Immunohistochemical study on the structure and function of the ductal system of digestive gland
Grant-in-Aid for Scientific Research (C)
General anatomy (including Histology/Embryology)
|Research Institution||Kitasato University|
YAMASHINA Shohei Kitasato Univ.Professof, 医学部, 教授 (90013987)
KADOYA Yuichi Kitasato Univ.Research Associate, 医学部, 助手 (10185887)
TAMAKI Hideaki Kitasato Univ.Assistant Professor, 医学部, 講師 (30155246)
SEGAWA Akihisa Kitasato Univ.Assistant Professor, 医学部, 講師 (50154638)
SAKAI Yasuhiro Kitasato Univ.Associate Professor, 医学部, 助教授 (00050625)
|Project Fiscal Year
1996 – 1997
Completed(Fiscal Year 1997)
|Budget Amount *help
¥1,900,000 (Direct Cost : ¥1,900,000)
Fiscal Year 1997 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1996 : ¥1,000,000 (Direct Cost : ¥1,000,000)
|Keywords||Digestive gland / Duct system / Regeneration / Development / Cell proliferation / Apoptos is / Immunohistochemistry / EGF / 消化腺 / 導管系 / 再生 / 発生 / 細胞増殖 / アポトーシス / 免疫組織化学 / 外分泌腺 / 導管 / 細胞分裂 / アポトシース / PCNA / TUNEL|
(1) Identification of Kalliklein containing cell in the salivary duct system
By immunoelectronmicroscopy, kalliklein was localized in small vesicles of striated duct cells, of which secretion was induced by alpha-adrenergic and cholinergic stimuli.
(2) Detection of proliferating activity in the exocrine duct cells
The BrdU method indicated that DNA synthetic activity was the highest in acinar and cetroacinar cells of the rat pancreas, and these cells were suggested to be potential proliferating cells.
(3) Cellular proliferation and apoptosis in the developing rat parotid gland
Proliferation and apoptosis was investigated in the developing parotid gland by immunohistochemical method. As a result, branching morphogenesis of parotid gland was suggested to proceed by proliferation of cells solely located in the terminal portion of immature duct system, and during the course no apoptosis was found to take place.
(4) Cell kinetics in regeneration after partial pancrectomy
Pancreas of rat was partia
lly removed and proliferating activity during the course of regeneration was studied. From 3 days after the operation, proliferation started in the acinar portion which continued until 2 weeks. Compensation of cells by regeneration was concluded to take place by the proliferation of terminal cells. This result was in good agreement with the one described in (2).
(5) Effect of EGF in the branching morphogenesis
Branching morphogenesis of embryonic mouse submandibular gland was accerelated by EGF,but EGF inhibitor was found to suppress the course. And EGF receptor was localized in the basal portion of the ductal cells at the particular stage of morphogenesis. From these observation, EGF was concluded to play an important role in the branching morphogenesis.
(6) Ultrastructure of salivary basement membrane
Basement membrane has been indicated to be the site of epithelial-mesenchymal interaction of morphogenesis, since its ultra-fine structure was investigated by high resolution electron microscopy. As-a result, salivary basement membrane was uniform and fine reticular struture of about 40-100nm thickness with vertical fibrils of 100nm diameter. Lamina rara was very likely to be an artificial structure due to fixation. Less
Research Output (12results)