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Study on mechanisms of biliary phospholipid species subselection

Research Project

Project/Area Number 08670597
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TAZUMA Susumu  HIROSHIMA UNIVERSITY University Medicine Hospital Research Associate, 医学部・附属病院, 助手 (80201614)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Gunji  HIROSHIMA UNIVERSITY University Medicine Hospital Resident, 医学部・附属病院, 医員
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsbile acid / canaliculus / intrahepatic transport / phospholipid / bile secretion / vesicle / liposome / fatty acylchain / 胆汁脂質 / 肝ミクロソーム / 肝毛細胆管膜 / フォスファチジルセリン / フォスファチジルコリン / 有機陰イオン
Research Abstract

This study was undertaken to clarify factors affecting biliary phospholipid (PL) species subselection using canalicular membrane (CMV) vesicles prepared from control or cholestatic Sprague-Dawley rats. The fusibility of liposomes with CMV was inversely correlated with hydrophobic index of PC molecules determined by HPLC.In contrast, the hydrophobicity of PC molecules of canalicular bilayrs increased under cholestasis, and the fusibility between CMV and liposomes was reduced. This phenomenon associated with a reduction of hydrophobicity of sphingomyelin (SM) as reflected by a relatively low saturated/unsaturated fatty acid ratio. These data suggest that hydrophilic PL species should be preferentially secreted into bile under cholestasis, and that this may result in retaining of hydrophobic PL species within a canalicular membrane, and thereby intrahepatic lipids reflex to sinusoidal site because of reduced fusibility of vesicles with a canalicular membrane inner layr. Thus, the regulation of PC species distribution within hepatic membranes plays an important role in intrahepatic lipid transport.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(41 results)

All Other

All Publications

  • [Publications] Hidenori Ochi: "Lecithin hydrophobicity modulates the process of cholesterol crystal nucleation and growth in supersaturated model bile systems" Biochemical J. 318. 139-144 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshifumi Yamashita: "Method for quantitative assessment of vesicle transformation in supersaturated model bile systems" J Gastroenterology Hepatology. 11. 864-869 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Miura: "Effect of organic anions on acyl chain composition of secreted biliary lecithin in rats : Relation to hepatocellular vesicle fusion" International Hepatology Communications. 6. 191-198 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naomichi Hirano: "Transcytotic vesicle fusion with canalicular membranes is modulated by phospholipid species : Implications for biliary lipid secretion" J Gastroenterology Hepatology. 12. 534-539 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Susumu Tazuma: "A quantitative assessment of serum chylomicron by light scattering intensity : application to the intestinal fat absorption test" J Gastroenterology Hepatology. 12. 713-718 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Miura: "Effect of cholestasis induced by organic anion on the lipid composition of hepatic membrane subfractions and bile in rats" J Gastroenterology Hepatology. 12. 734-739 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Miura: "Extracellular and intracellular regulation of biliary lecithin hydrophobicity : relation to organic anion- or papaverine-induced uncoupling phenomenon" Digestive Diseases and Science. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroaki Miyake: "Bile salt hydrophobicity modulates subselection of biliary lecithin species in bile salt pool-deplated rats" Digestive Deseases and Science. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Susumu Tazuma: "Tauroursodeoxycholate and taurochenodeoxycholate stabilize bile lipid metastability through different mechanisms : relation to phospholipid fatty acid composition" Hepatology Research. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Susumu Tazuma: "A combination therapy of simvastain and ursodeoxycholic acid is more effective on cholesterol gallstone dissolution rates than ursodeoxycholic acid monotherapy" J Clinical Gastroenterology. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoji Nishioka: "Quantitative assessment on comparative, potencies of cholesterol crystal promoting factors : relation to mechanistic characterization" Biochemical J.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Susumu Tazuma: "Clinical Studies in Medical Biochemistry" Oxford university press inc. (New York). Glew, RH & Ninomiya, Y (2nd eds), 380 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ochi H,Tazuma S,et al: "Lecithin hydrophobicity modulates the process of cholesterol crystal nucleation and growth in supersaturated model bile systems." Biochemical J. 318. 139-144 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamashita Y,tazuma S,et al: "Method for quantitative assessment of vesicle transformation in supersaturated model bile systems." J.Gastroenterology Hepatology. 11. 864-869 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura H,Tazuma S,et al: "Effects of organic anions on acyl chain composition of secreted biliary lecithin in rats : Relation to hepato-cellular vesicle pathway." Internaitonal Hepatology Communications. 6. 191-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirano N,Tazuma S,et al: "Transcytotic vesicle fusion with canalicular membranes is modulated by phospholipid species : Implicatoins for biliary lipid secretion." J Gastroenterol Hepatology. 12. 534-539 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tazuma S,et al: "A quantitative assessment of serum chylomicron by light scattering intensity : application to the intestinal fat absorption test." J Gastroenterol Hapatology. 12. 713-718 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura H,Tazuma S,et al: "Effect of cholestasis induced by organic anion on the lipid composition of hepatic membrane subfractions and bile in rats." J Gastroenterol Hepatology. 12. 734-739 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura H,Tazuma S,et al: "Extracellular and intracellular regulation of bilary lecithin hydrophobicity : relation to organic anion- or papaverine-induced uncoupling phenomenon." Digestive Diseases and Science. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyake H,Tazuma S,et al: "Bile salt hydrophobicity modulates subselection of biliary lecithin species in bile salt pool-depleted rats." Digestive Diseases and Science. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tazuma S,et al: "Tauroursodeoxycholate and taurochenodeoxycholate stabilize bile lipid metastability through different mechanisms : relation to phospholipid fatty acid composition." Hepatology Research. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tazuma S,et al: "A combination therapy of simvastatin and ursodeoxycholic acid is more effective on cholesterol gallstone dissolution rates than ursodeoxycholic acid monotherapy." J Clinical Gastroenterology. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishioka T,Tazuma S,et al: "Quantitative assessment on comparative potencies of cholesterol crystal promoting factors : relation to mechanistic characterization" Biochemical J. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tazuma S,et al: Jaundice and gallstone disease. Clinical Studies in Medical Biochemistry.In Glew, RH & Ninomiya, Y (2nd eds) New York, Oxford University Press Inc., 110-121 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Miura: "Effect of organic anions on acyl chain composition of secreted biliary lecithin in rats : Relation to hepatocellular vesicle pathway." International Hepatology communications. 6. 191-198 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naomichi Hirano: "Transcytotic vesicle fusion with canalicular membranes is modulated by phospholipid species : Implications for biliary lipid secretion." J Gastroenterology Hepatology. 12. 534-539 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Tazuma: "A quantitative assessment of serum chylomicron by light scattering intensity : application to the intestinal fat absorption test." J Gastroenterology Hepatology. 12. 713-718 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroyuki Miura: "Effect of cholestasis induced by organic anion on the lipid composition of hepatic membrane subfractions and bile in rats." J Gastroenterology Hepatology. 12. 734-739 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroyuki Miura: "Extracellular and intracellular regulation of biliary lecithin hydrophobicity : relation to organic anion^- or papaverine^- induced uncoupling phenomenon." Digestive Diseases and Science. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroaki Miyake: "Bile salt hydrophobicity modulates subselection of biliary lecithinspecies in bile salt pool-deplated rats." Digestive Diseases and Science. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Tazuma: "Tauroursodeoxycholate and taurochenodeoxycholate stabilize bile lipid metastability through different mechanism : relation to phospholipid fatty acid compositon." Hepatology Research. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Tazuma: "A combination therapy of simvastain and ursodeoxycholicacid is more effective on cholesterol gallstone dissolution rates than ursodeoxycholic acid menotherapy." J Clinical Gastroenterology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomoji Nishioka: "Quantitative assessment on comparative potencies of cholesterol crystal promoting factors : relation to mechanistic characterization." Biochemchal J. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Tazuma: "Clinical Studies in Medical Biochemistry" Oxford university Press Inc.(New York). Glew,RH & Ninomiya, Y (2nd eds)., 380 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] HIDENORI OCHI: "LECITHIN HYDROPHOBICITY MODULATES THE PROCESS OF CHOLESTEROL CRYSTAL NUCLEATION AND GROWTH IN SUPERSATURATED MODEL BILE SYSTEMS" Biochem.J.318. 139-144 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] YOSHIHUMI YAMASHITA: "METHOD FOR QUANTITATIVE ASSESSMENT OF TRANSFORMATION OF NON-MICELLAR CHOLESTEROL CARRIERS IN MODEL BILE SYSTEMS" J.Gastroenterol.Hepatol.11. 864-869 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] GUNJI YAMASHITA: "COMPARISON OF HAPTOGLOBIN AND APOLIPOPROTEIN A-1 ON BILIARY LIPID PARTICLES INVOLVED IN CHOLESTEROL CRYSTALLIZATION" J.Gastroenterol.Hepatol.11. 738-745 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] SUSUMU TAZUMA: "EFFICACY OF ENDOSCOPIC ULTRASONOGRAPHY IN THE DIAGNOSIS OF LATERAL LYMPHATIC SPREAD IN RECTAL CANCER" Dig.Endosc.8-1. 18-21 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] HIROYUKI MIURA: "EFFECT OF ORGANIC ANIONS ON ACYL CHAIN COMPOSITION OF SECRETED BILIARY LECITHIN IN RATS : RELATION TO HEPATOCELLULAR VESICLE PATHWAY" International Hepatology Comunications. 6. 191-198 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] NAOMICHI HIRANO: "TRANSCYTOTIC VESICLE FUSION WITH CANALICULAR MEMBRANES IS MODULATED BY PHOSPHOLIPID SPECIES : IMPLICATIONS FOR BILIARY LIPID SECRETION" J.Gastroenterol.Hepatol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] SUSUMU TAZUMA: "JAUNDICE AND GALLSTONE DISEASE" New York, Oxford University Press Inc.(in press), (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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