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Role of Stress Protein on Bacterial Infection

Research Project

Project/Area Number 08670317
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyorin University

Principal Investigator

YAMAMOTO Tomoko  Kyorin Univ.Sch.of Med., Associate Professor, 医学部, 助教授 (60110342)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Hiroyuki  Kyorin Univ.Sch.of Med.Assistant Professor, 医学部, 助手 (40221650)
TAGUCHI Haruhiko  Kyorin Univ.Sch.of Med.Lecturer, 医学部, 講師 (20146541)
HANAWA Tomoko  Kyorin Univ.Sch.of Med.Assistant Professor, 医学部, 助手 (80255405)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsstress protein / bacterial virulence / macrophage / Y.enterocolitica / L.monocytogenes / phagocytosis / シャペロン / 病原性 / エルシニア / リステリア / 細菌病原因子
Research Abstract

All organisms respond to unfavorable conditions such as stressful environment by the rapid and transient acceleration in the synthesis of a group of proteins called the stress proteins. For pathogenic bacteria, the intracellular environment of macrophages is one of the most hostile environments. Therefore, the bacteria in the phagocytes may respond to the hostile stimuli by the expression of the stress proteins, and those pro. teiris may put the intracellular bacteria at advantages in the survival in the phagocytes. To elucidate the role of bacterial stress protein on the survival in the macrophages and expression of virulence, we studied the stress response and the role of stress proteins of Yersinia enterocolitica and Listeria monocytogenes within macrophages after phagocytosis. The results are as follows ; (1) The Y.enterocolitica gsrLAMBDA was identified as essential for protecting cells under both extracellular environmental stress and intracellular stress in macrophages due to phagocytosis. The gsrLAMBDA encodes a basic 49.5 kDa protein which is a periplasmic protease. (2) The GsrA stress protein was induced by macrophage phagocytosis. (3) L.monocylogenes could grow in macrophages without the induction of stress proteins. (4) The dnaK gene was cloned and subjected to the molecular analysis to understand the role of stress protein DnaK for intracellular survival of L.monocylogenes . (5) The DnaK does not largely contribute to the survival of L.inonocytogenes in macrophage cells but is involved in the step of the phagocytosis. From these results, it is speculated that common mechanisms with the stress proteins would contribute to the expression of the bacterial pathogen esis besides the species-specific mechanisms.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] T.Yamamoto, T.Hanawa, S.Ogata & S.Kamiya: "Idcntitication and characterization of the Yersinia enterocolitica gsr Agene which protectivelyresponds to intracellular stress induced by macrophagc-phagocytosis and to extracellular environmental stresses." Infection and Immunity. 64. 2980-2987 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本 友子: "細菌の食菌抵抗性とストレス蛋白質" 医学のあゆみ. 178. 446-447 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山本 友子: "病原細菌のストレス応答-ストレス蛋白質は病原因子か?" 日本細菌学会誌. 51. 1025-1036 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Yamamoto, T.Hanawa & S.Kamiya.: "The Yersinia enterocoliticu GsrA protein, involved in intracellular survival, is induced by macrophage-phagocytosis." Infection and Immunity. 65. 2190-2196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Hanawa, et al.: "The Listeria monocytogenes DnaK chaperone is required for stress tolerance and efficient phagocytosis with macrophages." Cell Stress & Chaperones. 4(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Yamamoto, Tomoko Hanawa, Sachio Ogata, and Shigeru Kamiya.: "Identification and characterization of the Yersinia enterocolitica gsrA gene which protectively responds to intracellular stress induced by macrophage-phagocytosis and to extracellular environmental stresses." Infect.Immun.64. 2980-2987 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Yamamoto: "Bacterial esistance to phagocytosis and stress proteins" Igakunoayumi. 178. 446-447 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Yamamoto: "Stress response of pathogenic bacteria : Are stress proteins virulence factors ?" Nihonsaikingakuzasshi. 51. 1025-1036 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Yamamoto, Tomoko Hanawa and Shigeru Kamiya: "The Yersinia enterocolitica GsrA protein, involved in intracellular survival, is induced by macrophage-phagocytosis" Infect.Immun. 65. 2190-2196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoko Hanawa, Minoru Fukuda, Hayato Kawakami, Hiroshi Hirano, Shigeru Kamiya and Tomoko Yamamoto: "The Listeria monocytogenes DnaK chaperone is required for stress tolerance and efficient phagocytosis with macrophages" Cell Stress & Chaperones. 4 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Hanawa,et al.: "The Listeria monocytogenes DnaK chaperone is required for stress tolerance and efficient phagocytosis with macrophages." Cell Stress & Chaperones. 4(in press.). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamamoto,T.et al.: "The Yersinia enterocolitica GsrA stress protein,involved in intracellular survival,is induced by macrophage-phagocytosis." Infect.Immun.65. 2190-2196 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,H.et al.: "Growth inhibition of Helicobacter pylori by monoclonal antibody to heat-shock protein 60." Microbiol.Immunol.41. 909-916 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,H.et al.: "Production and characterization of monoclonal antibodies to heat-shock protein 60 of Helicobacter pylori." J.Med.Microbiol.46. 819-824 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,H.et al.: "Heat-shock protein 60 homologue of Helicobacter pylori is associated with adhesion of H.Pylori to human gastric epithelial cells." J.Med.Microbiol.46. 825-831 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto T et al.: "Identification and characterization of the Yersinia enterocolitica gsrA gene,which protectively responds to intracellular stress induced by macrophage phagocylosis and extracellular enviromental stress." Infect.Immun.64. 2980-2987 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山本友子: "(総説)病原細菌のストレス応答-ストレス蛋白質は病原因子か?" 日本細菌学雑誌. 51. 1025-1036 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi H et al.: "Induction and epitope analysis of heat shock protein of Helicobacter pylori." J.Gastroenterol.31. 12-15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi H et al.: "Analysis of the epitopes recognized by mouse monoclonal antibodies directed to Yersinia enterocolitica heat-shock protein 60." Microbiol.Immunol.40. 77-80 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi H et al.: "Flow cytometric analysis of the heat shock protein 60 expressed on the cell surface of Helicobacter pylori." J.Med.Microbiol.45. 270-277 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taguchi H et al.: "Immunocytochemical localization of 60-kDa heat shock protein in Vibrio cholerae." Z.Bakteriol.284. 496-500 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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