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Abundant expression of transcription factor GATA-2 in proliferating but not differentiated mast cells in tissues of mice

Research Project

Project/Area Number 08670248
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

JIPPO Tomoko  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70252658)

Co-Investigator(Kenkyū-buntansha) KITAMURA Yukihiko  Osaka University Medical School, Professor, 医学部, 教授 (70028520)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsmast cell / transcription factor / cell proliferation / cell differentiation / in situ hybridization / development / GATA / mast cell-deficient mouse / GATA配列
Research Abstract

Although GATA-binding transcription factors (GATA-1 and GATA-2) are strongly expressed in cultured mast cells (CMCs), their expression in mast cells within tissues has not been reported. We examined the expression of GATA-1 and GATA-2 in skin tissues of mice using Northern blot analysis and in situ hybridization. Messenger (m) RNA for GATA-2 but not for GATA-1 was expressed in skin mast cells of WB-+/+ embryos between days 15 and 17 postcoitum (pc). The expression was downregulated on and after day 18 pc. Skin mast cells did not express GATA-2 after birth, either. When the number of skin mast cells was compared with the number of GATA-2 mRNA-expressing cells, GATA-2 mRNA appeared to be expressed by mast cells only when the number was increasing. When the mRNA expression of high affinity IgE receptor beta-subunit and mast cell carboxypeptidase A was used as differentiation markers, the expression of these mRNAs continued even after the downregulation of GATA-2 expression. To clarify the relationship of the proliferation and GATA-2 expression, proliferating CMCs derived from WBB6F_1-+/+mice were transplanted into the peritoneal cavity of mast cell-deficient WBB6F_1-W/W^vmice. The CMCs stopped both the proliferation and GATA-2 expression after the transplantation, suggesting the association of these two parameters in mast cells within tissues of mice.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Jippo T: "Involvement of transcription factor encoded by the mouse mi locus (mitf) in expression of p75 receptor of nerve growth factor in cultured mast cells of mice" Blood. 90・7. 2601-2608 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morii E: "abnormal expression of mouse mast cell protease 5 gene in cultured mast cells derived from mutant mi/mi mice" Blood. 90・8. 3057-3066 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jippo T: "Expression of mast-cell-specific proteases in tissues of mice studied by in situ hybridization" Am J Pathbol. 150・4. 1373-1382 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morii E: "Regulation of mast cell protease 6 gene expression by transcription factor encoded by the mi locus" Blood. 88・7. 2488-2494 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Lee YM: "Inhibition of histamine synthesis by glycyrrhetinic acid in mast cells cocultured with Swiss 3T3 fibroblasts" Int Arch Allergy Immunol. 110. 272-277 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jippo T: "abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice" Blood. 87・3. 993-998 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitamura Y: "Mast cells and basophils" Marcel dekker,New York, 300 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 北村 幸彦: "血液・免疫・腫瘍" 株式会社 メディカルレビュー社, 132 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jippo T: "Abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice : Demonstration by in situ hybridization." Blood. 87. 993-998 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Lee YM: "Inhibition of histamine synthesis by glycyrrhetinic acid in mast cell cocultured with Swiss 3T3 fibroblasts." Int Arch Allergy Immunol. 110. 272-277 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morii E: "Regulation of mouse mast cell protease 6 gene expression by transcription factor encoded by the mi locus." Blood. 88. 2488-2494 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jippo T: "Expression of mast-cell-specific proteases in tissues of mice studied by in situ hybridization." Am J Pathol. 150. 1373-1382 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jippo T: "Involvement of transcription factor encoded by the mouse mi locus (MITF) in expression of p75 receptor of nerve growth factor in cultured mast cells of mice." Blood. 90. 2601-2608 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morii E: "Abnormal expression of mouse mast cell protease 5 gene in cultured mast cells derived from mutant mi/mi mice." Blood. 90. 3057-3066 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jippo T: "Involvement of franscription foctor encoded by the mouse mi locus(MITF)in expression of P75 receptor of nerve growth factor in cnthecd mast cells of mice" Blood. 90・7. 2601-2608 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morii E: "Abnormal expression of mouse mast cell protease 5 gene in cultured mast cells derived from mutnnt mi/mi mice" Blood. 90・8. 3057-3066 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jippo T: "Expression of mast-cell-specific proteasrs in tissucs of mice sludied by in situ hybrldization" Am J Pathol. 150・4. 1373-1382 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morii E: "Regulation of mase cell protease 6 gene expression by transcription factor cncodod by the mi locus" Blood. 88・7. 2488-2494 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Lee YM: "Inhibition of histamine synthesis by glyeyrrhetinic acid in mase cells cocultured with Swiss 3t3 fibroblasts." Int Arch Allergy Ommunol. 110. 272-277 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jippo T: "Abundant expression of transcriphum factor GATA-2 in proliferating but not in differentiated mast cells in tissucs of mice : Demonstration by in situ hybridization" Blood. 87・3. 993-998 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitamura Y: "Mast cells and basoplils" Marcel dekker,New York, 300 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 北村幸彦: "血液・免疫・腫瘍" 株式会社 メディカルレビュー社, 132 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jippo T: "Abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice : demonstration by in situ hybridization" Blood. 87. 993-998 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zee YM: "Inhibition of histamine synthesis by glycyrrhetinic acid mast cell cocultured with Swiss 3T3 fibroblasts." Int Arch Allergy Ommunol. 110. 272-277 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Morri E: "Regulation of mouse mast cell protease 6 gene expression by transcription factor encoded by the mi locus" Blood. 88. 2488-2494 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsujino K: "Establishment and characterization of cell lines derived from a transplantable rat malignant meningioma : morphological heterogeneity and production of nerre grouth factor" Acta Neuropathol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Jippo T: "Factors that influonce expression of mast cell specfei proteases in tissues of mice studied by in situ hybridization histochemistry" (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitamura Y: "Mast cells and basoplils" marcel dekker,New York, 300 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北村 幸彦: "血液・免疫・腫瘍" 株式会社メディカルレビュー社, 132 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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