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MOLECULAR BASIS OF NOVEL RECEPTOR BINDING SPECIFICITY OF RECENT ISOLATES OF HUMAN INFLUENZA A VIRUSES

Research Project

Project/Area Number 08670349
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

NOBUSAWA Eri  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,DEPARTMENT OF VIROLOGY,ASSISTANT PROFESSOR, 医学部, 講師 (90183904)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Katsuhisa  NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,DEPARTMENT OF VIROLOGY,PROFESSOR, 医学部, 教授 (40012778)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsinfluenza virus / receptor / hemagglutinin / インフルエンザウィルス / 血球凝集能 / レセプター結合特異性
Research Abstract

Human influenza A viruses including H1 and H3 subtypes isolated after 1991 lost their ability to agglutinate chicken red blood cells (CRBC). The hemagglutinin (HA) molecules of those viruses were expressed on the Cos cells and their ability to bind to CRBC was examined. Study of chimeric and site specific mutant HAs showed that the amino acid changes at position 225 appearing after 1986 was responsible for loss of the ability of H1 HA to CRBC.Phylogenetic study showed this change has been retained among all H1 HAs up to 1995. On the other hand amino acid change at position 190 appearing after 1990 was responsible for the loss of the ability of H3 HAs to CRBC and this change has been retained among all H3 HA up to 1997.
On the other hand H3 viruses which do not agglutinate not only CRBC but goose red blood cells (GRBC) were isolated after 1996. To investigate the receptor binding ability of HA proteins of these viruses, we compare the ability to bind to GRBC between the HA proteins isolated before and after 1996. Results showed that amino acid changes at positions 189 and 190 on HAs of the viruses isolated before 1996 were responsible for the loss of the ability to bind to GRBC,while the amino acid change at position 226 of HAs of the viruses isolated after 1996 was responsible for the loss of the ability to bind to GRBC.Sialidase treatment of the Cos cells expressing HA of the viruses isolated before 1996 was effective for the HA to gain the ability to bind to GRBC,suggesting that the amino acid changes at position 189 and 190 affected the glycosylation of the HA.This study shows that recent influenza viruses have been changing the receptor binding specificity by substituting the amino acids locating on the receptor binding site of HA.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(12 results)

All Other

All Publications (12 results)

  • [Publications] Takayuki Morishita: "Studies on the molecular basis for loss of the ability of recent influenza A(HINI)virus strains to agglutinate chicken." Journal of General Virology. 77. 2499-2506 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takayuki Morishita: "Analysis of the host-specific haemagglutination of influenza A(HINI)viruses isolated in the 1995/6 season." Epidemiol.Infect.119. 327-334 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitsuo Takahashi: "Influenza A virus Infection of Primary Cultured cells From Rat Fetal Brain" Parkinsonism & Related Disorders. Vol.3 No.2. 97-102 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Eri Nobusawa: "Options for the Control of Influenza III" Elsevier(EXCERDTA MEDICA), 6 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takayuki Morishita, Eri Nobusawa, Katsuhisa Nakajima and Setsuko Nakajima: "Studies on the molecular basis for loss of the ability of recent influenza A (H1N1) virus strains to agglutinate chicken erythrocytes." Journal of General virology. vol.77. 2499-2506 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Eri nobusawa, Takayuki Morishita and Katsuhisa Nakajima: "Novel receptor binding specificity of recent isolates of human influenza A viruses." Elsevier Science Options for the control of influenza. III. 437-442 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Morishita, E.Nobusawa, S.Luo, K.Sato, S.Nakajima and K.Nakajima: "Analysis of the host-specific haemagglutination of influenza A (H1N1) viruses isolated in the 1995/6 season." Epidemiol.Infect.vol.119. 327-334 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitsuo Takahashi, Tatsuo Yamada, Keiko Nakanishi, Kaori Fujita, Katsuhisa Nakajima, Eri Nobusawa, Takayuki Yamamoto, Taiji Kato, and Hidechika Okada: "Influenza A virus infection of primary cultured cells from rat fetal brain" Parkinsonism and Related Disorders. vol.3. 97-102 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Morishita: "Analysis of the host-specific haemagglutination of influenza A(HINI) viruses isolated in the 1995/6 season" Epidemiol.Infect.119. 327-334 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mitsuo Takahashi: "Influenza A Virus Infection of Primary Cultured Cells From Rat Fetal Brain" Parkinsonism & Related Disorders. Vol.3 No.2. 97-102 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tukayuki Morishita: "Studies on the molecular basis for loss of the ability of recent influenza A (HINI) virus strains to agglufinafe chicken" Journal of General Virology. 77. 2499-2506 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Eri Nobusawa: "Options for the Control of Influenza III" Elsevier, 6 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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