|Budget Amount *help
¥2,700,000 (Direct Cost : ¥2,700,000)
Fiscal Year 1997 : ¥1,800,000 (Direct Cost : ¥1,800,000)
Fiscal Year 1996 : ¥900,000 (Direct Cost : ¥900,000)
We examined apoptosis and immune deviation in intrathyroidal lymphocytes from patients with autoimmune thyroid disease. Apoptosis of intrathyroidal lymphocytes was confirmed by examining the DNA fragmentation by TUNEL method, the chromatin condensation and fragmentation by chromatin staining with bisbenzimide H33258, and the abnormal integrity of cell membrane by 7-amino-actinomycin D.The expression of Fas molecules on intrathyroidal lymphocytes was also examined by immunohistochemical staining, and found to be increased in autoimmune thyroid disease. Furthermore, we examined imbalance between the numbers of Th1 and Th2 cells and between those of Tc1 and Tc2 cells by analyzing CD4 or CD8 cells with cytoplasmic cytokines such as IFNg, IL-2, IL-4, IL-5, or IL-10, with a flow cytometer. However, we found no significant results in autoimmune thyroid disease, because of large variation among patients and of small number of patients examined. The effect of MHC class II expression on thyrocytes on apoptosis and immune deviation of lymphocytes was not efficiently examined because of small recovery of lymphocytes after co-culture of lymphocytes and thyrocytes expressing MHC class II molecules.