| Project/Area Number | 08670597 |
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TAZUMA Susumu HIROSHIMA UNIVERSITY University Medicine Hospital Research Associate, 医学部・附属病院, 助手 (80201614)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Gunji HIROSHIMA UNIVERSITY University Medicine Hospital Resident, 医学部・附属病院, 医員
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed(Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1997 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1996 : ¥1,800,000 (Direct Cost : ¥1,800,000)
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| Keywords | bile acid / canaliculus / intrahepatic transport / phospholipid / bile secretion / vesicle / liposome / fatty acylchain / 胆汁脂質 / 肝ミクロソーム / 肝毛細胆管膜 / フォスファチジルセリン / フォスファチジルコリン / 有機陰イオン |
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| Research Abstract |
This study was undertaken to clarify factors affecting biliary phospholipid (PL) species subselection using canalicular membrane (CMV) vesicles prepared from control or cholestatic Sprague-Dawley rats. The fusibility of liposomes with CMV was inversely correlated with hydrophobic index of PC molecules determined by HPLC.In contrast, the hydrophobicity of PC molecules of canalicular bilayrs increased under cholestasis, and the fusibility between CMV and liposomes was reduced. This phenomenon associated with a reduction of hydrophobicity of sphingomyelin (SM) as reflected by a relatively low saturated/unsaturated fatty acid ratio. These data suggest that hydrophilic PL species should be preferentially secreted into bile under cholestasis, and that this may result in retaining of hydrophobic PL species within a canalicular membrane, and thereby intrahepatic lipids reflex to sinusoidal site because of reduced fusibility of vesicles with a canalicular membrane inner layr. Thus, the regulation of PC species distribution within hepatic membranes plays an important role in intrahepatic lipid transport.
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