前多敬一郎: "生殖機能を制御する脳内メカニズム:GnRH Pulse Generator." 獣医畜産新報. 49. 33-38 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 束村博子: "ストレスと生殖をめぐる神経内分泌機構" ヒューマンサイエンス. 8. 89-97 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda,Kei-ichro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats." Celler and Molecular Neurobiology. 16. 311-324 (1966)

    • Related Report
      1996 Annual Research Report
  • [Publications] Estacio.M.A.C.: "Vagus nerve mediates the increase in estrogen receptors the hypothalamic paraventricular nucleus and nucleus of the slitary tract during fasting in ovariectomized rats." Neuroscience Letters. 208. 25-28 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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