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Release of granulocyte-mecrophage colony-stimulating factor stimulatd with eosinphil granule proteins and its signal transduction

Research Project

Project/Area Number 08670676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionDokkyo Univ.Sch.of Med.

Principal Investigator

MOTOJIMA Shinji  Dokkyo Univ Sch Med Intern Med, Ass.Prof., 医学部, 助教授 (90157842)

Co-Investigator(Kenkyū-buntansha) HIRATA Akira  Dokkyo Univ Sch Med Intern Med, Instructor, 医学部, 助手 (00218778)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsairway epithelium / eosinophil / GM-CSF / eosinphil peroxidase / major basic protein / tyrosine phosphorylation / Eosinophil Perondase / Granulocyte-macrophage colony-stimulatir,factor / 気道上皮細胞 / 顆粒蛋白
Research Abstract

In the study of the previous year, we have found that eosinophil-derived basic proteins, namely eosinophil peroxidase (EPO) and major basic protein (MBP), stimulate the release of granulocyte-macrophage colony-stimulating factor (GM-CSF) from bronchial epithelial cells. This year we studied whether tyrosine phosphorylation is involved in the release of GM-CSF.The bronchial epithelial cells were stimulated with EPO (5.9x 10^<-7>M) in the presence or absence of tyrosine phosphorylation inhibitor, herbimycin A (0.2-2.0mug/ml) or genistein (10-100mug/ml), and the release of GM-CSF was evaluated. Both of the tyrosine phosphorylation inhibitors inhibited the release of GM-CSF from the epithelial cells in a concentration-dependent manner. Moreover, the GM-CSF release with IL-1beta and TNF-alpha, very potent stimuli for GM-CSF release, was also completely inhibited with these inhibitors. The proteins which are tyrosine-phosphorylated in terms of molecular weight revealed the same in the stimulation of IL-1beta and EPO by the use of Western blot. Although the GM-CSF release by EPO or MBP does not seem to involve specific receptors, the same signal transduction pathway seems to be utilized as in the case of IL-1beta which has a specific receptor. Further studies are needed to specify the proteins which are tyrosine-phosphorylated and to clarify the signal transduction of EPO and MBP in bronchial epithelial cells in the absence of a specific receptor.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Shinji Motojima, et al.: "Eosinophil peroxidase stimulates the release of GM-CSF from bronchial epithelial cell." J Allergy Clin Immunol. 98(12-s). s216-s223 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinji Motojima, et al.: "Asthma,Inflammatory Mechanisms" Marcel Dekker,Inc (in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinji Motojima, et al.: "Eosinphil peroxidase stimulates the release of granulocyte-macrophage colony-stimulating factor from bronchial epithelial cells." J Allergy Clin Immunol. 98(12-s). s216-s223 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinji Motojima, et al.: Eosinophil Activation in asthma, in Holgate S,eds Asthma, Inflammatory Mechanisms.Marcel Dekker, Inc (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinji Motojima, et al: "Eosinophil peroxidase stimulates release of granulogte-macrophage colony-stimulating factor from bronchial epithelial cells" J Allergy clin Immunal. 98(6-2). S216-S223 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shinji Motojima, et al: "Asthma Inflammatory Mechanisms" Marcel Dekker,Inc(in press),

    • Related Report
      1997 Annual Research Report
  • [Publications] MOTOJIM S,et al: "Eosinophil peroxidase stimulates the release of granulogte-macrophage colony-stimulating factor from bronchid epihelial cells" J Allergy&Clinical Immunolosy. 98(6-2). S216-S223 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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