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Racial discrimination by direct sequencing of human mitochondrial DNA

Research Project

Project/Area Number 08670484
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionOsaka University

Principal Investigator

HONDA Katsuya  Osaka University Medical School, Associate Professor, 医学部, 助教授 (00240789)

Co-Investigator(Kenkyū-buntansha) NAKATOME Masato  Osaka University Medical School, Assistant Professor, 医学部, 助手 (90263251)
HARIHARA Shinji  The University of Tokyo, Department of Biological Science, Assistant Professor, 大学院・理学系研究科, 助手 (40198932)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmitochondrial DNA / Control region / Asian population / Sequence rariation / DNA typing / chelex extraction / ミトコンドリア / DNA / 遺伝的多型 / 人種 / シークエンス変異 / アジア人 / 人類遺伝学
Research Abstract

Hypervariable region of the human mitochondrial DNA was sequenced from 200 samples in several asian populations (Japan, China, Mongolia, Myanmar, Taiwan, Malaysia, and Bangladesh). DNA were extracted from 200 salivas in solution of 5% chelex. PCR were performed using primers L15996 and H408. The sequence variants of the hypervariable regions were detected by fluorescence with a DNA-sequencer (ABI PRISM ; model 377) after cycle sequencing reaction using dye-primer method. We identified distribution and frequency of sequence variations and selected 12 nucleotide in 35bp of the highest variable regions (16158-16192). In these sequence, we devided major three types and three variants types. Frequency of each types were different among asian populations. In Japanese, Anderson's basic type was the most frequent of 65.7%. Unique variation were encountered in in Malaysian and Myanmar populations.
We are investigating of true polymorphic site and frequency of polymorphism with realible method. As a results, our findings show almost similar polymorphic pattern was found within Asian population. Then we can grouped major 5 types of mtDNA.Also, we can find that mt-DNA sequencing is not always highly discriminative among the same populations, but is more informative between people from different racial origins.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Honda, K et al.: "Detection of Sequence variants in hypervariable segments of mitochondrial DNA in the Asian population" Advances in Forensic Huemogenetics. 6. 29-32 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda, K et al.: "A variation map based on nucleotide sequences of mt-DNA control region of Asian population" Advances in Research on DNA polymorphism. 1. 180-183 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda, K et al.: "A rapid and simple procedure of sex identification from the DNA in a trace amount of saliva vsing PCR" International Medical Journal. 4. 227-228 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 本田 克也 ほか: "ロング・シークエンシングによるミトコンドリアDNA高変異領域の多型検出" DNA多型. 5. 181-183 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda, K,et al.: "Detection of sequence variants in hypervariable seqments of mitochondrial DNA in the Asian population" Advances in Forensic Haemogenetics. 6. 29-32 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda K,et al.: "Avariation map based on nucleotide sequences of mt-DNA Control regionof Asian population" Advances in Research on DNA polymorphism. 1. 336-339 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda K,et al.: "A rapid and simple procedure of sex identification from the DNA in a trace amount of saliva using the polymerase chain reaction" International Medical Journal. 4. 227-228 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Honda,K etal.: "Detection of sequence variants in hypervariable segments of mitochondrial DNA in the Asian population" Advances in Forensic Haemogenetics. 6. 29-32 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Honda,K etal.: "A variation map based on nucleotide sequences of mt-DNA control region of Asian population" Advances in Research on DNA polymorphism. 1. 180-183 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Honda,K etal.: "A rapid and simple procedure of sex identification from the DNA in a trace amourt of saliva vsing PCR" International Medical Journal. 4. 227-228 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 本田克也 ほか: "ロング・シークエンシングによるミトコンドリアDNA高変異領域の多型検出" DNA多型. 5. 181-183 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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