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ANALYSIS OF IGA NETWORK BY GENE TARGETING

Research Project

Project/Area Number 08670149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TAKAI Toshiyuki  DEPARTMENT OF BIOTECHNOLOGY,FACULTY OF ENGINEERING,OKAYAMA UNIVERSITY,ASSOCIATE PROFESSOR, 工学部, 助教授 (20187917)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsGENE TARGETING / FC RECEPTOR / IMMUNOREGULATION / ALLERGY / GENE-DEFICIENT MICE / IGA / NK RECEPTOR / INHIBITORY SIGNAL / 遺伝子ターゲティング / 免疫制御
Research Abstract

We hae found a novel murine cell-surface glycoprotein, designated as q91, expressed mainly in myeloid cells such as macrophages and mast cells. The molecule has six immunoglobulin-line extracellular domains, a transmembrane segment, and a cytoplasmic tail containing four immunoreceptor tyrosine-based inhibition motif (ITIM) or ITIM-like sequences, resembling the structural features of human killer-cell inhibitory receptors (KIR). p91 comprises a polymorphic gene family, harboring one potent inhibitory-type p91 and at least two other p91 genes.
Tyrosine-phosphorylated, but not nonphosphorylated, synthetic peptides matching each of the third ITIM and the fourth ITIM-like sequences found in the cytoplasmic portion of p91A,the sole inhibitory-type p91, associated with the tyrosine phosphatases SHP-1 and SHP-2. In addition, the phosphotyrosyl peptide matching the third ITIM sequence also bound the inositol 5-phosphatase SHIP.
Thses results support the notion that p91A may function as an inhibitory cell-surface molecule against cell activation. The p91 genes were shown to be clustered in the proximal region of mouse chromosome 7, a syntenic position of human chromosome 19 where the genes for the KIR family are found. A human cDNA clone cross-hybridizing to a murine p91 probe was isolated from a human spleen cDNA library, and was found to be coding for a molecule quits similar to members of the immunoglobulin-like transcript (or ILT) family. The gene was found to locate on human chromosome 19q13.3-13.4. These results will establish the existence of a novel set of potent regulatory receptors in mouse and man, similar but different from the KIR family.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (81 results)

All Other

All Publications (81 results)

  • [Publications] 高井 俊行 ら: "FcγRIIノックアウトマウスにおけるマスト細胞の異常活性化" 臨床免疫. 29. 227-234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "Fcレセプターと抗体産生制御" 臨床免疫. 29. 898-904 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "ノックアウトマウスによる免疫研究" 実験医学. 15. 50-54 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "Fcレセプター欠損マウス" Molecular Medicine. 34. 44-54 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "FcγRノックアウトマウスを用いたアレルギー発症機能の解析" アレルギーの臨床. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行 ら: "FcレセプターによるB細胞の抑制" 臨床免疫. 29. 550-555 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "Fcレセプターによる免疫制御機構" 生化学. 69. 394-409 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "Fcレセプターの新展開" 感染・炎症・免疫. 27. 80-89 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuroiwa, A., et al.: "Association of tyrosine phosphatases SHP-1 and SHP-2,and inositol 5-phosphatase SHIP with gp49B1,and chromosomal assignment of the gene" J.Biol.Chem.273. 1070-1074 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamashita, Y., et al.: "Association of tyrosine-phosphorylated cytoplasmic motifs of p91A with tyrosine- and inositol-phosphatases." J.Biochem.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watanabe, N., et al.: "Antigen receptor cross-linking by anti-immunoglobulin antibodies coupled to cell surface membrane induces rapid apoptosis of normal spleen B cells." Scand.J.Immunol.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, A., et al.: "Requirement of tyrosine phosphatase SHP-1 and SHP-2 for PIR-B-mediated inhibitory signal." J.Exp.Med.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "Fcレセプターとアレルギー" 日本皮膚科学会雑誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行 ら: "Fcγ受容体" 現代医療. 30. 290-293 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "FcRノックアウトマウスを用いたアレルギー発症機構の解析" アレルギー科. 4. 471-477 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行: "FcRとKIRとの境目" JSI Newsletter. 5. 5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Takai, et al.: "Regulation of murine hypersensitive responses by Fc receptors." Allergology International. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行 ら: "アレルギーとFcレセプター" Molecular Medicine. 34. 1464-1476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hayami, K., et al.: "Molecular cloning of a novel murine cell-surface glycoprotein homologous to killer cell inhibitory receptors." J.Biol.Chem.272. 7320-7327 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hikida, M., et al.: "Characterization of B cells expressing recombination activating genes in germinal centers of immunized mouse lymph nodes." J.Immunol.158. 2509-2512 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Takai and J.V.Ravetch: "Eds J.G.J.van de Winkel and P.Mark Hogarth,Kluwer Academic Publishers," Immunoglobulin Receptors and their Physiological and Pathological Roles in Immunity. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行(分担執筆): "中外医学社" Annual Review 免疫1998. 312-320 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行(分担執筆): "中山書店" 「ノックアウトマウス・データブック」. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Takai(編集協力): "Knockout Mice." Edited by Tak W.Mak,Academic Press. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行(分担執筆): "「標準免疫学」" 医学書院, 390-401 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 俊行(分担執筆): "実験医学別冊「免疫研究の最前線′97-′98」" 羊土社, 172-179 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takai, T., et al.: "Regulation of murine hypersensitive resopnses by Fc receptors." Allergology Internaitonal. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hayami, K., et al.: "Molecular cloning of a novel murine cell-surface glycoprotein homologous to killer cell inhibitory receptors" J.Biol.Chem.272. 7320-7327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hikida, M., et al.: "Characterization of B cells expressing recombination activating genes in germinal centers of immunized mouse lymph nodes." J.Immunol.158. 2509-2512 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kuroiwa, A., et al.: "Association of tyrosine phosphatases SHP-1 and SHP-2, and inositol 5-phosphatase SHIP with gp49B1, and chromosomal assignment of the gene." J.Biol.Chem.273. 1070-1074 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamashita, Y., et al.: "Genomic structures and chromosomal location of p91, a novel murine regulatory receptor family." J.Biochem.123. 358-368 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watanabe, N., et al.: "Antigen receptor cross-linking by anti-immunoglobulin antibodies coupled to cell surface membrane induces rapid apoptosis of normal spleen B cells." Scand.J.Immunol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, A., et al.: "Requirement of tyrosine phosphatase SHP-1 and SHP-2 for PIR-B-mediated inhibitory signal" J.Exp.Med. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takai, T., et al.: Fe receptor genetics and the manipulation of genes in the study of FcR biology.In : Immunoglobulin Receptors and their Physiological and Pathological Roles in Immunity (Eds J.G.J.van de Winkel and P.Mark Hogarth), Kluwer Academic Publishers, Netherland, (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takai, T.: FcRgamma, Fcgamma RII Knockout mice.In : Knockout Mice. (Eds.Tak W.Maak), Academic Press, (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井俊行ら: "FcγRIIノックアウトマウスにおけるマスト細胞の異常活性化" 臨床免疫. 29. 227-234 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプターと抗体産生制御" 臨床免疫. 29. 898-904 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "ノックアウトマウスによる免疫研究" 実験医学. 15. 50-54 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプター欠損マウス" Molecular Medicine. 34. 44-54 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "FcγRノックアウトマウスを用いたアレルギー発症機構の解析" アレルギーの臨床. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行ら: "FcレセプターによるB細胞の抑制" 臨床免疫. 29. 550-555 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプターによる免疫制御機構" 生化学. 69. 394-409 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプターの新展開" 感染・炎症・免疫. 27. 80-89 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプターとアレルギー" 日本皮膚科学会雑誌. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行ら: "Fcγ受容体" 現代医療. 30. 290-293 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "FcRノックアウトマウスを用いたアレルギー発症機構の解析" アレルギー科. 4. 471-477 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行: "FcRとKIRとの境目" JSI New sletter. 5. 5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Takai,et al.: "Regulation of murine hypersensitive responses by Fc receptors." Allergology International. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行ら: "アレルギーとFcレセプター" Molecular Medicine. 34. 1464-1476 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hayami,K.,et al.: "Molecular cloning of a novel murine cell-surface glycoprotein homologous to killer cell inhibitory receptors." J.Biol.Chem.272. 7320-7327 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hikida,M.,et al.: "Characterization of B cells expressing recombination activating genes in germinal centers of immunized mouse lymph nodes." J.Immunol.158. 2509-2512 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kuroiwa,A.,et al.: "Association of tyrosine phosphatases SHP-1 and SHP-2,and inositol 5-phosphatase SHIP with gp49B1,and chromosomal assignment of the gene." J.Biol.Chem.273. 1070-1074 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamashita,Y.,et al.: "Association of tyrosine-phosphorylated cytoplasmic motifs of p91A with tyrosine-and inositol-phosphatases." J.Biochem.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Watanabe,N.,et al.: "Antigen receptor cross-linking by anti-immunoglobulin antibodies coupled to cell surface membrane induces rapid apoptosis of normal spleen B cells." Scand.J.Immunol.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda,A.,et al.: "Requirement of tyrosine phosphatase SHP-1 and SHP-2 for PIR-B-mediated inhibitory signal." J.Exp.Med.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "標準免疫学" 医学書院, 390-401 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "実験医学別冊「免疫研究の最前線'97-'98」" 羊土社, 172-179 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Takai and J.V.Ravetch: "Immunoglobulin Receptors and their Physiological and Pathological Roles in Immunity" Eds J.G.J.van de Winkel and P.Mark Hogarth,Kluwer Academic Publishers,((印刷中))

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "Annual Review 免疫1998" 中外医学社, 312-320 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "「ノックアウトマウス・データブック」" 中山書店, ((印刷中))

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Takai(編集協力): "Edited by Tak W.Mak,Academic Press" Knockout Mice., ((印刷中))

    • Related Report
      1997 Annual Research Report
  • [Publications] Takai,T.: "Augmented humoral and anaphylactic responses in Fcγ RII-deficient mice." Nature. 379. 346-349 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hikida,M.: "Requirements of a costimulus for IL-4-induced IgE class switching in murine B cells activated via antigen receptors : Effectiveness of 8-mercaptoguanosine." J.Immunol.156. 2730-2736 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hikida,M.: "Reexpression of RAG-1 and RAG-2 Genes in Activated Mature Murine B cells." Scince. 274. 2092-2094 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayami,K.: "Molecular cloning of a novel murine cell-surface glycoprotein homologous to killer cell inhibitory receptors." J.Biol.Chem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Hikida,M.: "Characterization of B cells expressing recombination activating genes in germinal centers of immunized mouse lymph nodes." J.Immunol.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Takai,T.: "Multiple loss of effector cell functions in FcRγ-deficient mice." Intern.Rev.Immunol.13. 369-381 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "IgGのFcレセプターとアレルギー" 臨床免疫. 28. 1062-1068 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "FcγRとアレルギー(とくにFcγRII)" アレルギー科. 2. 136-143 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプター・ノックアウトマウス" 免疫・Immunology Frontier. 6. 153-158 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "FcγRIIノックアウトマウスにおけるマスト細胞の異常活性化" 臨床免疫. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプターと抗体産生制御" 臨床免疫. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "ノックアウトマウスによる免疫研究" 実験医学. 15. 50-54 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプター欠損マウス" Molecular Medicine. 34. 44-54 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "FcRγ鎖ノックアウトマウスによるアレルギー発症機構の解析" アレルギー臨床. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "FcレセプターによるB細胞の抑制" 臨床免疫. 印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行: "Fcレセプターによる免疫制御機構" 生化学. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "免疫'96-97" 中山書店, 9 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "分子アレルギー学" メディカルレビュー社(印刷中),

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井俊行(分担執筆): "標準免疫学" 医学書院(印刷中),

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiyuki Takai (分担執筆): "Immunoglobulin Receptors and their Physiological and Pathological Roles in Immunity" Kluwer academic publishers (印刷中),

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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